Estrogen Receptor-beta Antibody - #AF6469

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Product: Estrogen Receptor-beta Antibody
Catalog: AF6469
Description: Rabbit polyclonal antibody to Estrogen Receptor-beta
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Horse, Sheep, Dog, Chicken
Mol.Wt.: 59kDa; 59kD(Calculated).
Uniprot: Q92731
RRID: AB_2835288

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Horse(100%), Sheep(89%), Dog(100%), Chicken(86%)
Clonality:
Polyclonal
Specificity:
Estrogen Receptor-beta Antibody detects endogenous levels of total Estrogen Receptor-beta.
RRID:
AB_2835288
Cite Format: Affinity Biosciences Cat# AF6469, RRID:AB_2835288.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ER BETA; ER-beta; Erb; ESR B; ESR BETA; ESR2; ESR2_HUMAN; ESRB; ESTRB; estrogen nuclear receptor beta variant a; estrogen nuclear receptor beta variant b; estrogen receptor 2 (ER beta); Estrogen receptor 2; estrogen receptor beta 4; Estrogen receptor beta; NR3A2; Nuclear receptor subfamily 3 group A member 2;

Immunogens

Immunogen:
Uniprot:

Q92731(ESR2_HUMAN) >>Visit HPA database.

Gene(ID):
ESR2(2100)
Expression:
Q92731 ESR2_HUMAN:

Isoform 1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform 2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform 4 is found in testis. Isoform 5 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform 6 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.

Description:
Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses.
Sequence:
MDIKNSPSSLNSPSSYNCSQSILPLEHGSIYIPSSYVDSHHEYPAMTFYSPAVMNYSIPSNVTNLEGGPGRQTTSPNVLWPTPGHLSPLVVHRQLSHLYAEPQKSPWCEARSLEHTLPVNRETLKRKVSGNRCASPVTGPGSKRDAHFCAVCSDYASGYHYGVWSCEGCKAFFKRSIQGHNDYICPATNQCTIDKNRRKSCQACRLRKCYEVGMVKCGSRRERCGYRLVRRQRSADEQLHCAGKAKRSGGHAPRVRELLLDALSPEQLVLTLLEAEPPHVLISRPSAPFTEASMMMSLTKLADKELVHMISWAKKIPGFVELSLFDQVRLLESCWMEVLMMGLMWRSIDHPGKLIFAPDLVLDRDEGKCVEGILEIFDMLLATTSRFRELKLQHKEYLCVKAMILLNSSMYPLVTATQDADSSRKLAHLLNAVTDALVWVIAKSGISSQQQSMRLANLLMLLSHVRHASNKGMEHLLNMKCKNVVPVYDLLLEMLNAHVLRGCKSSITGSECSPAEDSKSKEGSQNPQSQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Sheep
89
Chicken
86
Pig
78
Bovine
78
Rabbit
67
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92731 As Substrate

Site PTM Type Enzyme
Y36 Phosphorylation P00519 (ABL1)
Y56 Phosphorylation
S75 Phosphorylation
S87 Phosphorylation
S105 Phosphorylation P28482 (MAPK1)
S135 Phosphorylation
S165 Phosphorylation
S176 Phosphorylation
S200 Phosphorylation
S463 Phosphorylation
S469 Phosphorylation
Y488 Phosphorylation

Research Backgrounds

Function:

Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.

PTMs:

Phosphorylation at Ser-87 and Ser-105 recruits NCOA1.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform 2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform 4 is found in testis. Isoform 5 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform 6 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.

Subunit Structure:

Binds DNA as a homodimer. Can form a heterodimer with ESR1. Interacts with NCOA1, NCOA3, NCOA5 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with PELP1 and UBE1C. Isoform 2 preferentially forms a heterodimer with ESR1 rather than ESR2 and inhibits DNA-binding by ESR1. Interacts with AKAP13. Interacts with DNTTIP2. Interacts with isoform 4 of TXNRD1. Interacts with CCDC62 in the presence of estradiol/E2; this interaction seems to enhance the transcription of target genes, including cyclin-D1/CCND1 AP-1 promoter. Interacts with DNAAF4 (By similarity). Interacts with PRMT2. Interacts with CCAR2 (via N-terminus) in a ligand-independent manner. Interacts with RBM39, in the presence of estradiol (E2) (By similarity).

Family&Domains:

Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

Belongs to the nuclear hormone receptor family. NR3 subfamily.

Research Fields

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Prolactin signaling pathway.   (View pathway)

References

1). Low-dose phthalates promote breast cancer stem cell properties via the oncogene ΔNp63α and the Sonic hedgehog pathway. Ecotoxicology and Environmental Safety, 2023 (PubMed: 36753971) [IF=6.8]
2). Genistein accelerates glucose catabolism via activation the GPER-mediated cAMP/PKA-AMPK signaling pathway in broiler chickens. Life Sciences, 2022 (PubMed: 35640778) [IF=6.1]
3). Crocetin Alleviates Ovariectomy-Induced Metabolic Dysfunction through Regulating Estrogen Receptor β. Journal of agricultural and food chemistry, 2021 (PubMed: 34851635) [IF=6.1]
4). Jiawei Shoutai Pill promotes decidualization by regulating the SGK1/ENaC pathway in recurrent spontaneous abortion. Journal of ethnopharmacology, 2024 (PubMed: 37479068) [IF=5.4]

Application: IHC    Species: Human    Sample: decidua tissue

Fig. 3. The expression of SGK1, ENaC-α, ERβ and PR in decidua tissue was detected by immunohistochemistry. Note: vs Control group, ***P < 0.001. Scale bars: a 100 μm, b 50 μm (n = 10).
5). The Role of Estrogen Receptor α in Response to Longitudinal Bone Growth in ob/ob Mice. Frontiers in Endocrinology, 2021 (PubMed: 34925230) [IF=5.2]

Application: WB    Species: Mice    Sample: femoral and spine

Figure 5 The expression level of estrogen receptors (ERs) in femur and spine. (A) Western blotting results of expression level of ERα and ERβ in femoral and spine growth plate (GP), n = 3. (B–E) Semiquantitative analysis of ER expression levels. (F) Quantitative real-time PCR results of ER genes in femoral and vertebral GP, n = 4. (G–J) Immunohistochemistry (IHC) results of expression level of ERα and ERβ in femoral and vertebral GP, n = 6. Scale bar: 20 μm. (K) Comparison of ERα HSCORE between femoral and spine GP (FGP, femoral growth plate; SGP, spine growth plate). (L, M) Results of Spearman’s analysis between ERα HSCORE and femur/spine length. *p < 0.05, ***p < 0.001 and ****p < 0.0001; ns denotes not significant.

Application: IHC    Species: Mice    Sample: femoral and vertebral

Figure 5 The expression level of estrogen receptors (ERs) in femur and spine. (A) Western blotting results of expression level of ERα and ERβ in femoral and spine growth plate (GP), n = 3. (B–E) Semiquantitative analysis of ER expression levels. (F) Quantitative real-time PCR results of ER genes in femoral and vertebral GP, n = 4. (G–J) Immunohistochemistry (IHC) results of expression level of ERα and ERβ in femoral and vertebral GP, n = 6. Scale bar: 20 μm. (K) Comparison of ERα HSCORE between femoral and spine GP (FGP, femoral growth plate; SGP, spine growth plate). (L, M) Results of Spearman’s analysis between ERα HSCORE and femur/spine length. *p < 0.05, ***p < 0.001 and ****p < 0.0001; ns denotes not significant.
6). Ormeloxifene, a selective estrogen receptor modulator, protects against pulmonary hypertension. European Journal of Pharmacology, 2023 (PubMed: 36731722) [IF=5.0]
7). Estrogen receptors and hypoxia inducible factor 1 alpha expression in abdominal wall endometriosis. REPRODUCTIVE BIOMEDICINE ONLINE, 2020 (PubMed: 32444257) [IF=4.0]

Application: IHC    Species: human    Sample: endometrium

FIGURE 1| Expression and localization of oestrogen receptors and hypoxia-inducible factor-1alpha (HIF-1a) proteins in proliferative phase and secretary phase of normal control endometrium analysed by immunohistochemistry. (A–D) ERa; (E–H) ERb; (I–L) G protein-coupled oestrogen receptor (GPER); (M–P) HIF-1a. Micrographs were taken at magnifications of × 200 (left panels) and marked areas in left-hand panels shown at × 400 (right panels) respectively. ER, oestrogen receptor.
8). TLR4 inhibition suppresses growth in oestrogen-induced prolactinoma models. Endocrine-Related Cancer, 2022 (PubMed: 36219868) [IF=3.9]
9). Involvement of estrogen receptor activation in kaempferol-3-O-glucoside's protection against aging-related cognition impairment and microglial inflammation. Experimental cell research, 2023 (PubMed: 37926343) [IF=3.7]
10). Secoisolariciresinol diglucoside regulates estrogen receptor expression to ameliorate OVX-induced osteoporosis. Journal of orthopaedic surgery and research, 2023 (PubMed: 37875947) [IF=2.6]

Application: WB    Species: Rat    Sample:

Fig. 5 SDG promoted the level of estradiol (E2) and the expression of ERα and ERβ of the femur in OVX rats. A-D IHC result and quantitative statistical result of ERα (200 × , scale bar = 50 μm; 400 × , scale bar = 20 μm). E The serum level of E2 was detected by ELISA (n = 6). The expression of ERα and ERβ was determined by IHC and Western Blot. F Representative protein bands of ERα and ERβ. G-H The corresponding quantitative statistical results of ERα and ERβ (n = 3). All data are expressed as the mean ± SD. P## 
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