DLGAP1 Antibody | Affinity Biosciences

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Product:	DLGAP1 Antibody
Catalog:	AF0308
Description:	Rabbit polyclonal antibody to DLGAP1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	108kDa; 109kD(Calculated).
Uniprot:	O14490
RRID:	AB_2833472

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

DLGAP1 Antibody detects endogenous levels of total DLGAP1.

RRID:

AB_2833472
Cite Format: Affinity Biosciences Cat# AF0308, RRID:AB_2833472.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

4933422O14Rik; 9630002F18; AI845682; AI848168; BB075781; D17Bwg0511e; DAP 1 ALPHA; DAP 1; DAP 1 BETA; DAP-1; DAP-1-ALPHA; DAP-1-BETA; DAP1; Discs large (Drosophila) homolog associated protein 1; Discs large homolog associated protein 1; Disks large associated protein 1; Disks large associated protein 1, alpha isoform, included; Disks large associated protein 1, beta isoform, included; Disks large-associated protein 1; DLGAP 1; Dlgap1; DLGP1_HUMAN; GKAP; GKAP/SAPAP; GKPA/SAPAP; Guanylate kinase associated protein; Guanylate kinase-associated protein; hGKAP; KIAA4162; MGC88156; mKIAA4162; PSD 95/SAP90 binding protein 1; PSD-95/SAP90-binding protein 1; SAP90/PSD 95 associated protein 1; SAP90/PSD-95-associated protein 1; SAPAP 1; SAPAP1;

Immunogens

Immunogen:

Uniprot:

O14490(DLGP1_HUMAN) >>Visit HPA database.

Gene(ID):

DLGAP1(9229)

Expression:

O14490 DLGP1_HUMAN:

Expressed in brain.

Description:

SAPAP1 Part of the postsynaptic scaffold in neuronal cells. Belongs to the SAPAP family. Interacts with guanylate kinase-like domain of DLG1, DLG2, DLG3, DLG4 and AIP1. Interacts with the PDZ domain of SHANK1, SHANK2 and SHANK3. Found in a complex with DLG4 and SHANK1, SHANK2 or SHANK3. Found in a complex with DLG4 and BEGAIN.

Sequence:

O14490 DLGP1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKGLSGSRSHHHGVTCDSACDSLSHHSDRKPYLLSPVEHHPADHPYYTQRNSFQAECVGPFSDPLASSTFPRRHYTSQQELKDECALVPRTLATKANRIPANLLDQFERQLPLSRDGYHTLQYKRTAVEHRSDSPGRIRHLVHSVQKLFTKSHSLEGPSKGSVNGGKASPDEAQAARYGKRSKSKERRAEPKARPSTSPGWWSSDDNLDGDMCIYHAPSGVMTMGRCPDRSASQYFLEAYNTISEQAVKASRSNNDVKCSTCANLPVSLDTPLLKKSAWSSTLTVSRAREVYQKASVNMDQAMVKSESCQQERSCQYLQVPQDEWTGYTPRGKDDEIPCRRMRSGSYIKAMGDEDSGDSDTSPKPSPKVAARRESYLKATQPSLTELTTLKISNEHSPKLQIRSHSYLRAVSEVSINRSLDSLDPAGLLTSPKFRSRNESYMRAMSTISQVSEMEVNGQFESVCESVFSELESQAVEALDLPMPGCFRMRSHSYVRAIEKGCSQDDECVSLRSSSPPRTTTTVRTIQSSTVSSCITTYKKTPPPVPPRTTTKPFISITAQSSTESAQDAYMDGQGQRGDIISQSGLSNSTESLDSMKALTAAIEAANAQIHGPASQHMGNNTATVTTTTTIATVTTEDRKKDHFKKNRCLSIGIQVDDAEEPDKTGENKAPSKFQSVGVQVEEEKCFRRFTRSNSVTTAVQADLDFHDNLENSLESIEDNSCPGPMARQFSRDASTSTVSIQGSGNHYHACAADDDFDTDFDPSILPPPDPWIDSITEDPLEAVQRSVCHRDGHWFLKLLQAERDRMEGWCQQMEREERENNLPEDILGKIRTAVGSAQLLMAQKFYQFRELCEENLNPNAHPRPTSQDLAGFWDMLQLSIENISMKFDELHQLKANNWKQMDPLDKKERRAPPPVPKKPAKGPAPLIRERSLESSQRQEARKRLMAAKRAASVRQNSATESAESIEIYIPEAQTRL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Zebrafish

Xenopus

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O14490 As Substrate

O14490

Site	PTM Type	Source
S7	Phosphorylation	Uniprot
S24	Phosphorylation	Uniprot
S52	Phosphorylation	Uniprot
Y118	Phosphorylation	Uniprot
S154	Phosphorylation	Uniprot
S169	Phosphorylation	Uniprot
S314	Phosphorylation	Uniprot
Y317	Phosphorylation	Uniprot
S346	Phosphorylation	Uniprot
S356	Phosphorylation	Uniprot
S359	Phosphorylation	Uniprot
S375	Phosphorylation	Uniprot
S383	Phosphorylation	Uniprot
S397	Phosphorylation	Uniprot
S406	Phosphorylation	Uniprot
S412	Phosphorylation	Uniprot
S415	Phosphorylation	Uniprot
S419	Phosphorylation	Uniprot
S422	Phosphorylation	Uniprot
S440	Phosphorylation	Uniprot
S491	Phosphorylation	Uniprot
S493	Phosphorylation	Uniprot
K500	Ubiquitination	Uniprot
S503	Phosphorylation	Uniprot
S510	Phosphorylation	Uniprot
T530	Phosphorylation	Uniprot
T536	Phosphorylation	Uniprot
T537	Phosphorylation	Uniprot
T541	Phosphorylation	Uniprot
S589	Phosphorylation	Uniprot
S592	Phosphorylation	Uniprot
S595	Phosphorylation	Uniprot
S651	Phosphorylation	Uniprot
K673	Acetylation	Uniprot
S958	Phosphorylation	Uniprot

Research Backgrounds

O14490_DLGP1_HUMAN

Function:

Part of the postsynaptic scaffold in neuronal cells.

Subcellular Location:

Cell membrane>Peripheral membrane protein. Cell junction>Synapse>Postsynaptic density. Cell junction>Synapse.

Tissue Specificity:

Expressed in brain.

Subunit Structure:

Interacts with guanylate kinase-like domain of DLG1, DLG2, DLG3, DLG4 and AIP1. Interacts with the PDZ domain of SHANK1, SHANK2 and SHANK3. Found in a complex with DLG4 and SHANK1, SHANK2 or SHANK3. Found in a complex with DLG4 and BEGAIN. Interacts with DYL2 and LRFN1 (By similarity). Interacts with MPP2 (via the SH3-Guanylate kinase-like sub-module) (By similarity).

Family&Domains:

Belongs to the SAPAP family.

Research Fields

· Organismal Systems > Nervous system > Glutamatergic synapse.

References

1). Cellular and molecular landscape of mammalian sinoatrial node revealed by single-cell RNA sequencing. Nature Communications, 2021 (PubMed: 33436583) [IF=16.6]

Application: IF/ICC Species: mouse Sample: heart

Fig. 2| Expression pattern of the core cluster markers Vsnl1, Dlgap1, and Unc80 in mouse heart.f Immunofluorescence images of HCN4(green), DLGAP1 (red), and DAPI (blue).

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For Research Use Only.
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DLGAP1 Antibody - #AF0308