Twist2 Antibody - #AF4112

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Product: Twist2 Antibody
Catalog: AF4112
Description: Rabbit polyclonal antibody to Twist2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 18kd; 18kD(Calculated).
Uniprot: Q8WVJ9
RRID: AB_2835360

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(92%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Twist2 Antibody detects endogenous levels of total Twist2.
RRID:
AB_2835360
Cite Format: Affinity Biosciences Cat# AF4112, RRID:AB_2835360.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

bHLHa39; Class A basic helix-loop-helix protein 39; Dermis expressed protein 1; Dermis-expressed protein 1; DERMO 1; Dermo-1; DERMO1; MGC117334; Twist 2; Twist homolog 2 (Drosophila); Twist homolog 2; Twist related bHLH protein Dermo1; Twist related protein 2; Twist-related protein 2; Twist2; TWST2_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q8WVJ9(TWST2_HUMAN) >>Visit HPA database.

Gene(ID):
TWIST2(117581)
Expression:
Q8WVJ9 TWST2_HUMAN:

In the embryo, highly expressed in chondrogenic cells. In embryonic skin, expressed in the undifferentiated mesenchymal layer beneath the epidermis which later develops into the dermis. Expressed in early myeloid cells but not in lymphoid cells in the liver. Expression also detected in the secretory ependymal epithelium of the choroid plexus primordium. In the adult, expressed in secreting glandular tissues and tubules.

Description:
Binds to the E-box consensus sequence 5'-CANNTG-3' as a heterodimer and inhibits transcriptional activation by MYOD1, MYOG, MEF2A and MEF2C. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Involved in postnatal glycogen storage and energy metabolism. Inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors.
Sequence:
MEEGSSSPVSPVDSLGTSEEELERQPKRFGRKRRYSKKSSEDGSPTPGKRGKKGSPSAQSFEELQSQRILANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDEMDNKMTSCSYVAHERLSYAFSVWRMEGAWSMSASH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
92
Zebrafish
63
Horse
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8WVJ9 As Substrate

Site PTM Type Enzyme
S55 Phosphorylation
S60 Phosphorylation
S66 Phosphorylation
T95 Phosphorylation
K100 Ubiquitination
S102 Phosphorylation
K103 Ubiquitination

Research Backgrounds

Function:

Binds to the E-box consensus sequence 5'-CANNTG-3' as a heterodimer and inhibits transcriptional activation by MYOD1, MYOG, MEF2A and MEF2C. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Involved in postnatal glycogen storage and energy metabolism (By similarity). Inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Mainly nuclear during embryonic development. Cytoplasmic in adult tissues.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

In the embryo, highly expressed in chondrogenic cells. In embryonic skin, expressed in the undifferentiated mesenchymal layer beneath the epidermis which later develops into the dermis. Expressed in early myeloid cells but not in lymphoid cells in the liver. Expression also detected in the secretory ependymal epithelium of the choroid plexus primordium. In the adult, expressed in secreting glandular tissues and tubules.

Subunit Structure:

Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with TCF3/E12. Also interacts with MEF2C (By similarity).

Research Fields

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

References

1). Study on the Potential Mechanism of miR-22-5p in Non-Small-Cell Lung Cancer. DISEASE MARKERS, 2022 (PubMed: 36111260)

Application: WB    Species: Human    Sample: NCI-H1299 cells and A549 cells

Figure 3 TWISTt2 is a direct target of miR-22-5p. (a) Schematic of the reporter construct of the wild-type and mutated TWIST2 3′-UTR sequences. (b) Luciferase reporter assay of the direct targeting activity of miR-22-5p to the TWIST2 3′-UTR by cotransfection with miR-22-5p mimics and wt/mut TWIST2 luciferase reporter vectors. wt: wild type; mut: mutated. (c) qRT-PCR analysis of the mRNA levels of TWIST2 regulated by miR-22-5p in NCI-H1299 and A549 cells. (d) Western blot analysis of the protein expression levels of TWIST2 regulated by miR-22-5p in NSCLC cells

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