F2RL2 Antibody - #DF15686

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Product: F2RL2 Antibody
Catalog: DF15686
Description: Rabbit polyclonal antibody to F2RL2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 43kD(Calculated).
Uniprot: O00254

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
F2RL2 Antibody detects endogenous levels of F2RL2.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

coagulation factor II (thrombin) receptor-like 2; Coagulation factor II receptor-like 2; F2RL2; PAR 3; PAR-3; PAR3; PAR3_HUMAN; protease activated receptor 3; Proteinase-activated receptor 3; thrombin receptor like 2; Thrombin receptor-like 2;

Immunogens

Immunogen:

A synthesized peptide derived from human F2RL2.

Uniprot:

O00254(PAR3_HUMAN) >>Visit HPA database.

Gene(ID):
F2RL2(2151)
Expression:
O00254 PAR3_HUMAN:

Highest expression in the megakaryocytes of the bone marrow, lower in mature megakaryocytes, in platelets and in a variety of other tissues such as heart and gut.

Sequence:
MKALIFAAAGLLLLLPTFCQSGMENDTNNLAKPTLPIKTFRGAPPNSFEEFPFSALEGWTGATITVKIKCPEESASHLHVKNATMGYLTSSLSTKLIPAIYLLVFVVGVPANAVTLWMLFFRTRSICTTVFYTNLAIADFLFCVTLPFKIAYHLNGNNWVFGEVLCRATTVIFYGNMYCSILLLACISINRYLAIVHPFTYRGLPKHTYALVTCGLVWATVFLYMLPFFILKQEYYLVQPDITTCHDVHNTCESSSPFQLYYFISLAFFGFLIPFVLIIYCYAAIIRTLNAYDHRWLWYVKASLLILVIFTICFAPSNIILIIHHANYYYNNTDGLYFIYLIALCLGSLNSCLDPFLYFLMSKTRNHSTAYLTK

Research Backgrounds

Function:

Receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis.

PTMs:

A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highest expression in the megakaryocytes of the bone marrow, lower in mature megakaryocytes, in platelets and in a variety of other tissues such as heart and gut.

Subunit Structure:

Interacts with INSC/inscuteable and probably GPSM2.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Immune system > Complement and coagulation cascades.   (View pathway)

References

1). Weighted Correlation Network Analysis of Cancer Stem Cell-Related Prognostic Biomarkers in Esophageal Squamous Cell Carcinoma. Technology in Cancer Research & Treatment, 2022 (PubMed: 35899307) [IF=2.8]

Application: IF/ICC    Species: Human    Sample: esophageal squamous cell carcinoma (ESCC)

Figure 3. Representative immunofluorescence of CILP (red), CST1 (red), DPP4 (red), F2RL2 (green), PITX2 (green), RIOX (red), ZFHX4 (green) in esophageal squamous cell carcinoma (ESCC) samples and normal tissues of 112 ESCC patients from Zhongshan Hospital, Fudan University.

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