VEGFB Antibody - #AF7019

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Product: VEGFB Antibody
Catalog: AF7019
Description: Rabbit polyclonal antibody to VEGFB
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep
Mol.Wt.: 17,21kDa; 22kD(Calculated).
Uniprot: P49765
RRID: AB_2835323

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 50ul $250 In stock
 100ul $350 In stock
 200ul $450 In stock

Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(80%), Bovine(80%), Horse(90%), Sheep(80%)
Clonality:
Polyclonal
Specificity:
VEGFB Antibody detects endogenous levels of total VEGFB.
RRID:
AB_2835323
Cite Format: Affinity Biosciences Cat# AF7019, RRID:AB_2835323.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Vascular endothelial growth factor B; Vascular endothelial growth factor-related factor; VEGF B; VEGF related factor; VEGF-B; VEGF-related factor; Vegfb; VEGFB_HUMAN; VEGFL; VRF;

Immunogens

Immunogen:
Uniprot:

P49765(VEGFB_HUMAN) >>Visit HPA database.

Gene(ID):
VEGFB(7423)
Expression:
P49765 VEGFB_HUMAN:

Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.

Description:
Several variants of VEGF have been described that arise by alternative mRNA splicing. It has been speculated that VEGF may function as a tumor angiogenesis factor in vivo. Two additional proteins des- ignated VEGF-B and VEGF-C share a significant degree of homology withVEGF.
Sequence:
MSPLLRRLLLAALLQLAPAQAPVSQPDAPGHQRKVVSWIDVYTRATCQPREVVVPLTVELMGTVAKQLVPSCVTVQRCGGCCPDDGLECVPTGQHQVRMQILMIRYPSSQLGEMSLEEHSQCECRPKKKDSAVKPDRAATPHHRPQPRSVPGWDSAPGAPSPADITHPTPAPGPSAHAAPSTTSALTPGPAAAAADAAASSVAKGGA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
90
Pig
80
Bovine
80
Sheep
80
Dog
70
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.

PTMs:

VEGF-B186 is O-glycosylated.

Subcellular Location:

Secreted.
Note: Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.

Subunit Structure:

Homodimer; disulfide-linked. Can also form heterodimer with VEGF.

Family&Domains:

Belongs to the PDGF/VEGF growth factor family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

References

1). Astragaloside IV alleviates liver injury in type 2 diabetes due to promotion of AMPK/mTOR‑mediated autophagy. Molecular Medicine Reports (PubMed: 33846768) [IF=3.4]

Application: WB    Species: Rat    Sample: kidney tissue

Figure 6. AS-IV activates the suppressed autophagy in the liver of T2DM rats. (A) Representative images of Beclin1, LC3 and P62 (immunohistochemical staining; magnification, ×400). Quantitative analysis of (B) Beclin1, (C) LC3 and (D) P62 were normalized to the normal control group. (E) Protein expression levels of Beclin1, LC3 and P62 in the liver tissues were detected via western blotting. Semi-quantitative analysis of (F) Beclin1, (G) LC3 and (H) P62 was normalised to β-actin. Data are presented as the mean ± SD, n=3. *P<0.05, ***P<0.001 vs. control group; #P<0.05, ##P<0.01, ###P<0.001 vs. model group. Con, Control; Mod, Model; AS-IV, Astragaloside IV (80 mg/kg); Met, Metformin (200 mg/kg).
2). Preventive and inhibitive effects of Yiwei Xiaoyu granules on the development and progression of spasmolytic polypeptide-expressing metaplasia lesions. World Journal of Gastrointestinal Oncology (PubMed: 34853647) [IF=3.0]

Application: IHC    Species: Mice    Sample:

Figure 9 The expression of vascular endothelial growth factor-β was restored with Yiwei Xiaoyu granule administration in the spasmolytic polypeptide-expressing metaplasia lesions. A: The expression of vascular endothelial growth factor-β was upregulated with Yiwei Xiaoyu granules administration by immunohistochemistry measurement. B: Statistical differences existed between the control and the model (bP < 0.01). YWXY-H: High dose of Yiwei Xiaoyu Granules; YWXY-L: Low dose of Yiwei Xiaoyu Granules; VEGF: Vascular endothelial growth factor.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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