CPI17alpha Antibody | Affinity Biosciences

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Figure 3 Effects of CAS on the expression of phosphorylated CPI-17 in rat coronary arteries.

Product:	CPI17alpha Antibody
Catalog:	AF6473
Description:	Rabbit polyclonal antibody to CPI17alpha
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Sheep, Rabbit
Mol.Wt.:	22kDa; 17kD(Calculated).
Uniprot:	Q96A00
RRID:	AB_2835292

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%)

Clonality:

Polyclonal

Specificity:

CPI17alpha Antibody detects endogenous levels of total CPI17alpha.

RRID:

AB_2835292
Cite Format: Affinity Biosciences Cat# AF6473, RRID:AB_2835292.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

17 kDa PKC-potentiated inhibitory protein of PP1; 17-KDa protein; 17kDa PKC potentiated inhibitory protein of PP1; CPI 17 alpha; CPI 17; CPI 17alpha; CPI-17; CPI17; PKC potentiated inhibitory protein of PP1; PKC potentiated inhibitory protein of PP1, 17-KD; PP14A_HUMAN; PPP1INL; Ppp1r14a; Protein kinase C-potentiated inhibitor of protein phosphatase 1, 17-KD; Protein kinase C-potentiated inhibitor protein of 17 kDa; Protein phosphatase 1; Protein phosphatase 1 regulatory (inhibitor) subunit 14A; Protein phosphatase 1 regulatory subunit 14A; Regulatory subunit 14A;

Immunogens

Immunogen:

Uniprot:

Q96A00(PP14A_HUMAN) >>Visit HPA database.

Gene(ID):

PPP1R14A(94274)

Expression:

Q96A00 PP14A_HUMAN:

Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta.

Description:

PPP1R14A a phosphorylation-dependent inhibitory subunit of protein phosphatase 1 (PP-1A). Phosphorylation by PKC regulates its binding to PP-1A. Acts co-operatively to inhibit myosin light chain phosphatase (MLCP) activity.

Sequence:

Q96A00 PP14A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAQRLGKRVLSKLQSPSRARGPGGSPGGLQKRHARVTVKYDRRELQRRLDVEKWIDGRLEELYRGMEADMPDEINIDELLELESEEERSRKIQGLLKSCGKPVEDFIQELLAKLQGLHRQPGLRQPSPSHDGSLSPLQDRARTAHP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Rabbit

100

Xenopus

Horse

Dog

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96A00 As Substrate

Q96A00

Site	PTM Type	Enzyme	Source
R5	Methylation		Uniprot
S12	Phosphorylation	O43293 (DAPK3)	Uniprot
S16	Phosphorylation		Uniprot
R19	Methylation		Uniprot
R21	Methylation		Uniprot
S26	Phosphorylation		Uniprot
K32	Methylation		Uniprot
T38	Phosphorylation	Q05655 (PRKCD) , P17612 (PRKACA) , O75116 (ROCK2) , Q02156 (PRKCE) , O43293 (DAPK3) , Q15139 (PRKD1) , Q13976 (PRKG1) , P17252 (PRKCA) , Q13418 (ILK) , Q05513 (PRKCZ) , Q13153 (PAK1) , P98161 (PKD1)	Uniprot
K40	Methylation		Uniprot
S128	Phosphorylation		Uniprot
S130	Phosphorylation	Q9UQM7 (CAMK2A)	Uniprot
S134	Phosphorylation		Uniprot
S136	Phosphorylation		Uniprot

Research Backgrounds

Q96A00_PP14A_HUMAN

Function:

Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.

Subcellular Location:

Cytoplasm.

Tissue Specificity:

Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta.

Family&Domains:

Belongs to the PP1 inhibitor family.

Research Fields

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)

References

1). Phosphorylated CPI-17 and MLC2 as Biomarkers of Coronary Artery Spasm-Induced Sudden Cardiac Death. International journal of molecular sciences, 2024 (PubMed: 38474189) [IF=5.6]

Application: WB Species: Rat Sample:

Figure 3 Effects of CAS on the expression of phosphorylated CPI-17 in rat coronary arteries. (A,B) Representative photomicrographs and semi-quantitative data of p-CPI-17 protein levels detected via IHC staining. Lower enlarged images are from upper images. n = 6 per group. Scale bar: 100 µm (upper images) or 50 µm (lower images). (C) Representative immunofluorescent images of p-CPI-17 (red) and DAPI (blue) in the coronary arteries of rats. Scale bar: 50 µm. (D) The mean fluorescence intensity of p-CPI-17 per section was quantified. n = 6 per group. (E,F) Western blot and quantitative analysis of p-CPI-17 protein levels of rat coronary arteries. n = 6 per group. Data presented as mean ± SEM. ** p < 0.01 vs. control group. CPI-17: C kinase-potentiated protein phosphatase 1 inhibitor of 17 kDa; p-CPI-17: phosphorylated C kinase-potentiated protein phosphatase 1 inhibitor of 17 kDa.

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CPI17alpha Antibody - #AF6473