Phospho-ROCK2 (Tyr722) Antibody - #AF3028

(1)   (14)  
Product: Phospho-ROCK2 (Tyr722) Antibody
Catalog: AF3028
Description: Rabbit polyclonal antibody to Phospho-ROCK2 (Tyr722)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 165kDa; 161kD(Calculated).
Uniprot: O75116
RRID: AB_2835336

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
Phospho-ROCK2 (Tyr722) Antibody detects endogenous levels of ROCK2 only when phosphorylated at Tyr722.
RRID:
AB_2835336
Cite Format: Affinity Biosciences Cat# AF3028, RRID:AB_2835336.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

coiled-coil-containing protein kinase 2; KIAA0619; p164 ROCK 2; p164 ROCK-2; Rho associated coiled coil containing protein kinase 2; Rho associated protein kinase 2; Rho associated, coiled coil containing protein kinase II; Rho kinase 2; Rho-associated; Rho-associated protein kinase 2; ROCK 2; Rock II; Rock2; ROCK2_HUMAN; Rock2m; ROK alpha; ROKalpha;

Immunogens

Immunogen:
Uniprot:

O75116(ROCK2_HUMAN) >>Visit HPA database.

Gene(ID):
ROCK2(9475)
Expression:
O75116 ROCK2_HUMAN:

Expressed in the brain (at protein level).

Description:
The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq]
Sequence:
MSRPPPTGKMPGAPETAPGDGAGASRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGFYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIRQHPFFKNDQWHWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCRETDSIQSRKNEESQEIQKKLYTLEEHLSNEMQAKEELEQKCKSVNTRLEKTAKELEEEITLRKSVESALRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQISTEKVNQLQRQLDETNALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESERRDRTHGSEIINDLQGRICGLEEDLKNGKILLAKVELEKRQLQERFTDLEKEKSNMEIDMTYQLKVIQQSLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERTLKQKVENLLLEAEKRCSLLDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLMEMKMNLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLGKELQQKKQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEKEELNNKLKDVQEQLSRLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGNDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGLDSSSIGSGPGDAEADDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
91
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75116 As Substrate

Site PTM Type Enzyme
T16 Phosphorylation
S25 Phosphorylation
K29 Ubiquitination
Y92 Phosphorylation
K96 Ubiquitination
K121 Ubiquitination
K125 Ubiquitination
K216 Ubiquitination
T249 Phosphorylation
S298 Phosphorylation
K323 Ubiquitination
K355 Ubiquitination
K391 Ubiquitination
T396 Phosphorylation
T414 Phosphorylation O75116 (ROCK2)
S423 Phosphorylation
S425 Phosphorylation
K448 Ubiquitination
K482 Ubiquitination
K492 Ubiquitination
K505 Ubiquitination
K511 Ubiquitination
K537 Ubiquitination
K556 Ubiquitination
T567 Phosphorylation
T573 Phosphorylation
S575 Phosphorylation
T577 Phosphorylation
K590 Ubiquitination
K605 Ubiquitination
K618 Ubiquitination
K669 Ubiquitination
K709 Ubiquitination
K720 Ubiquitination
Y722 Phosphorylation P12931 (SRC)
S724 Phosphorylation
K729 Ubiquitination
K749 Ubiquitination
K759 Ubiquitination
K795 Ubiquitination
K860 Ubiquitination
K877 Ubiquitination
S930 Phosphorylation
Y936 Phosphorylation
S937 Phosphorylation
K946 Acetylation
K951 Ubiquitination
K964 Ubiquitination
T967 Phosphorylation P53350 (PLK1)
K989 Ubiquitination
K995 Ubiquitination
K997 Ubiquitination
K1007 Ubiquitination
K1017 Ubiquitination
K1022 Ubiquitination
K1071 Ubiquitination
T1078 Phosphorylation
K1083 Ubiquitination
K1086 Ubiquitination
S1099 Phosphorylation P53350 (PLK1)
S1114 Phosphorylation
S1121 Phosphorylation
S1132 Phosphorylation
S1133 Phosphorylation P53350 (PLK1)
S1134 Phosphorylation
S1137 Phosphorylation
T1165 Phosphorylation
K1181 Ubiquitination
S1187 Phosphorylation
S1194 Phosphorylation
Y1197 Phosphorylation
T1212 Phosphorylation
K1222 Ubiquitination
Y1232 Phosphorylation
S1238 Phosphorylation
K1252 Ubiquitination
K1316 Ubiquitination
Y1318 Phosphorylation
Y1319 Phosphorylation
T1334 Phosphorylation
K1339 Ubiquitination
K1350 Acetylation
K1351 Acetylation
K1351 Ubiquitination
S1361 Phosphorylation
S1362 Phosphorylation
T1365 Phosphorylation
S1366 Phosphorylation
K1368 Methylation
K1368 Ubiquitination
S1374 Phosphorylation P53350 (PLK1)
S1379 Phosphorylation
S1388 Phosphorylation

PTMs - O75116 As Enzyme

Substrate Site Source
O14974 (PPP1R12A) T443 Uniprot
O14974 (PPP1R12A) T696 Uniprot
O14974 (PPP1R12A) S852 Uniprot
O14974-1 (PPP1R12A) T853 Uniprot
O75116 (ROCK2) T414 Uniprot
P05067-4 (APP) T654 Uniprot
P08670 (VIM) S39 Uniprot
P08670 (VIM) S72 Uniprot
P15311 (EZR) T567 Uniprot
P19634 (SLC9A1) T653 Uniprot
P24844 (MYL9) S20 Uniprot
P26038 (MSN) T558 Uniprot
P29474 (NOS3) T495 Uniprot
P35241 (RDX) T564 Uniprot
P35568 (IRS1) S636 Uniprot
P35568 (IRS1) S974 Uniprot
P35611 (ADD1) T445 Uniprot
P35611 (ADD1) T480 Uniprot
P51911 (CNN1) T170 Uniprot
P51911 (CNN1) S175 Uniprot
P51911 (CNN1) T180 Uniprot
P51911 (CNN1) T184 Uniprot
P51911 (CNN1) T259 Uniprot
P53671 (LIMK2) T505 Uniprot
P56817 (BACE1) S498 Uniprot
P68104 (EEF1A1) T432 Uniprot
Q15306 (IRF4) S447 Uniprot
Q15306 (IRF4) S448 Uniprot
Q15417 (CNN3) S293 Uniprot
Q16555 (DPYSL2) T555 Uniprot
Q92673 (SORL1) S2206 Uniprot
Q96A00 (PPP1R14A) T38 Uniprot
Q96C90 (PPP1R14B) T57 Uniprot
Q99962 (SH3GL2) T14 Uniprot

Research Backgrounds

Function:

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.

PTMs:

Phosphorylation at Tyr-722 reduces its binding to RHOA and is crucial for focal adhesion dynamics. Dephosphorylation by PTPN11 stimulates its RHOA binding activity.

Cleaved by granzyme B during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.

Subcellular Location:

Cytoplasm. Cell membrane>Peripheral membrane protein. Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Cytoplasmic, and associated with actin microfilaments and the plasma membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in the brain (at protein level).

Subunit Structure:

Homodimer (By similarity). Interacts with IRS1 (By similarity). Interacts with RAF1 (By similarity). Interacts with RHOA (activated by GTP), RHOB and RHOC (By similarity). Interacts with PPP1R12A. Interacts with EP300. Interacts with CHORDC1. Interacts with BRCA2. Interacts with NPM1; this interaction enhances ROCK2 activity. Interacts with SORL1.

Family&Domains:

An interaction between Thr-414 and Asp-48 is essential for kinase activity and dimerization.

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

References

1). Baicalin Attenuates Panton-Valentine Leukocidin (PVL)-Induced Cytoskeleton Rearrangement via Regulating the RhoA/ROCK/LIMK and PI3K/AKT/GSK-3β Pathways in Bovine Mammary Epithelial Cells. International journal of molecular sciences, 2023 (PubMed: 37833969) [IF=5.6]

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.