STAT4 Mouse Monoclonal Antibody | Affinity Biosciences

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Western blot analysis of extracts from various samples, using STAT4 Mouse Monoclonal Antibody.

Product:	STAT4 Mouse Monoclonal Antibody
Catalog:	BF8180
Description:	Mouse monoclonal antibody to STAT4
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Horse, Rabbit, Chicken
Mol.Wt.:	86kDa; 86kD(Calculated).
Uniprot:	Q14765

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Monoclonal [AFfirm8180]

Specificity:

STAT4 Antibody detects endogenous levels of total STAT4.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Signal transducer and activator of transcription 4; SLEB11; STAT4; STAT4_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q14765(STAT4_HUMAN) >>Visit HPA database.

Gene(ID):

STAT4(6775)

Description:

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators.

Sequence:

Q14765 STAT4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSQWNQVQQLEIKFLEQVDQFYDDNFPMEIRHLLAQWIENQDWEAASNNETMATILLQNLLIQLDEQLGRVSKEKNLLLIHNLKRIRKVLQGKFHGNPMHVAVVISNCLREERRILAAANMPVQGPLEKSLQSSSVSERQRNVEHKVAAIKNSVQMTEQDTKYLEDLQDEFDYRYKTIQTMDQSDKNSAMVNQEVLTLQEMLNSLDFKRKEALSKMTQIIHETDLLMNTMLIEELQDWKRRQQIACIGGPLHNGLDQLQNCFTLLAESLFQLRRQLEKLEEQSTKMTYEGDPIPMQRTHMLERVTFLIYNLFKNSFVVERQPCMPTHPQRPLVLKTLIQFTVKLRLLIKLPELNYQVKVKASIDKNVSTLSNRRFVLCGTNVKAMSIEESSNGSLSVEFRHLQPKEMKSSAGGKGNEGCHMVTEELHSITFETQICLYGLTIDLETSSLPVVMISNVSQLPNAWASIIWYNVSTNDSQNLVFFNNPPPATLSQLLEVMSWQFSSYVGRGLNSDQLHMLAEKLTVQSSYSDGHLTWAKFCKEHLPGKSFTFWTWLEAILDLIKKHILPLWIDGYVMGFVSKEKERLLLKDKMPGTFLLRFSESHLGGITFTWVDHSESGEVRFHSVEPYNKGRLSALPFADILRDYKVIMAENIPENPLKYLYPDIPKDKAFGKHYSSQPCEVSRPTERGDKGYVPSVFIPISTIRSDSTEPHSPSDLLPMSPSVYAVLRENLSPTTIETAMKSPYSAE

PTMs - Q14765 As Substrate

Q14765

Site	PTM Type	Enzyme	Source
S130	Phosphorylation		Uniprot
S137	Phosphorylation		Uniprot
K151	Ubiquitination		Uniprot
T284	Phosphorylation		Uniprot
T287	Phosphorylation		Uniprot
Y288	Phosphorylation		Uniprot
T298	Phosphorylation		Uniprot
S362	Phosphorylation		Uniprot
T369	Phosphorylation		Uniprot
S371	Phosphorylation		Uniprot
S386	Phosphorylation		Uniprot
S394	Phosphorylation		Uniprot
S396	Phosphorylation		Uniprot
K562	Ubiquitination		Uniprot
Y645	Phosphorylation		Uniprot
Y693	Phosphorylation	O60674 (JAK2)	Uniprot
S713	Phosphorylation		Uniprot
S721	Phosphorylation	Q16539 (MAPK14) , P52564 (MAP2K6)	Uniprot
S733	Phosphorylation		Uniprot
S743	Phosphorylation		Uniprot
Y745	Phosphorylation		Uniprot
S746	Phosphorylation		Uniprot

Research Backgrounds

Q14765_STAT4_HUMAN

Function:

Carries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling.

PTMs:

Tyrosine phosphorylated. Serine phosphorylation is also required for maximal transcriptional activity (By similarity).

Subcellular Location:

Cytoplasm. Nucleus.
Note: Translocated into the nucleus in response to phosphorylation.

Subunit Structure:

Forms a homodimer or a heterodimer with a related family member. Interacts with ARL2BP (By similarity). The SH2 domain interacts, in vitro, with IL12RB2 via a short cytoplasmic domain.

Family&Domains:

Belongs to the transcription factor STAT family.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)

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AFfirm™ STAT4 Mouse Monoclonal Antibody - #BF8180