Phospho-ATM (Ser1981) Antibody - #AF4120

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Product: Phospho-ATM (Ser1981) Antibody
Catalog: AF4120
Description: Rabbit polyclonal antibody to Phospho-ATM (Ser1981)
Application: WB IHC
Reactivity: Human
Mol.Wt.: 350kd; 351kD(Calculated).
Uniprot: Q13315
RRID: AB_2835362

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 100ul $350 In stock
 200ul $450 In stock

Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
Phospho-ATM (Ser1981) Antibody detects endogenous levels of ATM only when phosphorylated at serine 1981.
RRID:
AB_2835362
Cite Format: Affinity Biosciences Cat# AF4120, RRID:AB_2835362.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A-T mutated; A-T mutated homolog; AT mutated; AT1; ATA; Ataxia telangiectasia mutated; Ataxia telangiectasia mutated gene; Ataxia telangiectasia mutated homolog (human); Ataxia telangiectasia mutated homolog; ATC; ATD; ATDC; ATE; ATM; ATM serine/threonine kinase; ATM_HUMAN; DKFZp781A0353; MGC74674; OTTHUMP00000232981; Serine protein kinase ATM; Serine-protein kinase ATM; Serine/threonine-protein kinase ATM; Tefu; TEL1; TEL1, telomere maintenance 1, homolog; TELO1; Telomere fusion protein;

Immunogens

Immunogen:
Uniprot:

Q13315(ATM_HUMAN) >>Visit HPA database.

Gene(ID):
ATM(472)
Expression:
Q13315 ATM_HUMAN:

Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes.

Description:
ATM encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene.
Sequence:
MSLVLNDLLICCRQLEHDRATERKKEVEKFKRLIRDPETIKHLDRHSDSKQGKYLNWDAVFRFLQKYIQKETECLRIAKPNVSASTQASRQKKMQEISSLVKYFIKCANRRAPRLKCQELLNYIMDTVKDSSNGAIYGADCSNILLKDILSVRKYWCEISQQQWLELFSVYFRLYLKPSQDVHRVLVARIIHAVTKGCCSQTDGLNSKFLDFFSKAIQCARQEKSSSGLNHILAALTIFLKTLAVNFRIRVCELGDEILPTLLYIWTQHRLNDSLKEVIIELFQLQIYIHHPKGAKTQEKGAYESTKWRSILYNLYDLLVNEISHIGSRGKYSSGFRNIAVKENLIELMADICHQVFNEDTRSLEISQSYTTTQRESSDYSVPCKRKKIELGWEVIKDHLQKSQNDFDLVPWLQIATQLISKYPASLPNCELSPLLMILSQLLPQQRHGERTPYVLRCLTEVALCQDKRSNLESSQKSDLLKLWNKIWCITFRGISSEQIQAENFGLLGAIIQGSLVEVDREFWKLFTGSACRPSCPAVCCLTLALTTSIVPGTVKMGIEQNMCEVNRSFSLKESIMKWLLFYQLEGDLENSTEVPPILHSNFPHLVLEKILVSLTMKNCKAAMNFFQSVPECEHHQKDKEELSFSEVEELFLQTTFDKMDFLTIVRECGIEKHQSSIGFSVHQNLKESLDRCLLGLSEQLLNNYSSEITNSETLVRCSRLLVGVLGCYCYMGVIAEEEAYKSELFQKAKSLMQCAGESITLFKNKTNEEFRIGSLRNMMQLCTRCLSNCTKKSPNKIASGFFLRLLTSKLMNDIADICKSLASFIKKPFDRGEVESMEDDTNGNLMEVEDQSSMNLFNDYPDSSVSDANEPGESQSTIGAINPLAEEYLSKQDLLFLDMLKFLCLCVTTAQTNTVSFRAADIRRKLLMLIDSSTLEPTKSLHLHMYLMLLKELPGEEYPLPMEDVLELLKPLSNVCSLYRRDQDVCKTILNHVLHVVKNLGQSNMDSENTRDAQGQFLTVIGAFWHLTKERKYIFSVRMALVNCLKTLLEADPYSKWAILNVMGKDFPVNEVFTQFLADNHHQVRMLAAESINRLFQDTKGDSSRLLKALPLKLQQTAFENAYLKAQEGMREMSHSAENPETLDEIYNRKSVLLTLIAVVLSCSPICEKQALFALCKSVKENGLEPHLVKKVLEKVSETFGYRRLEDFMASHLDYLVLEWLNLQDTEYNLSSFPFILLNYTNIEDFYRSCYKVLIPHLVIRSHFDEVKSIANQIQEDWKSLLTDCFPKILVNILPYFAYEGTRDSGMAQQRETATKVYDMLKSENLLGKQIDHLFISNLPEIVVELLMTLHEPANSSASQSTDLCDFSGDLDPAPNPPHFPSHVIKATFAYISNCHKTKLKSILEILSKSPDSYQKILLAICEQAAETNNVYKKHRILKIYHLFVSLLLKDIKSGLGGAWAFVLRDVIYTLIHYINQRPSCIMDVSLRSFSLCCDLLSQVCQTAVTYCKDALENHLHVIVGTLIPLVYEQVEVQKQVLDLLKYLVIDNKDNENLYITIKLLDPFPDHVVFKDLRITQQKIKYSRGPFSLLEEINHFLSVSVYDALPLTRLEGLKDLRRQLELHKDQMVDIMRASQDNPQDGIMVKLVVNLLQLSKMAINHTGEKEVLEAVGSCLGEVGPIDFSTIAIQHSKDASYTKALKLFEDKELQWTFIMLTYLNNTLVEDCVKVRSAAVTCLKNILATKTGHSFWEIYKMTTDPMLAYLQPFRTSRKKFLEVPRFDKENPFEGLDDINLWIPLSENHDIWIKTLTCAFLDSGGTKCEILQLLKPMCEVKTDFCQTVLPYLIHDILLQDTNESWRNLLSTHVQGFFTSCLRHFSQTSRSTTPANLDSESEHFFRCCLDKKSQRTMLAVVDYMRRQKRPSSGTIFNDAFWLDLNYLEVAKVAQSCAAHFTALLYAEIYADKKSMDDQEKRSLAFEEGSQSTTISSLSEKSKEETGISLQDLLLEIYRSIGEPDSLYGCGGGKMLQPITRLRTYEHEAMWGKALVTYDLETAIPSSTRQAGIIQALQNLGLCHILSVYLKGLDYENKDWCPELEELHYQAAWRNMQWDHCTSVSKEVEGTSYHESLYNALQSLRDREFSTFYESLKYARVKEVEEMCKRSLESVYSLYPTLSRLQAIGELESIGELFSRSVTHRQLSEVYIKWQKHSQLLKDSDFSFQEPIMALRTVILEILMEKEMDNSQRECIKDILTKHLVELSILARTFKNTQLPERAIFQIKQYNSVSCGVSEWQLEEAQVFWAKKEQSLALSILKQMIKKLDASCAANNPSLKLTYTECLRVCGNWLAETCLENPAVIMQTYLEKAVEVAGNYDGESSDELRNGKMKAFLSLARFSDTQYQRIENYMKSSEFENKQALLKRAKEEVGLLREHKIQTNRYTVKVQRELELDELALRALKEDRKRFLCKAVENYINCLLSGEEHDMWVFRLCSLWLENSGVSEVNGMMKRDGMKIPTYKFLPLMYQLAARMGTKMMGGLGFHEVLNNLISRISMDHPHHTLFIILALANANRDEFLTKPEVARRSRITKNVPKQSSQLDEDRTEAANRIICTIRSRRPQMVRSVEALCDAYIILANLDATQWKTQRKGINIPADQPITKLKNLEDVVVPTMEIKVDHTGEYGNLVTIQSFKAEFRLAGGVNLPKIIDCVGSDGKERRQLVKGRDDLRQDAVMQQVFQMCNTLLQRNTETRKRKLTICTYKVVPLSQRSGVLEWCTGTVPIGEFLVNNEDGAHKRYRPNDFSAFQCQKKMMEVQKKSFEEKYEVFMDVCQNFQPVFRYFCMEKFLDPAIWFEKRLAYTRSVATSSIVGYILGLGDRHVQNILINEQSAELVHIDLGVAFEQGKILPTPETVPFRLTRDIVDGMGITGVEGVFRRCCEKTMEVMRNSQETLLTIVEVLLYDPLFDWTMNPLKALYLQQRPEDETELHPTLNADDQECKRNLSDIDQSFNKVAERVLMRLQEKLKGVEEGTVLSVGGQVNLLIQQAIDPKNLSRLFPGWKAWV

PTMs - Q13315 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
T39 Phosphorylation
K41 Ubiquitination
S47 Phosphorylation
S49 Phosphorylation
K53 Ubiquitination
Y54 Phosphorylation
K66 Ubiquitination
K70 Ubiquitination
T72 Phosphorylation
K79 Ubiquitination
S83 Phosphorylation
S85 Phosphorylation
T86 Phosphorylation
K106 Ubiquitination
K116 Ubiquitination
K147 Ubiquitination
K177 Ubiquitination
K196 Ubiquitination
S200 Phosphorylation
T202 Phosphorylation
K208 Ubiquitination
S214 Phosphorylation
K215 Ubiquitination
T237 Phosphorylation
T297 Phosphorylation
Y303 Phosphorylation
S305 Phosphorylation
K307 Ubiquitination
S310 Phosphorylation
Y313 Phosphorylation
K331 Ubiquitination
Y332 Phosphorylation
S367 Phosphorylation Q13315 (ATM)
Y370 Phosphorylation P00533 (EGFR)
T372 Phosphorylation
T373 Phosphorylation
Y380 Phosphorylation
S381 Phosphorylation
K385 Ubiquitination
K397 Ubiquitination
S440 Phosphorylation Q13315 (ATM) , Q13535 (ATR)
K468 Ubiquitination
K477 Ubiquitination
S569 Phosphorylation
K573 Ubiquitination
S644 Phosphorylation
S646 Phosphorylation
K673 Ubiquitination
K748 Ubiquitination
K750 Ubiquitination
K764 Ubiquitination
S794 Phosphorylation Q00535 (CDK5)
S800 Phosphorylation
S809 Phosphorylation
K810 Ubiquitination
K820 Ubiquitination
S821 Phosphorylation
K926 Ubiquitination
T935 Phosphorylation
Y980 Phosphorylation
K988 Ubiquitination
K1057 Ubiquitination
K1101 Ubiquitination
S1104 Phosphorylation
K1109 Ubiquitination
K1114 Ubiquitination
K1126 Methylation
K1126 Ubiquitination
K1181 Ubiquitination
K1196 Ubiquitination
K1269 Ubiquitination
K1280 Ubiquitination
K1317 Ubiquitination
K1323 Ubiquitination
K1398 Ubiquitination
S1403 Phosphorylation PR:Q96GD4 (hAURKB) , Q96GD4 (AURKB)
K1410 Ubiquitination
K1572 Ubiquitination
K1580 Ubiquitination
K1615 Ubiquitination
K1625 Ubiquitination
S1655 Phosphorylation
K1656 Methylation
K1656 Ubiquitination
T1662 Phosphorylation
K1665 Ubiquitination
S1673 Phosphorylation
K1692 Ubiquitination
K1698 Ubiquitination
K1701 Ubiquitination
Y1717 Phosphorylation
K1738 Ubiquitination
K1744 Ubiquitination
Y1753 Phosphorylation
Y1763 Phosphorylation
K1773 Ubiquitination
K1782 Ubiquitination
K1828 Ubiquitination
S1878 Phosphorylation
S1883 Phosphorylation
T1885 Phosphorylation Q13315 (ATM)
S1891 Phosphorylation
S1893 Phosphorylation Q13315 (ATM)
T1908 Phosphorylation
Y1915 Phosphorylation
S1974 Phosphorylation
S1981 Phosphorylation PR:Q96GD4 (hAURKB) , Q13535 (ATR) , Q13315 (ATM)
S1983 Phosphorylation
T1985 Phosphorylation
S1987 Phosphorylation Q13315 (ATM)
S1988 Phosphorylation
K1992 Ubiquitination
S1993 Phosphorylation
K1994 Ubiquitination
T1997 Phosphorylation
Y2019 Phosphorylation
K2025 Ubiquitination
T2031 Phosphorylation
K2089 Ubiquitination
K2148 Ubiquitination
K2153 Ubiquitination
K2160 Ubiquitination
S2162 Phosphorylation
S2165 Phosphorylation
S2168 Phosphorylation
Y2170 Phosphorylation
S2199 Phosphorylation
Y2202 Phosphorylation
K2207 Ubiquitination
K2248 Ubiquitination
K2266 Ubiquitination
K2303 Ubiquitination
S2310 Phosphorylation
K2318 Ubiquitination
K2406 Ubiquitination
K2413 Ubiquitination
K2421 Ubiquitination
K2431 Ubiquitination
K2440 Ubiquitination
K2505 Ubiquitination
K2515 Ubiquitination
K2589 Ubiquitination
S2592 Phosphorylation
K2643 Methylation
K2643 Ubiquitination
K2655 Ubiquitination
K2657 Ubiquitination
K2710 Ubiquitination
T2751 Phosphorylation
S2761 Phosphorylation Q13535 (ATR)
K2789 Ubiquitination
K2803 Ubiquitination
S2812 Phosphorylation
K2848 Ubiquitination
Y2969 Phosphorylation
K2992 Ubiquitination
S2996 Phosphorylation Q13315 (ATM)
K3004 Ubiquitination
K3016 Acetylation
K3053 Ubiquitination

PTMs - Q13315 As Enzyme

Substrate Site Source
O00255-2 (MEN1) S394 Uniprot
O14757 (CHEK1) S317 Uniprot
O14757 (CHEK1) S345 Uniprot
O15111 (CHUK) S473 Uniprot
O15151 (MDM4) S342 Uniprot
O15151 (MDM4) S367 Uniprot
O15151 (MDM4) S403 Uniprot
O15355 (PPM1G) S183 Uniprot
O15360 (FANCA) S1449 Uniprot
O43504 (LAMTOR5) S26 Uniprot
O43683 (BUB1) S314 Uniprot
O60934 (NBN) S278 Uniprot
O60934 (NBN) S343 Uniprot
O60934 (NBN) S397 Uniprot
O60934 (NBN) S615 Uniprot
O75150 (RNF40) S114 Uniprot
O75150 (RNF40) S819 Uniprot
O75150 (RNF40) S853 Uniprot
O75943-3 (RAD17) S470 Uniprot
O75943-3 (RAD17) S480 Uniprot
O75943-4 (RAD17) S549 Uniprot
O75943-4 (RAD17) S559 Uniprot
O75943-2 (RAD17) S635 Uniprot
O75943-2 (RAD17) S645 Uniprot
O75943 (RAD17) S646 Uniprot
O75943-1 (RAD17) S656 Uniprot
O94916 (NFAT5) S1197 Uniprot
O94916 (NFAT5) S1247 Uniprot
O94916 (NFAT5) S1367 Uniprot
O95071 (UBR5) S174 Uniprot
O95071 (UBR5) S191 Uniprot
O95071 (UBR5) S1532 Uniprot
O95071 (UBR5) S1679 Uniprot
O95071 (UBR5) S2586 Uniprot
O95863 (SNAI1) S100 Uniprot
O96017 (CHEK2) S19 Uniprot
O96017 (CHEK2) T26 Uniprot
O96017 (CHEK2) S28 Uniprot
O96017-12 (CHEK2) S33 Uniprot
O96017-1 (CHEK2) S35 Uniprot
O96017 (CHEK2) S50 Uniprot
O96017 (CHEK2) T68 Uniprot
O96017 (CHEK2) S357 Uniprot
O96028 (NSD2) S102 Uniprot
P00519 (ABL1) S446 Uniprot
P00519-2 (ABL1) S465 Uniprot
P04637-1 (TP53) S9 Uniprot
P04637 (TP53) S15 Uniprot
P04637-1 (TP53) S20 Uniprot
P04637-1 (TP53) S33 Uniprot
P04637-1 (TP53) S37 Uniprot
P04637 (TP53) S46 Uniprot
P08047 (SP1) S56 Uniprot
P08047 (SP1) S101 Uniprot
P12956 (XRCC6) S27 Uniprot
P12956 (XRCC6) S33 Uniprot
P15336 (ATF2) S490 Uniprot
P15336 (ATF2) S498 Uniprot
P15927 (RPA2) T21 Uniprot
P15927 (RPA2) S23 Uniprot
P15927 (RPA2) S29 Uniprot
P16104 (H2AFX) S140 Uniprot
P16220-2 (CREB1) S97 Uniprot
P16220 (CREB1) T100 Uniprot
P16220-2 (CREB1) S107 Uniprot
P16220 (CREB1) S111 Uniprot
P16220-1 (CREB1) S121 Uniprot
P16220 (CREB1) S133 Uniprot
P20823 (HNF1A) S249 Uniprot
P23025 (XPA) S173 Uniprot
P23025 (XPA) S196 Uniprot
P25205 (MCM3) S535 Uniprot
P25205 (MCM3) S728 Uniprot
P27707 (DCK) S74 Uniprot
P35637 (FUS) S42 Uniprot
P38398-3 (BRCA1) S284 Uniprot
P38398-6 (BRCA1) S320 Uniprot
P38398-3 (BRCA1) S353 Uniprot
P38398-3 (BRCA1) S420 Uniprot
P38398-3 (BRCA1) S438 Uniprot
P38398-8 (BRCA1) S1142 Uniprot
P38398-7 (BRCA1) S1189 Uniprot
P38398-8 (BRCA1) S1283 Uniprot
P38398-7 (BRCA1) S1330 Uniprot
P38398-8 (BRCA1) S1340 Uniprot
P38398-8 (BRCA1) S1376 Uniprot
P38398-7 (BRCA1) S1387 Uniprot
P38398-8 (BRCA1) S1410 Uniprot
P38398 (BRCA1) S1423 Uniprot
P38398-1 (BRCA1) S1457 Uniprot
P38398 (BRCA1) S1466 Uniprot
P38398-8 (BRCA1) S1477 Uniprot
P38398-8 (BRCA1) S1495 Uniprot
P38398 (BRCA1) S1497 Uniprot
P38398 (BRCA1) S1524 Uniprot
P38398-1 (BRCA1) S1542 Uniprot
P40692 (MLH1) S406 Uniprot
P41236 (PPP1R2) S44 Uniprot
P46379 (BAG6) S26 Uniprot
P46379 (BAG6) S218 Uniprot
P46527 (CDKN1B) S140 Uniprot
P49917 (LIG4) S199 Uniprot
P49959-2 (MRE11) S264 Uniprot
P49959 (MRE11) S676 Uniprot
P49959 (MRE11) S678 Uniprot
P50542 (PEX5) S141 Uniprot
P51532 (SMARCA4) S721 Uniprot
P54132 (BLM) T99 Uniprot
P54132 (BLM) T122 Uniprot
P54274 (TERF1) S219 Uniprot
P54274 (TERF1) S367 Uniprot
P55072 (VCP) S326 Uniprot
P55957 (BID) S78 Uniprot
P60484 (PTEN) S113 Uniprot
P60484 (PTEN) T398 Uniprot
P63151 (PPP2R2A) S400 Uniprot
P78527 (PRKDC) T2609 Uniprot
P78527 (PRKDC) S2612 Uniprot
P78527 (PRKDC) T2638 Uniprot
P78527 (PRKDC) T2647 Uniprot
Q00987 (MDM2) S386 Uniprot
Q00987 (MDM2) S395 Uniprot
Q00987-11 (MDM2) S401 Uniprot
Q00987 (MDM2) S429 Uniprot
Q01094 (E2F1) S31 Uniprot
Q01844 (EWSR1) T79 Uniprot
Q03111 (MLLT1) S394 Uniprot
Q03111 (MLLT1) S398 Uniprot
Q04206 (RELA) S547 Uniprot
Q07021 (C1QBP) S150 Uniprot
Q09472 (EP300) S106 Uniprot
Q12888 (TP53BP1) S6 Uniprot
Q12888-1 (TP53BP1) S25 Uniprot
Q12888 (TP53BP1) S29 Uniprot
Q12888 (TP53BP1) T302 Uniprot
Q12888-1 (TP53BP1) S784 Uniprot
Q12888 (TP53BP1) S831 Uniprot
Q12888 (TP53BP1) S1219 Uniprot
Q12888 (TP53BP1) S1778 Uniprot
Q13263 (TRIM28) S824 Uniprot
Q13315 (ATM) S367 Uniprot
Q13315 (ATM) S440 Uniprot
Q13315 (ATM) T1885 Uniprot
Q13315 (ATM) S1893 Uniprot
Q13315 (ATM) S1981 Uniprot
Q13315 (ATM) S1987 Uniprot
Q13315 (ATM) S2996 Uniprot
Q13362 (PPP2R5C) S520 Uniprot
Q13541 (EIF4EBP1) S8 Uniprot
Q13541 (EIF4EBP1) S94 Uniprot
Q13541 (EIF4EBP1) S112 Uniprot
Q14161 (GIT2) T195 Uniprot
Q14161 (GIT2) S384 Uniprot
Q14191 (WRN) S1141 Uniprot
Q14191 (WRN) S1292 Uniprot
Q14676 (MDC1) T4 Uniprot
Q14676 (MDC1) T98 Uniprot
Q14676 (MDC1) T719 Uniprot
Q14683 (SMC1A) S957 Uniprot
Q14683 (SMC1A) S966 Uniprot
Q14694 (USP10) T42 Uniprot
Q14694 (USP10) S337 Uniprot
Q14839 (CHD4) S1349 Uniprot
Q15648 (MED1) S816 Uniprot
Q15648 (MED1) S872 Uniprot
Q15648 (MED1) S874 Uniprot
Q15648 (MED1) S887 Uniprot
Q15648 (MED1) S953 Uniprot
Q15648 (MED1) S1134 Uniprot
Q15648 (MED1) S1141 Uniprot
Q15648 (MED1) S1147 Uniprot
Q15797 (SMAD1) S239 Uniprot
Q15831 (STK11) T363 Uniprot
Q15831 (STK11) T367 Uniprot
Q15910-2 (EZH2) S734 Uniprot
Q16204 (CCDC6) T434 Uniprot
Q16665 (HIF1A) S696 Uniprot
Q5TZA2-2 (CROCC) S922 Uniprot
Q5UIP0 (RIF1) S1851 Uniprot
Q5VTR2 (RNF20) S172 Uniprot
Q5VTR2 (RNF20) S522 Uniprot
Q5VTR2 (RNF20) S545 Uniprot
Q5XUX0 (FBXO31) S278 Uniprot
Q6PCD5 (RFWD3) S46 Uniprot
Q6PCD5 (RFWD3) S63 Uniprot
Q6UWZ7 (ABRAXAS1) S404 Uniprot
Q6UWZ7 (ABRAXAS1) S406 Uniprot
Q70CQ2 (USP34) S718 Uniprot
Q70CQ2 (USP34) S1751 Uniprot
Q7L7X3 (TAOK1) T643 Uniprot
Q7L7X3 (TAOK1) T785 Uniprot
Q7L7X3 (TAOK1) S990 Uniprot
Q7LG56 (RRM2B) S72 Uniprot
Q7Z6Z7 (HUWE1) S927 Uniprot
Q7Z6Z7 (HUWE1) S2339 Uniprot
Q7Z6Z7 (HUWE1) S2636 Uniprot
Q7Z6Z7 (HUWE1) S3377 Uniprot
Q7Z6Z7 (HUWE1) S3695 Uniprot
Q7Z6Z7 (HUWE1) S3797 Uniprot
Q86U86 (PBRM1) S948 Uniprot
Q8IUQ4 (SIAH1) S19 Uniprot
Q8IW19 (APLF) S116 Uniprot
Q8IY92 (SLX4) S1138 Uniprot
Q8IY92 (SLX4) S1271 Uniprot
Q8IY92 (SLX4) S1631 Uniprot
Q8IY92 (SLX4) S1716 Uniprot
Q8N0Z6 (TTC5) S203 Uniprot
Q8N680 (ZBTB2) S115 Uniprot
Q8NHY2 (COP1) S387 Uniprot
Q8TAQ2 (SMARCC2) S969 Uniprot
Q8TAQ5 (ZNF420) S68 Uniprot
Q92547 (TOPBP1) S492 Uniprot
Q92547 (TOPBP1) S554 Uniprot
Q92547 (TOPBP1) S853 Uniprot
Q92547 (TOPBP1) T1062 Uniprot
Q92547 (TOPBP1) S1138 Uniprot
Q92630 (DYRK2) T106 Uniprot
Q92630 (DYRK2) S442 Uniprot
Q92878 (RAD50) S635 Uniprot
Q92945 (KHSRP) S132 Uniprot
Q92945 (KHSRP) S274 Uniprot
Q92945 (KHSRP) S670 Uniprot
Q969H0 (FBXW7) S26 Uniprot
Q96J02-2 (ITCH) S161 Uniprot
Q96RL1 (UIMC1) S205 Uniprot
Q96RL1 (UIMC1) S402 Uniprot
Q96RU2 (USP28) S67 Uniprot
Q96RU2 (USP28) S495 Uniprot
Q96RU2 (USP28) S714 Uniprot
Q96RU2 (USP28) S720 Uniprot
Q96SD1-1 (DCLRE1C) T91 Uniprot
Q96SD1-2 (DCLRE1C) T131 Uniprot
Q96SD1-3 (DCLRE1C) T136 Uniprot
Q96SD1-1 (DCLRE1C) T251 Uniprot
Q96SD1-2 (DCLRE1C) S396 Uniprot
Q96SD1-3 (DCLRE1C) S401 Uniprot
Q96SD1-2 (DCLRE1C) S414 Uniprot
Q96SD1-2 (DCLRE1C) S418 Uniprot
Q96SD1-3 (DCLRE1C) S419 Uniprot
Q96SD1-3 (DCLRE1C) S423 Uniprot
Q96SD1-2 (DCLRE1C) S433 Uniprot
Q96SD1-3 (DCLRE1C) S438 Uniprot
Q96SD1-2 (DCLRE1C) S442 Uniprot
Q96SD1-3 (DCLRE1C) S447 Uniprot
Q96SD1 (DCLRE1C) S503 Uniprot
Q96SD1 (DCLRE1C) S516 Uniprot
Q96SD1-2 (DCLRE1C) S525 Uniprot
Q96SD1-3 (DCLRE1C) S530 Uniprot
Q96SD1-1 (DCLRE1C) S534 Uniprot
Q96SD1-1 (DCLRE1C) S538 Uniprot
Q96SD1-1 (DCLRE1C) S553 Uniprot
Q96SD1-1 (DCLRE1C) S562 Uniprot
Q96SD1 (DCLRE1C) S645 Uniprot
Q96T60 (PNKP) S114 Uniprot
Q96T60 (PNKP) S126 Uniprot
Q99504 (EYA3) S266 Uniprot
Q99638 (RAD9A) S272 Uniprot
Q99708-3 (RBBP8) S664 Uniprot
Q99708-3 (RBBP8) S745 Uniprot
Q99801 (NKX3-1) T134 Uniprot
Q99801 (NKX3-1) T166 Uniprot
Q9BQ15 (NABP2) T117 Uniprot
Q9BXS6 (NUSAP1) S124 Uniprot
Q9BXW9-1 (FANCD2) S222 Uniprot
Q9BXW9 (FANCD2) T691 Uniprot
Q9BXW9 (FANCD2) S717 Uniprot
Q9BXW9-1 (FANCD2) S1257 Uniprot
Q9BXW9 (FANCD2) S1401 Uniprot
Q9BXW9 (FANCD2) S1404 Uniprot
Q9BXW9 (FANCD2) S1407 Uniprot
Q9H2K8 (TAOK3) S324 Uniprot
Q9H2K8 (TAOK3) S359 Uniprot
Q9H2K8 (TAOK3) S551 Uniprot
Q9H3D4 (TP63) S477 Uniprot
Q9H3D4 (TP63) S479 Uniprot
Q9H3D4 (TP63) T491 Uniprot
Q9H3D4 (TP63) S560 Uniprot
Q9H9Q4 (NHEJ1) S251 Uniprot
Q9HB75 (PIDD1) T788 Uniprot
Q9NQS1 (AVEN) S135 Uniprot
Q9NQS1 (AVEN) S308 Uniprot
Q9NS23-2 (RASSF1) S131 Uniprot
Q9NS23 (RASSF1) S135 Uniprot
Q9NS91 (RAD18) S403 Uniprot
Q9NUW8 (TDP1) S81 Uniprot
Q9NUW8 (TDP1) S91 Uniprot
Q9NUW8 (TDP1) S365 Uniprot
Q9NUW8 (TDP1) S563 Uniprot
Q9NVI1 (FANCI) S556 Uniprot
Q9NVI1 (FANCI) S559 Uniprot
Q9NVI1 (FANCI) S730 Uniprot
Q9NVI1 (FANCI) S972 Uniprot
Q9NVI1 (FANCI) S1121 Uniprot
Q9NY61 (AATF) S189 Uniprot
Q9UBU7 (DBF4) T449 Uniprot
Q9UBU7 (DBF4) S502 Uniprot
Q9UBU7 (DBF4) S539 Uniprot
Q9UER7 (DAXX) S564 Uniprot
Q9UQE7 (SMC3) S1083 Uniprot
Q9UQR1 (ZNF148) S202 Uniprot
Q9Y2K7 (KDM2A) T632 Uniprot
Q9Y4E8 (USP15) S678 Uniprot
Q9Y6D9 (MAD1L1) S214 Uniprot
Q9Y6K9 (IKBKG) S85 Uniprot

Research Backgrounds

Function:

Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates DYRK2, CHEK2, p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9, UBQLN4 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends. Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation. Phosphorylates ATF2 which stimulates its function in DNA damage response. Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks.

PTMs:

Phosphorylated by NUAK1/ARK5. Autophosphorylation on Ser-367, Ser-1893, Ser-1981 correlates with DNA damage-mediated activation of the kinase. During the late stages of DNA damage response, dephosphorylated following deacetylation by SIRT7, leading to ATM deactivation.

Acetylation, on DNA damage, is required for activation of the kinase activity, dimer-monomer transition, and subsequent autophosphorylation on Ser-1981. Acetylated in vitro by KAT5/TIP60. Deacetylated by SIRT7 during the late stages of DNA damage response, promoting ATM dephosphorylation and subsequent deactivation.

Subcellular Location:

Nucleus. Cytoplasmic vesicle.
Note: Primarily nuclear. Found also in endocytic vesicles in association with beta-adaptin.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes.

Subunit Structure:

Homodimer. Dimers or tetramers in inactive state. On DNA damage, autophosphorylation dissociates ATM into monomers rendering them catalytically active. Binds p53/TP53, ABL1, BRCA1, NBN/nibrin and TERF1. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBN protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with RAD17; DNA damage promotes the association. Interacts with EEF1E1; the interaction, induced on DNA damage, up-regulates TP53. Interacts with DCLRE1C, KAT8, KAT5, NABP2, ATMIN and CEP164. Interacts with AP2B1 and AP3B2; the interaction occurs in cytoplasmic vesicles (By similarity). Interacts with TELO2 and TTI1. Interacts with DDX1. Interacts with BRAT1.

Family&Domains:

The FATC domain is required for interaction with KAT5.

Belongs to the PI3/PI4-kinase family. ATM subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Genetic Information Processing > Replication and repair > Homologous recombination.

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

References

1). ITGB1 enhances the Radioresistance of human Non-small Cell Lung Cancer Cells by modulating the DNA damage response and YAP1-induced Epithelial-mesenchymal Transition. International Journal of Biological Sciences, 2023 (PubMed: 33613118) [IF=9.2]

Application: WB    Species: human    Sample: NSCLC cells

Figure 7.| ITGB1 expression correlates with the expression of proteins related to the ATM/CHK2/CDC25c pathway. A. The relationship between ITGB1 expression and that of 34 genes in the DNA-DSB response pathways. B-D. Protein levels of ATM (p-ATM), CHK2 (p-CHK2), and CDC25c (p-CDC25c) were detected by western blotting in shITGB1 and ITGB1 overexpression groups.

Application: WB    Species: Human    Sample: NSCLC cells

Figure 7 ITGB1 expression correlates with the expression of proteins related to the ATM/CHK2/CDC25c pathway. A. The relationship between ITGB1 expression and that of 34 genes in the DNA-DSB response pathways. B-D. Protein levels of ATM (p-ATM), CHK2 (p-CHK2), and CDC25c (p-CDC25c) were detected by western blotting in shITGB1 and ITGB1 overexpression groups.
2). EBV encoded miRNA BART8-3p promotes radioresistance in nasopharyngeal carcinoma by regulating ATM/ATR signaling pathway. BIOSCIENCE REPORTS, 2019 (PubMed: 31471531) [IF=4.0]

Application: WB    Species: mouse    Sample: EBV-negative NPC cell

Figure 5. |EBV-miR-BART8-3p activates ATM/ATR. (A) Western blot analysis of p-ATM/ATM, p-ATR/ATR, and p-DNA-PKcs/DNA-PKcs in response to 0–12 h of irradiation at 2 Gy in the two groups.

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