Product: AP1S2 Antibody
Catalog: DF16115
Description: Rabbit polyclonal antibody to AP1S2
Application: IHC IF/ICC
Reactivity: Human, Mouse
Mol.Wt.: 19kD(Calculated).
Uniprot: P56377

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
AP1S2 Antibody detects endogenous levels of AP1S2.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Adapter related protein complex 1 sigma 1B subunit; Adapter-related protein complex 1 sigma-1B subunit; Adaptor protein complex AP 1 sigma 1B subunit; Adaptor protein complex AP-1 sigma-1B subunit; Adaptor related protein complex 1 sigma 2 subunit; AP 1 complex subunit sigma 2; AP-1 complex subunit sigma-2; ap1s2; AP1S2_HUMAN; Clathrin adaptor complex AP1 sigma 1B subunit; Clathrin assembly protein complex 1 sigma 1B small chain; Clathrin assembly protein complex 1 sigma-1B small chain; Clathrin associated assembly adaptor protein small 1 like; DC22; Golgi adaptor HA1 AP1 adaptin sigma 1B subunit; Golgi adaptor HA1/AP1 adaptin sigma-1B subunit; mental retardation X linked 59; MGC:1902; MRX59; Sigma 1B subunit of AP 1 clathrin; Sigma 1B subunit of AP-1 clathrin; Sigma adaptin 1B; Sigma-adaptin 1B; Sigma1B adaptin; SIGMA1B; Sigma1B-adaptin;

Immunogens

Immunogen:

A synthesized peptide derived from human AP1S2.

Uniprot:
Gene(ID):
Expression:
P56377 AP1S2_HUMAN:

Widely expressed.

Sequence:
MQFMLLFSRQGKLRLQKWYVPLSDKEKKKITRELVQTVLARKPKMCSFLEWRDLKIVYKRYASLYFCCAIEDQDNELITLEIIHRYVELLDKYFGSVCELDIIFNFEKAYFILDEFLLGGEVQETSKKNVLKAIEQADLLQEEAETPRSVLEEIGLT

Research Backgrounds

Function:

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

Subcellular Location:

Golgi apparatus. Cytoplasmic vesicle membrane>Peripheral membrane protein>Cytoplasmic side. Membrane>Clathrin-coated pit.
Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Subunit Structure:

Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3). Binds to MUC1.

Family&Domains:

Belongs to the adaptor complexes small subunit family.

Research Fields

· Cellular Processes > Transport and catabolism > Lysosome.   (View pathway)

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