Product: p62 Dok Antibody
Catalog: AF6478
Description: Rabbit polyclonal antibody to p62 Dok
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 62kDa; 52kD(Calculated).
Uniprot: Q99704
RRID: AB_2835297

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
p62 Dok Antibody detects endogenous levels of total p62 Dok.
RRID:
AB_2835297
Cite Format: Affinity Biosciences Cat# AF6478, RRID:AB_2835297.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Docking protein 1 62kD; Docking protein 1; DOK 1; DOK1; DOK1_HUMAN; Downstream of tyrosine kinase 1; p62(dok); P62DOK; pp62;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q99704 DOK1_HUMAN:

Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells. Expressed in breast cancer.

Description:
Dok1 a docking protein that interacts with receptor tyrosine kinases. It is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase.
Sequence:
MDGAVMEGPLFLQSQRFGTKRWRKTWAVLYPASPHGVARLEFFDHKGSSSGGGRGSSRRLDCKVIRLAECVSVAPVTVETPPEPGATAFRLDTAQRSHLLAADAPSSAAWVQTLCRNAFPKGSWTLAPTDNPPKLSALEMLENSLYSPTWEGSQFWVTVQRTEAAERCGLHGSYVLRVEAERLTLLTVGAQSQILEPLLSWPYTLLRRYGRDKVMFSFEAGRRCPSGPGTFTFQTAQGNDIFQAVETAIHRQKAQGKAGQGHDVLRADSHEGEVAEGKLPSPPGPQELLDSPPALYAEPLDSLRIAPCPSQDSLYSDPLDSTSAQAGEGVQRKKPLYWDLYEHAQQQLLKAKLTDPKEDPIYDEPEGLAPVPPQGLYDLPREPKDAWWCQARVKEEGYELPYNPATDDYAVPPPRSTKPLLAPKPQGPAFPEPGTATGSGIKSHNSALYSQVQKSGASGSWDCGLSRVGTDKTGVKSEGST

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
78
Chicken
60
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99704 As Substrate

Site PTM Type Enzyme
M1 Acetylation
Y30 Phosphorylation
S33 Phosphorylation
R39 Methylation
K46 Ubiquitination
S48 Phosphorylation
T93 Phosphorylation
K121 Ubiquitination
S123 Phosphorylation
T125 Phosphorylation
Y146 Phosphorylation
Y203 Phosphorylation
S217 Phosphorylation
K257 Ubiquitination
S269 Phosphorylation
S281 Phosphorylation
S291 Phosphorylation
Y296 Phosphorylation
C308 S-Nitrosylation
S310 Phosphorylation
S313 Phosphorylation
Y315 Phosphorylation
S316 Phosphorylation
Y337 Phosphorylation
Y341 Phosphorylation
T354 Phosphorylation
Y362 Phosphorylation P00519 (ABL1) , P06213 (INSR) , Q13882 (PTK6)
Y377 Phosphorylation
K394 Ubiquitination
Y398 Phosphorylation P07949 (RET) , P06213 (INSR)
Y402 Phosphorylation
T406 Phosphorylation
Y409 Phosphorylation
T435 Phosphorylation
S439 Phosphorylation O14920 (IKBKB)
S443 Phosphorylation O14920 (IKBKB)
S446 Phosphorylation O14920 (IKBKB)
Y449 Phosphorylation
S450 Phosphorylation O14920 (IKBKB)
S458 Phosphorylation
S460 Phosphorylation
T470 Phosphorylation
T481 Phosphorylation

Research Backgrounds

Function:

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.

PTMs:

Constitutively tyrosine-phosphorylated. Phosphorylated by TEC (By similarity). Phosphorylated by LYN (By similarity). Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway. Phosphorylated on tyrosine residues by SRMS.

Subcellular Location:

Cytoplasm. Nucleus.

Cytoplasm>Perinuclear region.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells. Expressed in breast cancer.

Subunit Structure:

Interacts with ABL1 (By similarity). Interacts with RasGAP and INPP5D/SHIP1. Interacts directly with phosphorylated ITGB3. Interacts with SRMS (via the SH2 and SH3 domains).

Family&Domains:

The PTB domain mediates receptor interaction.

Belongs to the DOK family. Type A subfamily.

Research Fields

· Human Diseases > Infectious diseases: Viral > Measles.

References

1). Advanced oxidation protein products upregulate ABCB1 expression and activity via HDAC2-Foxo3α-mediated signaling in vitro and in vivo. Toxicology and Applied Pharmacology, 2022 (PubMed: 35753429) [IF=3.8]

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