Ki67 Antibody - #DF16055
| Product: | Ki67 Antibody |
| Catalog: | DF16055 |
| Description: | Rabbit polyclonal antibody to Ki67 |
| Application: | ELISA(peptide) |
| Reactivity: | Human, Mouse |
| Mol.Wt.: | 351kD(Calculated). |
| Uniprot: | E9PVX6 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Immunogens
A synthesized peptide derived from human Ki67.
- E9PVX6 KI67_MOUSE:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASSAHLVTIKRSGDDGAHFPLSLSSCLFGRSIECDIRIQLPVVSKRHCKIEVKEQEAILYNFSSTNPTQVNGVTIDEPVRLRHGDIITIIDRSFRYEDGNHEDGSKPTEFPGKSLGKEPSRRASRDSFCADPDGEGQDTKASKMTASRRSFVYAKGLSADSPASDGSKNSVSQDSSGHVEQHTGRNIVEPTSGDLFKKSRSTGSSYREPKSSPTQSLSNSNEKESPFEKLYQSMKEELDVKSQKSCRKSEPQPDRAAEESRETQLLVSGRARAKSSGSTPVTAASSPKVGKIWTERWRGGMVPVQTSTETAKMKTPVRHSQQLKDEDSRVTGRRHSVNLDEGESAQAVHKTVTPGKLATRNQAAVEAGDVASPADTPEHSSSKKRSIPAKVEAPSAETQKRLSLTQRLVPGEKKTPKGSFSKPEKLATAAEQTCSGLPGLSSVDISNFGDSINKSEGMPMKRRRVSFGGHLRPELFDENLPPNTPLKRGETPTKRKSLGTHSPAVLKTIIKERPQSPGKQESPGITPPRTNDQRRRSGRTSSGSKFLCETDIPKKAGRKSGNLPAKRASISRSQHGILQMICSKRRSGASEANLIVAKSWADVVKLGVKQTQTKVAKHVPQKQTSKRQRRPSTPKKPTSNLHNQFTTGHANSPCTIVVGRAQIEKVSVPARPYKMLNNLMLNRKVDFSEDLSGLTEMFKTPVKEKQQQMSDTGSVLSNSANLSERQLQVTNSGDIPEPITTEILGEKVLSSTRNAAKQQSDRYSASPTLRRRSIKHENTVQTPKNVHNITDLEKKTPVSETEPLKTASSVSKLRRSRELRHTLVETMNEKTEAVLAENTTARHLRGTFREQKVDQQVQDNENAPQRCKESGELSEGSEKTSARRSSARKQKPTKDLLGSQMVTQTADYAEELLSQGQGTIQNLEESMHMQNTSISEDQGITEKKVNIIVYATKEKHSPKTPGKKAQPLEGPAGLKEHFETPNPKDKPITEDRTRVLCKSPQVTTENITTNTKPQTSTSGKKVDMKEESSALTKRIHMPGESRHNPKILKLECEDIKALKQSENEMLTSTVNGSKRTLEKSKKKAQPLEDLTCFQELFISPVPTNIIKKIPSKSPHTQPVRTPASTKRLSKTGLSKVDVRQEPSTLGKRTKSPGRAPGTPAPVQEENDSTAFMETPKQKLDFAGNSSGSKRRSRTSKNRSQPLEDLDGFQELFQTPAGASDSVTVEESAKISLESSQAEPVKTPASTKRRSKMSLMKVDMKELSILEKQTQSRGRDAGTPAPMQEGNGTTAIMETPKQKLDFTGNSTGHKRRPRTPKIRAQPLEDLDGFQELFQTPAGANDSVTVEESAKMSLESSQAEPVKTPASTKRLSKTDLSKVDVREDPSILGKKTKSPGRAPGTPAPVQEENDCTAYMETPKQKLESIENLTGLRKQSRTPKDITGFQDSFQIPDHANGPLVVVKTKKMFFNSPQPESAITRKSRERQSRASISKIDVKEELLESEEHLQLGEGVDTFQVSTNKVIRSSRKPAKRKLDSTAGMPNSKRMRCSSKDNTPCLEDLNGFQELFQMPGYANDSLTTGISTMLARSPQLGPVRTQINKKSLPKIILRKMDVTEEISGLWKQSLGRVHTTQEQEDNAIKAIMEIPKETLQTAADGTRLTRQPQTPKEKVQPLEDHSVFQELFQTSRYCSDPLIGNKQTRMSLRSPQPGFVRTPRTSKRLAKTSVGNIAVREKISPVSLPQCATGEVVHIPIGPEDDTENKGVKESTPQTLDSSASRTVSKRQQGAHEERPQFSGDLFHPQELFQTPASGKDPVTVDETTKIALQSPQPGHIINPASMKRQSNMSLRKDMREFSILEKQTQSRGRDAGTPAPMQEENGTTAIMETPKQKLDFIGNSTGHKRRPRTPKNRAQPLEDLDGFQELFQTPAGASDPVSVEESAKISLASSQAEPVRTPASTKRRSKTGLSKVDVRQEPSTLGKRMKSLGRAPGTPAPVQEENDSTAFMETPKQKLDFTGNSSGHKRRPQTPKIRAQPLEDLDGFQELFQTPAGANDSVTVEESVKMSLESSQAEPVKTPASTKRLSKTGLSKVDVREDPSILEKKTKSPGTPAPVQEENDCTAFMETPKQKLDFTGNSSGHKRRPRTPKIRAQPLEDLDGFQELFQTPAGASDSVTVEESAKMSLESSQAKPVKTPASTKRLSKTGLSKVDVREDPSTLGKKTKSPGRAPGTPAPVQEENDSTAFMETPKQKLDFAENSSGSKRRSRTSKNRSQPLEDLDGFQELFQTPAGASNPVSVEESAKISLESSQAEPVRTRASTKRLSKTGLNKMDVREGHSPLSKSSCASQKVMQTLTLGEDHGRETKDGKVLLAQKLEPAIYVTRGKRQQRSCKKRSQSPEDLSGVQEVFQTSGHNKDSVTVDNLAKLPSSSPPLEPTDTSVTSRRQARTGLRKVHVKNELSGGIMHPQISGEIVDLPREPEGEGKVIKTRKQSVKRKLDTEVNVPRSKRQRITRAEKTLEDLPGFQELCQAPSLVMDSVIVEKTPKMPDKSPEPVDTTSETQARRRLRRLVVTEEPIPQRKTTRVVRQTRNTQKEPISDNQGMEEFKESSVQKQDPSVSLTGRRNQPRTVKEKTQPLEELTSFQEETAKRISSKSPQPEEKETLAGLKRQLRIQLINDGVKEEPTAQRKQPSRETRNTLKEPVGDSINVEEVKKSTKQKIDPVASVPVSKRPRRVPKEKAQALELAGLKGPIQTLGHTDESASDKGPTQMPCNSLQPEQVDSFQSSPRRPRTRRGKVEADEEPSAVRKTVSTSRQTMRSRKVPEIGNNGTQVSKASIKQTLDTVAKVTGSRRQLRTHKDGVQPLEVLGDSKEITQISDHSEKLAHDTSILKSTQQQKPDSVKPLRTCRRVLRASKEDPKEVLVDTRDHATLQSKSNPLLSPKRKSARDGSIVRTRALRSLAPKQEATDEKPVPEKKRAASSKRHVSPEPVKMKHLKIVSNKLESVEEQVSTVMKTEEMEAKRENPVTPDQNSRYRKKTNVKQPRPKFDASAENVGIKKNEKTMKTASQETELQNPDDGAKKSTSRGQVSGKRTCLRSRGTTEMPQPCEAEEKTSKPAAEILIKPQEEKGVSGESDVRCLRSRKTRVALDSEPKPRVTRGTKKDAKTLKEDEDIVCTKKLRTRS
Research Backgrounds
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (By similarity). Does not contribute to the internal structure of mitotic chromosomes. May play a role in chromatin organization. It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.
Phosphorylated. Hyperphosphorylated in mitosis. Hyperphosphorylated form does not bind DNA.
Chromosome. Nucleus. Nucleus>Nucleolus.
Note: Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:8834799, PubMed:12355204). Associates with satellite DNA in G1 phase (By similarity). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (By similarity). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (By similarity).
Mainly present in proliferating cells (at protein level).
Interacts with KIF15 (By similarity). Interacts (via the FHA domain) with NIFK (By similarity). Interacts with PPP1CC (By similarity). Component of a complex at least composed of ZNF335, HCFC1, CCAR2, EMSY, MKI67, RBBP5, ASH2L and WDR5; the complex is formed as a result of interactions between components of a nuclear receptor-mediated transcription complex and a histone methylation complex (By similarity). Interacts with ZNF335 (By similarity).
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