Product: CDC37 Antibody
Catalog: AF6458
Description: Rabbit polyclonal antibody to CDC37
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 44kDa; 44kD(Calculated).
Uniprot: Q16543
RRID: AB_2835278

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
CDC37 Antibody detects endogenous levels of total CDC37.
RRID:
AB_2835278
Cite Format: Affinity Biosciences Cat# AF6458, RRID:AB_2835278.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CDC 37; Cdc37; CDC37 cell division cycle 37 homolog; CDC37 cell division cycle 37 S cerevisiae homolog; CDC37 cell division cycle 37, S cerevisiae, homolog of; Cdc37 homolog; CDC37 protein; CDC37_HUMAN; CDC37A; cell division cycle 37; Cell division cycle 37 homolog; Hsp90 chaperone protein kinase targeting subunit; Hsp90 chaperone protein kinase targeting subunit p50Cdc37; Hsp90 chaperone protein kinase-targeting subunit; Hsp90 co chaperone Cdc37; Hsp90 co-chaperone Cdc37; p50; p50Cdc37; S cerevisiae hypothetical protein CDC37;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
The protein encoded by this gene is highly similar to Cdc 37, a cell division cycle control protein of Sacchromyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction.
Sequence:
MVDYSVWDHIEVSDDEDETHPNIDTASLFRWRHQARVERMEQFQKEKEELDRGCRECKRKVAECQRKLKELEVAEGGKAELERLQAEAQQLRKEERSWEQKLEEMRKKEKSMPWNVDTLSKDGFSKSMVNTKPEKTEEDSEEVREQKHKTFVEKYEKQIKHFGMLRRWDDSQKYLSDNVHLVCEETANYLVIWCIDLEVEEKCALMEQVAHQTIVMQFILELAKSLKVDPRACFRQFFTKIKTADRQYMEGFNDELEAFKERVRGRAKLRIEKAMKEYEEEERKKRLGPGGLDPVEVYESLPEELQKCFDVKDVQMLQDAISKMDPTDAKYHMQRCIDSGLWVPNSKASEAKEGEEAGPGDPLLEAVPKTGDEKDVSV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16543 As Substrate

Site PTM Type Enzyme
M1 Acetylation
V2 Acetylation
Y4 Phosphorylation
S5 Phosphorylation
S13 Phosphorylation P19784 (CSNK2A2) , P68400 (CSNK2A1)
K45 Acetylation
K67 Ubiquitination
K69 Acetylation
K69 Ubiquitination
K78 Acetylation
K93 Ubiquitination
S97 Phosphorylation
K101 Ubiquitination
K110 Ubiquitination
S111 Phosphorylation
T118 Phosphorylation
S120 Phosphorylation
K121 Ubiquitination
K126 Ubiquitination
S127 Phosphorylation
T131 Phosphorylation
K132 Sumoylation
K135 Ubiquitination
T136 Phosphorylation
S140 Phosphorylation
K154 Acetylation
K157 Acetylation
K240 Acetylation
R246 Methylation
K260 Ubiquitination
Y278 Phosphorylation
Y298 Phosphorylation P07947 (YES1)
S300 Phosphorylation
K307 Ubiquitination
C308 S-Nitrosylation
K312 Ubiquitination
S322 Phosphorylation
K323 Ubiquitination
K330 Acetylation
K330 Ubiquitination
C336 S-Nitrosylation
S339 Phosphorylation
S346 Phosphorylation
K347 Ubiquitination
K352 Ubiquitination
K369 Ubiquitination
T370 Phosphorylation
K374 Ubiquitination
S377 Phosphorylation

Research Backgrounds

Function:

Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity. Inhibits HSP90AA1 ATPase activity.

PTMs:

Constitutively sumoylated by UBE2I.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones STIP1/HOP, CDC37, PPP5C, PTGES3/p23, TSC1 and client protein TSC2. Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones CDC37, PPP5C, TSC1 and client protein TSC2, CDK4, AKT, RAF1 and NR3C1; this complex does not contain co-chaperones STIP1/HOP and PTGES3/p23. Forms a complex with Hsp90/HSP90AB1 and CDK6. Interacts with HSP90AA1. Interacts with AR, CDK4, CDK6 and EIF2AK1. Interacts with RB1 (By similarity). Interacts with KSR1. Interacts with FLCN, FNIP1 and FNIP2.

Family&Domains:

Belongs to the CDC37 family.

Research Fields

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

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