BRK1 Antibody - #DF15680

(1)  
Product: BRK1 Antibody
Catalog: DF15680
Description: Rabbit polyclonal antibody to BRK1
Application: IF/ICC
Reactivity: Human, Mouse
Mol.Wt.: 9kD(Calculated).
Uniprot: Q8WUW1

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
BRK1 Antibody detects endogenous levels of BRK1.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BRICK1, SCAR/WAVE actin nucleating complex subunit; BRICK1, SCAR/WAVE actin nucleating complex subunit, homolog; BRK1; Brk1-like; C3orf10 chromosome 3 open reading frame 10; C3ORF10 GENE; Chromosome 3 open reading frame 10; Haematopoietic stem/progenitor cell protein 300; hHBrk1; HSPC300; MDS027; Probable protein BRICK1; Protein BRICK1;

Immunogens

Immunogen:

A synthesized peptide derived from human BRK1.

Uniprot:

Q8WUW1(BRK1_HUMAN) >>Visit HPA database.

Gene(ID):
BRK1(55845)
Sequence:
MAGQEDPVQREIHQDWANREYIEIITSSIKKIADFLNSFDMSCRSRLATLNEKLTALERRIEYIEARVTKGETLT

Research Backgrounds

Function:

Involved in regulation of actin and microtubule organization. Part of a WAVE complex that activates the Arp2/3 complex. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (By similarity).

Subcellular Location:

Cytoplasm>Cytoskeleton.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homotrimer when in free form. Directly interacts with WASF2. Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterodimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1.

Family&Domains:

Belongs to the BRK1 family.

Research Fields

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

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