PLCG1 Mouse Monoclonal Antibody | Affinity Biosciences

Product:	PLCG1 Mouse Monoclonal Antibody
Catalog:	BF8162
Description:	Mouse monoclonal antibody to PLCG1
Application:	WB
Reactivity:	Human
Prediction:	Pig, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	150kDa; 149kD(Calculated).
Uniprot:	P19174

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Monoclonal [AFfirm8162]

Specificity:

PLCG1 Mouse Monoclonal Antibody detects endogenous levels of total PLCG1.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

1 phosphatidyl D myo inositol 4 5 bisphosphate; 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1; Inositoltrisphosphohydrolase; Monophosphatidylinositol phosphodiesterase; NCKAP3; Phosphatidylinositol phospholipase C; Phosphoinositidase C; Phosphoinositide phospholipase C; Phosphoinositide phospholipase C-gamma-1; Phospholipase C 148; Phospholipase C gamma 1; Phospholipase C-gamma-1; Phospholipase C-II; PLC gamma 1; PLC II; PLC-148; PLC-gamma-1; PLC-II; PLC1; PLC148; Plcg1; PLCG1_HUMAN; PLCgamma1;

Immunogens

Immunogen:

Uniprot:

P19174(PLCG1_HUMAN) >>Visit HPA database.

Gene(ID):

PLCG1(5335)

Description:

The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.

Sequence:

P19174 PLCG1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAGAASPCANGCGPGAPSDAEVLHLCRSLEVGTVMTLFYSKKSQRPERKTFQVKLETRQITWSRGADKIEGAIDIREIKEIRPGKTSRDFDRYQEDPAFRPDQSHCFVILYGMEFRLKTLSLQATSEDEVNMWIKGLTWLMEDTLQAPTPLQIERWLRKQFYSVDRNREDRISAKDLKNMLSQVNYRVPNMRFLRERLTDLEQRSGDITYGQFAQLYRSLMYSAQKTMDLPFLEASTLRAGERPELCRVSLPEFQQFLLDYQGELWAVDRLQVQEFMLSFLRDPLREIEEPYFFLDEFVTFLFSKENSVWNSQLDAVCPDTMNNPLSHYWISSSHNTYLTGDQFSSESSLEAYARCLRMGCRCIELDCWDGPDGMPVIYHGHTLTTKIKFSDVLHTIKEHAFVASEYPVILSIEDHCSIAQQRNMAQYFKKVLGDTLLTKPVEISADGLPSPNQLKRKILIKHKKLAEGSAYEEVPTSMMYSENDISNSIKNGILYLEDPVNHEWYPHYFVLTSSKIYYSEETSSDQGNEDEEEPKEVSSSTELHSNEKWFHGKLGAGRDGRHIAERLLTEYCIETGAPDGSFLVRESETFVGDYTLSFWRNGKVQHCRIHSRQDAGTPKFFLTDNLVFDSLYDLITHYQQVPLRCNEFEMRLSEPVPQTNAHESKEWYHASLTRAQAEHMLMRVPRDGAFLVRKRNEPNSYAISFRAEGKIKHCRVQQEGQTVMLGNSEFDSLVDLISYYEKHPLYRKMKLRYPINEEALEKIGTAEPDYGALYEGRNPGFYVEANPMPTFKCAVKALFDYKAQREDELTFIKSAIIQNVEKQEGGWWRGDYGGKKQLWFPSNYVEEMVNPVALEPEREHLDENSPLGDLLRGVLDVPACQIAIRPEGKNNRLFVFSISMASVAHWSLDVAADSQEELQDWVKKIREVAQTADARLTEGKIMERRKKIALELSELVVYCRPVPFDEEKIGTERACYRDMSSFPETKAEKYVNKAKGKKFLQYNRLQLSRIYPKGQRLDSSNYDPLPMWICGSQLVALNFQTPDKPMQMNQALFMTGRHCGYVLQPSTMRDEAFDPFDKSSLRGLEPCAISIEVLGARHLPKNGRGIVCPFVEIEVAGAEYDSTKQKTEFVVDNGLNPVWPAKPFHFQISNPEFAFLRFVVYEEDMFSDQNFLAQATFPVKGLKTGYRAVPLKNNYSEDLELASLLIKIDIFPAKENGDLSPFSGTSLRERGSDASGQLFHGRAREGSFESRYQQPFEDFRISQEHLADHFDSRERRAPRRTRVNGDNRL

PTMs - P19174 As Substrate

P19174

Site	PTM Type	Enzyme	Source
	Phosphorylation		Uniprot
A2	Acetylation		Uniprot
S6	Phosphorylation		Uniprot
K54	Ubiquitination		Uniprot
K68	Ubiquitination		Uniprot
Y93	Phosphorylation		Uniprot
K159	Ubiquitination		Uniprot
K175	Ubiquitination		Uniprot
K178	Ubiquitination		Uniprot
Y186	Phosphorylation		Uniprot
Y210	Phosphorylation		Uniprot
S219	Phosphorylation		Uniprot
K226	Ubiquitination		Uniprot
S236	Phosphorylation		Uniprot
T237	Phosphorylation		Uniprot
Y379	Phosphorylation		Uniprot
K389	Ubiquitination		Uniprot
S412	Phosphorylation		Uniprot
Y428	Phosphorylation		Uniprot
K431	Ubiquitination		Uniprot
K440	Ubiquitination		Uniprot
S451	Phosphorylation		Uniprot
K456	Ubiquitination		Uniprot
K465	Ubiquitination		Uniprot
S470	Phosphorylation		Uniprot
Y472	Phosphorylation	P00533 (EGFR)	Uniprot
T477	Phosphorylation		Uniprot
Y481	Phosphorylation		Uniprot
Y496	Phosphorylation		Uniprot
Y506	Phosphorylation		Uniprot
Y509	Phosphorylation		Uniprot
Y518	Phosphorylation		Uniprot
S525	Phosphorylation		Uniprot
S539	Phosphorylation		Uniprot
T542	Phosphorylation		Uniprot
K549	Ubiquitination		Uniprot
K554	Ubiquitination		Uniprot
S582	Phosphorylation		Uniprot
K666	Ubiquitination		Uniprot
Y669	Phosphorylation		Uniprot
Y702	Phosphorylation		Uniprot
K763	Ubiquitination		Uniprot
T766	Phosphorylation		Uniprot
Y771	Phosphorylation	P43405 (SYK) , P00533 (EGFR) , P00519 (ABL1) , P07948 (LYN)	Uniprot
Y775	Phosphorylation	Q08881 (ITK)	Uniprot
Y783	Phosphorylation	P43403 (ZAP70) , P43405 (SYK) , Q08881 (ITK) , P07949 (RET) , P00533 (EGFR) , P54764 (EPHA4)	Uniprot
T791	Phosphorylation		Uniprot
K793	Ubiquitination		Uniprot
K803	Ubiquitination		Uniprot
S815	Phosphorylation		Uniprot
K823	Ubiquitination		Uniprot
Y833	Phosphorylation		Uniprot
Y845	Phosphorylation		Uniprot
K890	Ubiquitination		Uniprot
K941	Ubiquitination		Uniprot
S954	Phosphorylation		Uniprot
Y959	Phosphorylation		Uniprot
Y977	Phosphorylation		Uniprot
S981	Phosphorylation		Uniprot
S982	Phosphorylation		Uniprot
Y991	Phosphorylation		Uniprot
Y1003	Phosphorylation	P00519 (ABL1)	Uniprot
C1060	S-Nitrosylation		Uniprot
K1079	Ubiquitination		Uniprot
K1193	Ubiquitination		Uniprot
S1221	Phosphorylation		Uniprot
S1224	Phosphorylation		Uniprot
T1226	Phosphorylation		Uniprot
S1227	Phosphorylation		Uniprot
R1231	Methylation		Uniprot
S1233	Phosphorylation		Uniprot
S1236	Phosphorylation		Uniprot
R1243	Methylation		Uniprot
S1248	Phosphorylation	P17252 (PRKCA) , P17612 (PRKACA) , P31749 (AKT1) , Q15139 (PRKD1)	Uniprot
S1251	Phosphorylation		Uniprot
Y1253	Phosphorylation	P43403 (ZAP70) , P00533 (EGFR)	Uniprot
S1263	Phosphorylation		Uniprot
S1273	Phosphorylation		Uniprot
R1283	Methylation		Uniprot

Research Backgrounds

P19174_PLCG1_HUMAN

Function:

Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration.

PTMs:

Tyrosine phosphorylated in response to signaling via activated FLT3, KIT and PDGFRA (By similarity). Tyrosine phosphorylated by activated FGFR1, FGFR2, FGFR3 and FGFR4. Tyrosine phosphorylated by activated FLT1 and KDR. Tyrosine phosphorylated by activated PDGFRB. The receptor-mediated activation of PLCG1 involves its phosphorylation by tyrosine kinases, in response to ligation of a variety of growth factor receptors and immune system receptors. For instance, SYK phosphorylates and activates PLCG1 in response to ligation of the B-cell receptor. May be dephosphorylated by PTPRJ. Phosphorylated by ITK and TXK on Tyr-783 upon TCR activation in T-cells.

Ubiquitinated by CBLB in activated T-cells.

Subcellular Location:

Cell projection>Lamellipodium. Cell projection>Ruffle.
Note: Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.

Subunit Structure:

Interacts with AGAP2 via its SH3 domain. Interacts (via SH2 domain) with RET. Interacts with FLT1 (tyrosine-phosphorylated) (By similarity). Interacts (via SH2 domain) with FGFR1, FGFR2, FGFR3 and FGFR4 (phosphorylated). Interacts with LAT (phosphorylated) upon TCR activation. Interacts (via SH3 domain) with the Pro-rich domain of TNK1. Associates with BLNK, VAV1, GRB2 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with CBLB in activated T-cells; which inhibits phosphorylation. Interacts with SHB. Interacts (via SH3 domain) with the Arg/Gly-rich-flanked Pro-rich domains of KHDRBS1/SAM68. This interaction is selectively regulated by arginine methylation of KHDRBS1/SAM68. Interacts with INPP5D/SHIP1, THEMIS and CLNK (By similarity). Interacts with AXL, FLT4 and KIT. Interacts with RALGPS1. Interacts (via the SH2 domains) with VIL1 (phosphorylated at C-terminus tyrosine phosphorylation sites). Interacts (via SH2 domain) with PDGFRA and PDGFRB (tyrosine phosphorylated). Interacts with PIP5K1C (By similarity). Interacts with NTRK1 and NTRK2 (phosphorylated upon ligand-binding). Interacts with SYK; activates PLCG1. Interacts with GRB2, LAT and THEMIS upon TCR activation in thymocytes (By similarity). Interacts with TESPA1; the association is increased with prolonged stimulation of the TCR and may facilitate the assembly of the LAT signalosome.

(Microbial infection) Interacts (via SH3 domain) with HEV ORF3 protein.

Family&Domains:

The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.

Research Fields

· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Glioma. (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)

· Human Diseases > Cancers: Overview > Choline metabolism in cancer. (View pathway)

· Metabolism > Carbohydrate metabolism > Inositol phosphate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Development > Axon guidance. (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)

· Organismal Systems > Immune system > Fc epsilon RI signaling pathway. (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels. (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)

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AFfirm™ PLCG1 Mouse Monoclonal Antibody - #BF8162