Nanog Antibody - #AF4011
| Product: | Nanog Antibody |
| Catalog: | AF4011 |
| Description: | Rabbit polyclonal antibody to Nanog |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Prediction: | Rat, Bovine, Horse, Sheep, Rabbit |
| Mol.Wt.: | 42kd; 35kD(Calculated). |
| Uniprot: | Q9H9S0 |
| RRID: | AB_2835339 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF4011, RRID:AB_2835339.
Fold/Unfold
Embryonic stem cell specific homeobox protein (Nanog); ENK; FLJ12581; hNanog; Homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; NANOG; Nanog homeobox; NANOG_HUMAN;
Immunogens
Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
- Q9H9S0 NANOG_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSVDPACPQSLPCFEASDCKESSPMPVICGPEENYPSLQMSSAEMPHTETVSPLPSSMDLLIQDSPDSSTSPKGKQPTSAEKSVAKKEDKVPVKKQKTRTVFSSTQLCVLNDRFQRQKYLSLQQMQELSNILNLSYKQVKTWFQNQRMKSKRWQKNNWPKNSNGVTQKASAPTYPSLYSSYHQGCLVNPTGNLPMWSNQTWNNSTWSNQTQNIQSWSNHSWNTQTWCTQSWNNQAWNSPFYNCGEESLQSCMQFQPNSPASDLEAALEAAGEGLNVIQQTTRYFSTPQTMDLFLNYSMNMQPEDV
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9H9S0 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| Y35 | Phosphorylation | Q05397 (PTK2) | Uniprot |
| T50 | Phosphorylation | Uniprot | |
| S52 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S56 | Phosphorylation | Uniprot | |
| S57 | Phosphorylation | Uniprot | |
| S65 | Phosphorylation | Uniprot | |
| T70 | Phosphorylation | Uniprot | |
| S71 | Phosphorylation | P28482 (MAPK1) , P06493 (CDK1) | Uniprot |
| T78 | Phosphorylation | Q02156 (PRKCE) , P28482 (MAPK1) | Uniprot |
| S79 | Phosphorylation | Q02156 (PRKCE) | Uniprot |
| S135 | Phosphorylation | Q02156 (PRKCE) | Uniprot |
| Y174 | Phosphorylation | Q05397 (PTK2) | Uniprot |
| T200 | Phosphorylation | Q02156 (PRKCE) | Uniprot |
| S258 | Phosphorylation | Uniprot | |
| T280 | Phosphorylation | Q02156 (PRKCE) | Uniprot |
| T286 | Phosphorylation | Uniprot | |
| T289 | Phosphorylation | Uniprot |
Research Backgrounds
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
Nucleus.
Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
Interacts with SMAD1 and SALL4. Interacts with ZNF281/ZFP281 (By similarity). Interacts with PCGF1. Interacts with ESRRB; reciprocally modulates their transcriptional activities (By similarity).
Belongs to the Nanog homeobox family.
Research Fields
· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
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