TIMP2 Antibody - #AF7008

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Product: TIMP2 Antibody
Catalog: AF7008
Description: Rabbit polyclonal antibody to TIMP2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 24kDa; 24kD(Calculated).
Uniprot: P16035
RRID: AB_2835316

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 50ul $250 In stock
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Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(85%), Horse(100%), Sheep(92%), Rabbit(85%), Dog(92%), Chicken(85%)
Clonality:
Polyclonal
Specificity:
TIMP2 Antibody detects endogenous levels of total TIMP2.
RRID:
AB_2835316
Cite Format: Affinity Biosciences Cat# AF7008, RRID:AB_2835316.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CSC 21K; CSC-21K; CSC21K; Metalloproteinase inhibitor 2; Metalloproteinase inhibitor 2 precursor; TIMP 2; TIMP metallopeptidase inhibitor 2; TIMP-2; TIMP2; TIMP2_HUMAN; Tissue Inhibitor of Metalloproteinase 2; Tissue inhibitor of metalloproteinases 2;

Immunogens

Immunogen:
Uniprot:

P16035(TIMP2_HUMAN) >>Visit HPA database.

Gene(ID):
TIMP2(7077)
Description:
TIMP2 Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
Sequence:
MGAAARTLRLALGLLLLATLLRPADACSCSPVHPQQAFCNADVVIRAKAVSEKEVDSGNDIYGNPIKRIQYEIKQIKMFKGPEKDIEFIYTAPSSAVCGVSLDVGGKKEYLIAGKAEGDGKMHITLCDFIVPWDTLSTTQKKSLNHRYQMGCECKITRCPMIPCYISSPDECLWMDWVTEKNINGHQAKFFACIKRSDGSCAWYRGAAPPKQEFLDIEDP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Sheep
92
Dog
92
Bovine
85
Chicken
85
Rabbit
85
Xenopus
77
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P16035 As Substrate

Site PTM Type Enzyme
K53 Ubiquitination
S57 Phosphorylation
Y62 Phosphorylation
K67 Ubiquitination
K74 Ubiquitination
Y90 Phosphorylation
Y165 Phosphorylation

Research Backgrounds

Function:

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.

PTMs:

The activity of TIMP2 is dependent on the presence of disulfide bonds.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts (via the C-terminal) with MMP2 (via the C-terminal PEX domain); the interaction inhibits the MMP2 activity.

Family&Domains:

Belongs to the protease inhibitor I35 (TIMP) family.

References

1). Prenatal Fumonisin Exposure Impairs Bone Development via Disturbances in the OC/Leptin and RANKL/RANK/OPG Systems in Weaned Rat Offspring. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023 (PubMed: 37240089) [IF=5.6]

Application: WB    Species: Rat    Sample:

Figure 4 The effect of exposure of pregnant Wistar rat dams to fumonisins on COMP, VEGF, MMP-8, OPG, RANKL and TIMP-2 protein expression in female (a) and male (b) offspring femora. The protein levels are normalized to the corresponding β-actin levels and expressed as the fold change relative to the amount present in 0 FB control group. 0 FB—the control group; 60 FB—the group of offspring of pregnant rats receiving during pregnancy fumonisins at the dose of 60 FB/kg b.w.; 90 FB—the group of offspring of pregnant rats receiving during pregnancy fumonisins at the dose of 90 FB/kg b.w. Data are mean values ± SD (n = 3). Uncropped original Western blot membranes can be found in Supplementary Figure S1. Statistical significance
2). Effects of lumican expression on the apoptosis of scleral fibroblasts: In vivo and in vitro experiments. Experimental and Therapeutic Medicine, 2021 (PubMed: 33791004) [IF=2.7]

Application: IHC    Species: Rat    Sample: scleral tissue

Figure 2 Expression of lumican, MMP-2, MMP-14 and TIMP-2 in scleral tissue. Expression levels of lumican, MMP-2, MMP-14 and TIMP-2 expression at the (A) mRNA and (B) protein levels, detected by reverse transcription-quantitative PCR and western blotting, respectively. (C) Immunohistochemical analysis of lumican, MMP-2, MMP-14 and TIMP-2 expression. (D) Quantification of results presented in panel C. *P<0.05 vs. control eyes (paired t-tests). TIMP, tissue inhibitor of metalloproteinases.

Application: WB    Species: Rat    Sample: scleral tissue

Figure 5 MMP-2, MMP-14 and TIMP-2 expression in rat scleral fibroblasts following lumican overexpression or knockdown. *P<0.05 vs. the control group (one-way ANOVA and Tukey's post hoc tests). TIMP, tissue inhibitor of metalloproteinases.

Application: IHC    Species: rat    Sample: scleral

Figure 2. |Expression of lumican, MMP‑2, MMP‑14 and TIMP‑2 in scleral tissue. (C) Immunohistochemical analysis of lumican, MMP‑2, MMP‑14 and TIMP‑2 expression.
3). Comparison of MMP‐2 and TIMP‐2 expressions in yak testes at different ages. Reproduction in Domestic Animals, 2023 (PubMed: 36932642) [IF=1.7]

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