Smad1 Antibody - #AF6451

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Product: Smad1 Antibody
Catalog: AF6451
Description: Rabbit polyclonal antibody to Smad1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 60kDa; 52kD(Calculated).
Uniprot: Q15797
RRID: AB_2835273

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Smad1 Antibody detects endogenous levels of total Smad1.
RRID:
AB_2835273
Cite Format: Affinity Biosciences Cat# AF6451, RRID:AB_2835273.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BSP-1; BSP1; HsMAD1; JV4-1; JV41; MAD homolog 1; MAD mothers against decapentaplegic homolog 1; Mad related protein 1; Mad-related protein 1; MADH1; MADR1; Mothers against decapentaplegic homolog 1; Mothers against DPP homolog 1; SMA- AND MAD-RELATED PROTEIN 1; SMAD 1; SMAD family member 1; SMAD mothers against DPP homolog 1; Smad1; SMAD1_HUMAN; TGF beta signaling protein 1; Transforming growth factor-beta-signaling protein 1;

Immunogens

Immunogen:
Uniprot:

Q15797(SMAD1_HUMAN) >>Visit HPA database.

Gene(ID):
SMAD1(4086)
Expression:
Q15797 SMAD1_HUMAN:

Ubiquitous. Highest expression seen in the heart and skeletal muscle.

Description:
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways.
Sequence:
MNVTSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSCPGQPSNCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYKRVESPVLPPVLVPRHSEYNPQHSLLAQFRNLGQNEPHMPLNATFPDSFQQPNSHPFPHSPNSSYPNSPGSSSSTYPHSPTSSDPGSPFQMPADTPPPAYLPPEDPMTQDGSQPMDTNMMAPPLPSEINRGDVQAVAYEEPKHWCSIVYYELNNRVGEAFHASSTSVLVDGFTDPSNNKNRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECLSDSSIFVQSRNCNYHHGFHPTTVCKIPSGCSLKIFNNQEFAQLLAQSVNHGFETVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNPISSVS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15797 As Substrate

Site PTM Type Enzyme
Ubiquitination
S11 Phosphorylation
K21 Ubiquitination
K32 Ubiquitination
S78 Phosphorylation
K81 Ubiquitination
Y88 Phosphorylation
K116 Sumoylation
K116 Ubiquitination
K118 Sumoylation
K118 Ubiquitination
S132 Phosphorylation
S144 Phosphorylation
Y146 Phosphorylation
S187 Phosphorylation P50750 (CDK9) , P49336 (CDK8) , P28482 (MAPK1)
S195 Phosphorylation P49336 (CDK8) , P50750 (CDK9) , P28482 (MAPK1)
T202 Phosphorylation P49841 (GSK3B)
S206 Phosphorylation P50750 (CDK9) , P28482 (MAPK1) , P49336 (CDK8) , P50613 (CDK7)
S210 Phosphorylation
S214 Phosphorylation P28482 (MAPK1) , P50750 (CDK9) , P49336 (CDK8)
S239 Phosphorylation Q13315 (ATM)
K269 Ubiquitination
S315 Phosphorylation
T322 Phosphorylation Q8N4C8 (MINK1) , Q9UKE5 (TNIK)
K418 Ubiquitination
S456 Phosphorylation
S462 Phosphorylation O00238 (BMPR1B)
S463 Phosphorylation Q05655 (PRKCD) , P36894 (BMPR1A) , O00238 (BMPR1B)
S465 Phosphorylation Q05655 (PRKCD) , O00238 (BMPR1B) , P36894 (BMPR1A)

Research Backgrounds

Function:

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression.

PTMs:

Phosphorylation of the C-terminal SVS motif by BMP type 1 receptor kinase activates SMAD1 by promoting dissociation from the receptor and trimerization with SMAD4.

Ubiquitinated by SMAD-specific E3 ubiquitin ligase SMURF1, leading to its degradation. Monoubiquitinated, leading to prevent DNA-binding. Deubiquitination by USP15 alleviates inhibition and promotes activation of TGF-beta target genes. Dephosphorylation, probably by PPM1A, induces its export from the nucleus to the cytoplasm (By similarity).

Subcellular Location:

Cytoplasm. Nucleus.
Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4 (PubMed:15647271). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15647271). Exported from the nucleus to the cytoplasm when dephosphorylated (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Highest expression seen in the heart and skeletal muscle.

Subunit Structure:

Found in a complex with SMAD4 and YY1. Interacts with HGS, NANOG and ZCCHC12 (By similarity). Upon C-terminus phosphorylation: forms trimers with another SMAD1 and the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit, CREB-binding protein (CBP), p300, SMURF1, SMURF2, USP15 and HOXC8. Associates with ZNF423 or ZNF521 in response to BMP2 leading to activate transcription of BMP target genes. Interacts with SKOR1. Interacts (via MH2 domain) with LEMD3. Binding to LEMD3 results in at least a partial reduction of receptor-mediated phosphorylation. Forms a ternary complex with PSMB4 and OAZ1 before PSMB4 is incorporated into the 20S proteasome. Found in a macromolecular complex with FAM83G. Interacts (via MH2 domain) with FAM83G (via MH2 domain); in a SMAD4-independent manner. Interacts with ZC3H3 (By similarity). Interacts with TMEM119 (By similarity). Interacts (via MH1 and MH2 domains) with ZNF8 (By similarity). Interacts with RANBP3L; the interaction increases when SMAD1 is not phosphorylated and mediates SMAD1 nuclear export.

Family&Domains:

The MH2 domain mediates phosphorylation-dependent trimerization through L3 loop binding of phosphoserines in the adjacent subunit.

Belongs to the dwarfin/SMAD family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

References

1). Maternal supplementation with mulberry-leaf flavonoids improves the development of skeletal muscle in the offspring of chickens. Animal nutrition (Zhongguo xu mu shou yi xue hui), 2024 (PubMed: 39035983) [IF=6.3]
2). Inokosterone activates the BMP2 to promote the osteogenic differentiation of bone marrow mesenchymal stem cells and improve bone loss in ovariectomized rats. Biochemical and biophysical research communications, 2023 (PubMed: 37839103) [IF=3.1]

Application: WB    Species: Rat    Sample: BMSCs

Fig. 1. Inokosterone (IS) promoted cell viability and osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs). (A) Morphological observation of primary cultured BMSCs at 1, 3, 5, and 7 days ( × 100, Scale bar = 100 μm). Then, BMSCs were cultured in the osteogenic induction and differentiation medium with/without IS (50, 100, and 200 mg/L) for 72 h. (B) IS (50, 100, and 200 mg/L) promoted cell viability of BMSCs, which was measured by the cell counting kit-8 analysis (n = 5). (C) IS advanced the levels of collagen type I (collagen I), alkaline phosphatase (ALP), and osteocalcin (OCN) of cultured supernatant, which was detected by the enzyme-linked immunosorbent assay (n = 6). (D) Alizarin red S (ARS) staining showed the area of ARS staining of BMSC with IS treatment was increased (n = 3). (E) They are characteristic photos of alizarin red S staining ( × 400, Scale bar = 300 μm). The expression levels of (F) bone morphogenetic protein-2 (BMP2), (G) smad1, (H) runt-related transcription factor 2 (RUNX2), (I) collagen I, (J) ALP in BMSCs were measured by Western blot (n = 3). IS promoted their expression. (K) The characteristic photos of Western blot. The experiment was repeated thrice, and all data were expressed with mean ± standard deviation. *P < 0.05, **P < 0.01, compared to the control (CN) group.

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