Product: HNF1 beta Antibody
Catalog: AF4101
Description: Rabbit polyclonal antibody to HNF1 beta
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 45,58,61kd; 61kD(Calculated).
Uniprot: P35680
RRID: AB_2835368

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
HNF1 beta Antibody detects endogenous levels of total HNF1 beta.
RRID:
AB_2835368
Cite Format: Affinity Biosciences Cat# AF4101, RRID:AB_2835368.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FJHN; Hepatocyte nuclear factor 1 beta; Hepatocyte nuclear factor 1-beta; HNF 1B; HNF 2; HNF-1-beta; HNF-1B; HNF1 beta; HNF1 homeobox B; HNF1B; HNF1B_HUMAN; HNF1beta; HNF2; Homeoprotein LF B3; Homeoprotein LFB3; HPC11; LF B3; LFB3; MODY 5; MODY5; TCF 2; TCF 2 protein; TCF-2; TCF2; TCF2 protein; Transcription factor 2; Transcription factor 2 hepatic; Variant hepatic nuclear factor 1; Variant hepatic nuclear factor; VHNF 1; vHNF1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
Transcription factor, probably binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
Sequence:
MVSKLTSLQQELLSALLSSGVTKEVLVQALEELLPSPNFGVKLETLPLSPGSGAEPDTKPVFHTLTNGHAKGRLSGDEGSEDGDDYDTPPILKELQALNTEEAAEQRAEVDRMLSEDPWRAAKMIKGYMQQHNIPQREVVDVTGLNQSHLSQHLNKGTPMKTQKRAALYTWYVRKQREILRQFNQTVQSSGNMTDKSSQDQLLFLFPEFSQQSHGPGQSDDACSEPTNKKMRRNRFKWGPASQQILYQAYDRQKNPSKEEREALVEECNRAECLQRGVSPSKAHGLGSNLVTEVRVYNWFANRRKEEAFRQKLAMDAYSSNQTHSLNPLLSHGSPHHQPSSSPPNKLSGVRYSQQGNNEITSSSTISHHGNSAMVTSQSVLQQVSPASLDPGHNLLSPDGKMISVSGGGLPPVSTLTNIHSLSHHNPQQSQNLIMTPLSGVMAIAQSLNTSQAQSVPVINSVAGSLAALQPVQFSQQLHSPHQQPLMQQSPGSHMAQQPFMAAVTQLQNSHMYAHKQEPPQYSHTSRFPSAMVVTDTSSISTLTNMSSSKQCPLQAW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35680 As Substrate

Site PTM Type Enzyme
S49 Phosphorylation
S52 Phosphorylation
S75 Phosphorylation
S80 Phosphorylation
T88 Phosphorylation
S279 Phosphorylation
S281 Phosphorylation
S288 Phosphorylation
S363 Phosphorylation
S538 Phosphorylation

Research Backgrounds

Function:

Transcription factor, probably binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Binds DNA as a dimer. Can form homodimer or heterodimer with HNF1-alpha.

Family&Domains:

Belongs to the HNF1 homeobox family.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.

Restrictive clause

 

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For Research Use Only.
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