ACC1 Antibody | Affinity Biosciences

Product:	ACC1 Antibody
Catalog:	AF6421
Description:	Rabbit polyclonal antibody to ACC1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	265kDa; 266kD(Calculated).
Uniprot:	Q13085
RRID:	AB_2835251

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(85%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(92%), Dog(100%), Chicken(80%)

Clonality:

Polyclonal

Specificity:

ACC1 Antibody detects endogenous levels of total ACC1.

RRID:

AB_2835251
Cite Format: Affinity Biosciences Cat# AF6421, RRID:AB_2835251.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ACAC;ACACA;ACACA_HUMAN;ACC alpha;ACC 1;ACC-alpha;ACC1;ACCA;Acetyl CoA carboxylase 1;Acetyl CoA carboxylase alpha;Acetyl Coenzyme A carboxylase alpha;Biotin carboxylase;COA1;HACC275 antibody;

Immunogens

Immunogen:

Uniprot:

Q13085(ACACA_HUMAN) >>Visit HPA database.

Gene(ID):

ACACA(31)

Expression:

Q13085 ACACA_HUMAN:

Expressed in brain, placenta, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.

Description:

ACC1 a subunit of acetyl-CoA carboxylase (ACC), a multifunctional enzyme system. Catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis.

Sequence:

Q13085 ACACA_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDEPSPLAQPLELNQHSRFIIGSVSEDNSEDEISNLVKLDLLEEKEGSLSPASVGSDTLSDLGISSLQDGLALHIRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWQENDFSKRILNVPQELYEKGYVKDVDDGLQAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATPAVFEHMEQCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLYRIKDIRMMYGVSPWGDSPIDFEDSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQMNRIDTGWLDRLIAEKVQAERPDTMLGVVCGALHVADVSLRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGVKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVMRSPSAGKLIQYIVEDGGHVFAGQCYAEIEVMKMVMTLTAVESGCIHYVKRPGAALDPGCVLAKMQLDNPSKVQQAELHTGSLPRIQSTALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSKVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQRYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGRDPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMTHVASVSDVLLDNSFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFSDSPPQSPTFPEAGHTSLYDEDKVPRDEPIHILNVAIKTDCDIEDDRLAAMFREFTQQNKATLVDHGIRRLTFLVAQKDFRKQVNYEVDRRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIKLWESMSTQAFLPSPPLPSDMLTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMTFKSPEYPEGRDIIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPRIYVSANSGARIGLAEEIRHMFHVAWVDPEDPYKGYRYLYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGIGPENLRGSGMIAGESSLAYNEIITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHCTVCDDFEGVFTVLHWLSYMPKSVHSSVPLLNSKDPIDRIIEFVPTKTPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFSEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDGLRECCQPVLVYIPPQAELRGGSWVVIDSSINPRHMEMYADRESRGSVLEPEGTVEIKFRRKDLVKTMRRVDPVYIHLAERLGTPELSTAERKELENKLKEREEFLIPIYHQVAVQFADLHDTPGRMQEKGVISDILDWKTSRTFFYWRLRRLLLEDLVKKKIHNANPELTDGQIQAMLRRWFVEVEGTVKAYVWDNNKDLAEWLEKQLTEEDGVHSVIEENIKCISRDYVLKQIRSLVQANPEVAMDSIIHMTQHISPTQRAEVIRILSTMDSPST

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Dog

100

Horse

Rabbit

Zebrafish

Chicken

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13085 As Substrate

Q13085

Site	PTM Type	Enzyme	Source
M1	Acetylation		Uniprot
S5	Phosphorylation		Uniprot
S23	Phosphorylation		Uniprot
S25	Phosphorylation		Uniprot
S29	Phosphorylation		Uniprot
S34	Phosphorylation		Uniprot
K45	Ubiquitination		Uniprot
S48	Phosphorylation		Uniprot
S50	Phosphorylation		Uniprot
S53	Phosphorylation		Uniprot
S56	Phosphorylation		Uniprot
T58	Phosphorylation		Uniprot
S60	Phosphorylation		Uniprot
S77	Phosphorylation	P17612 (PRKACA)	Uniprot
S78	Phosphorylation	P54646 (PRKAA2)	Uniprot
S80	Phosphorylation	P54646 (PRKAA2) , Q13131 (PRKAA1)	Uniprot
K86	Ubiquitination		Uniprot
S96	Phosphorylation		Uniprot
S104	Phosphorylation		Uniprot
K116	Ubiquitination		Uniprot
K120	Ubiquitination		Uniprot
K132	Ubiquitination		Uniprot
S234	Phosphorylation		Uniprot
K276	Ubiquitination		Uniprot
K288	Ubiquitination		Uniprot
Y290	Phosphorylation		Uniprot
K292	Ubiquitination		Uniprot
K311	Ubiquitination		Uniprot
S344	Phosphorylation		Uniprot
K386	Ubiquitination		Uniprot
K410	Ubiquitination		Uniprot
Y417	Phosphorylation		Uniprot
Y434	Phosphorylation		Uniprot
S488	Phosphorylation		Uniprot
K520	Ubiquitination		Uniprot
K579	Ubiquitination		Uniprot
T610	Phosphorylation		Uniprot
Y676	Phosphorylation		Uniprot
Y734	Phosphorylation		Uniprot
K747	Ubiquitination		Uniprot
S756	Phosphorylation		Uniprot
K817	Ubiquitination		Uniprot
K825	Ubiquitination		Uniprot
S835	Phosphorylation		Uniprot
S841	Phosphorylation		Uniprot
Y855	Phosphorylation		Uniprot
Y867	Phosphorylation		Uniprot
K879	Ubiquitination		Uniprot
S893	Phosphorylation		Uniprot
S907	Phosphorylation		Uniprot
K916	Ubiquitination		Uniprot
S977	Phosphorylation		Uniprot
Y994	Phosphorylation		Uniprot
T1042	Phosphorylation		Uniprot
T1073	Phosphorylation		Uniprot
K1076	Ubiquitination		Uniprot
T1196	Phosphorylation		Uniprot
S1201	Phosphorylation	P17612 (PRKACA)	Uniprot
Y1209	Phosphorylation		Uniprot
S1216	Phosphorylation		Uniprot
S1218	Phosphorylation		Uniprot
T1227	Phosphorylation		Uniprot
S1257	Phosphorylation		Uniprot
S1259	Phosphorylation		Uniprot
S1263	Phosphorylation		Uniprot
T1265	Phosphorylation		Uniprot
S1273	Phosphorylation		Uniprot
K1316	Ubiquitination		Uniprot
K1334	Acetylation		Uniprot
K1334	Ubiquitination		Uniprot
K1338	Ubiquitination		Uniprot
Y1342	Phosphorylation		Uniprot
K1354	Ubiquitination		Uniprot
Y1370	Phosphorylation		Uniprot
K1435	Ubiquitination		Uniprot
S1438	Phosphorylation		Uniprot
Y1441	Phosphorylation		Uniprot
Y1499	Phosphorylation		Uniprot
K1524	Ubiquitination		Uniprot
K1580	Acetylation		Uniprot
K1586	Ubiquitination		Uniprot
S1720	Phosphorylation		Uniprot
Y1745	Phosphorylation		Uniprot
K1759	Acetylation		Uniprot
K1759	Ubiquitination		Uniprot
K1781	Ubiquitination		Uniprot
K1788	Acetylation		Uniprot
K1788	Ubiquitination		Uniprot
S1844	Phosphorylation		Uniprot
K1856	Acetylation		Uniprot
K1856	Ubiquitination		Uniprot
S1908	Phosphorylation		Uniprot
K1916	Ubiquitination		Uniprot
K1929	Ubiquitination		Uniprot
K2031	Acetylation		Uniprot
K2031	Ubiquitination		Uniprot
R2047	Methylation		Uniprot
S2099	Phosphorylation		Uniprot
Y2108	Phosphorylation		Uniprot
K2127	Acetylation		Uniprot
K2127	Ubiquitination		Uniprot
K2135	Ubiquitination		Uniprot
Y2144	Phosphorylation		Uniprot
T2153	Phosphorylation		Uniprot
K2209	Ubiquitination		Uniprot
K2231	Ubiquitination		Uniprot
K2268	Ubiquitination		Uniprot
K2293	Ubiquitination		Uniprot
K2302	Ubiquitination		Uniprot
S2318	Phosphorylation		Uniprot
S2343	Phosphorylation		Uniprot
S2345	Phosphorylation		Uniprot

Research Backgrounds

Q13085_ACACA_HUMAN

Function:

Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis. This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA.

PTMs:

Phosphorylation on Ser-1263 is required for interaction with BRCA1.

Phosphorylation at Ser-80 by AMPK inactivates enzyme activity.

The biotin cofactor is covalently attached to the central biotinyl-binding domain and is required for the catalytic activity.

Subcellular Location:

Cytoplasm>Cytosol.

Tissue Specificity:

Expressed in brain, placenta, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.

Subunit Structure:

Monomer, homodimer, and homotetramer. Can form filamentous polymers. Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis. Interacts with MID1IP1; interaction with MID1IP1 promotes oligomerization and increases its activity.

Family&Domains:

Consists of an N-terminal biotin carboxylation/carboxylase (BC) domain that catalyzes the ATP-dependent transient carboxylation of the biotin covalently attached to the central biotinyl-binding/biotin carboxyl carrier (BCC) domain (Probable). The C-terminal carboxyl transferase (CT) domain catalyzes the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA to produce malonyl-CoA (Probable).

Research Fields

· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)

· Metabolism > Lipid metabolism > Fatty acid biosynthesis.

· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.

· Metabolism > Carbohydrate metabolism > Propanoate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Fatty acid metabolism.

· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

References

1). Yogurt-derived Lactobacillus plantarum Q16 alleviated high-fat diet-induced non-alcoholic fatty liver disease in mice. Food Science and Human Wellness, 2022 [IF=7.0]

Application: WB Species: Mouse Sample:

Fig. 5. Effects ofL. plantarum Q16 on key proteins involved in hepatic lipid metabolism in HFD-fed obese mice. Data are presented as mean ± SD (n = 6). Different lowercase alphabet letters were significantly different at the level of P < 0.05.

2). Puerariae Lobatae radix flavonoids and puerarin alleviate alcoholic liver injury in zebrafish by regulating alcohol and lipid metabolism. BIOMEDICINE & PHARMACOTHERAPY, 2021 (PubMed: 33341668) [IF=6.9]

Application: WB Species: zebrafish Sample: zebrafish larvae

Fig. 8. The effect of PLF and puerarin on protein expression. (A) Protein expression level of AMPKα, p- AMPKα (Thr172) and ACC1. (B) Expression levels of pAMPKα/AMPKα. (C) Quantitation of western blotting analysis of ACC1. **P < 0.005 in comparison with the control group. ##P < 0.005 in comparison with the 2 % EtOH group.

3). Amelioration of nonalcoholic fatty liver disease by swertiamarin in fructose-fed mice. Phytomedicine, 2019 (PubMed: 31005808) [IF=6.7]

Application: WB Species: mouse Sample: liver

Figure.7. |Effect of swertiamarin (25, 50 and 100 mg/kg) and silibinin on (A) ACC1 expression by western blotting analysis. Immunohistochemical stainings of (B) SREBP-1 and (C) FAS in liver tissue of mice. Scale bar represents 30 μm. Representative analysis from six animals in each group.Significance is represented as ##P ≤ 0.01 and ###P ≤ 0.001 compared to the normal control group,*P ≤ 0.05 and **P ≤ 0.01 compared to the fructose group.

4). α-Hydroxyisocaproic Acid Decreases Protein Synthesis but Attenuates TNFα/IFNγ Co-Exposure-Induced Protein Degradation and Myotube Atrophy via Suppression of iNOS and IL-6 in Murine C2C12 Myotube. Nutrients, 2021 (PubMed: 34371902) [IF=5.9]

Application: WB Species: Mice Sample:

Figure 2 The effects of HICA on the intracellular signaling pathways. A typical image for a capillary immunoassay is shown (A). The phosphorylation levels of (B) p70S6K and 4E-BP1; (C) AMPK, ACC, and ULK1; (D) ERK1/2; (E) p38MAPK; and (F) eEF2 are shown. The phosphorylation is normalized to the total protein expression. The β-tubulin content in the lysate was measured as a loading control (G). The time course of these experiments is shown in the upper region. DM: differentiation medium, DMEM: Dulbecco’s modified Eagle’s medium, and w/o AA: without amino acids. Data are displayed as the means ± SD, and n = 4 for each group in all bar graphs. * p < 0.05 and ** p < 0.01 vs. the vehicle-treated group.

5). Amphibian pore-forming protein βγ-CAT drives extracellular nutrient scavenging under cell nutrient deficiency. iScience, 2022 (PubMed: 37128610) [IF=5.8]

6). Propionate Ameliorates Alcohol-Induced Liver Injury in Mice via the Gut–Liver Axis: Focus on the Improvement of Intestinal Permeability. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2022 (PubMed: 35549256) [IF=5.7]

7). D-Mannose Regulates Hepatocyte Lipid Metabolism via PI3K/Akt/mTOR Signaling Pathway and Ameliorates Hepatic Steatosis in Alcoholic Liver Disease. Frontiers in immunology, 2022 (PubMed: 35464439) [IF=5.7]

Application: WB Species: Mouse Sample:

Figure 4. Mannose inhibits ethanol-induced hepatocyte lipogenesis. (A-C) Isolated PMHs were obtained from mice fed the control diet (Pair) or ethanol diet (EtOH) supplemented with or without 3% (w/v) mannose (Man). The protein (A) and mRNA (B) levels of SREBP1c, ACC1 and FASN were evaluated (n = 6). (C) Representative images of SREBP1c staining on the liver sections (n = 3). Scale bars = 100 μm. (D, E) PMHs from WT mice were stimulated by 200 mM ethanol (EtOH) with/without 5 mM mannose (Man) for 24 h, PMHs with cell culture medium as control (Ctrl). The protein (D) and mRNA (E) levels of lipogenic enzyme genes SREBP1c, ACC1, and FASN were evaluated (n = 3). Data are expressed as the means ± SEM of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns, not significant, unpaired two-tailed t-test.

8). Ferulic acid ameliorates atherosclerotic injury by modulating gut microbiota and lipid metabolism. Frontiers in Pharmacology, 2021 (PubMed: 33841148) [IF=5.6]

Application: WB Species: Mice Sample: liver tissue

FIGURE 5 FA regulated the expression of lipid metabolites-related Proteins in HFD-fed ApoE−/− mice (A), (B) Protein expression of AMPKα, P-AMPKα, SREBP1, ACC1 in liver (n = 3), (C) inmmunofluorescence staining of ACC1, SREBP1 in Liver sections (200 ×). *p < 0.05; as compared to the control group. # p < 0.05, ## p < 0.01; as compared to the model group.

Application: IF/ICC Species: Mice Sample: liver tissue

9). Selegiline ameliorated dyslipidemia and hepatic steatosis in high-fat diet mice. International Immunopharmacology, 2023 (PubMed: 36822098) [IF=4.8]

10). Fanlian Huazhuo Formula alleviates high-fat diet-induced non-alcoholic fatty liver disease by modulating autophagy and lipid synthesis signaling pathway. World journal of gastroenterology, 2024 (PubMed: 39193572) [IF=4.3]

Application: WB Species: Human Sample: HepG2 cells

Figure 5. Fanlian Huazhuo Formula group regulated lipid synthesis signaling pathway in vitro. A: Protein expression levels of AMPKα, SREBP-1C, ACC1, FASN and β-actin in HepG2 cells induced by free fatty acid (n = 3); B: Relative mRNA expression levels of AMPKα, SREBP-1C, ACC1 and FASN in HepG2 cells tested by real-time quantitative polymerase chain reaction (n = 3). Data are presented as mean ± SE. aP < 0.05 vs NC group, cP < 0.05 vs MOD group. NC: Negative control group; MOD: Model group; FLHZF: Fanlian Huazhuo Formula group.

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ACC1 Antibody - #AF6421