ZBTB32 Antibody | Affinity Biosciences

Product:	ZBTB32 Antibody
Catalog:	DF15527
Description:	Rabbit polyclonal antibody to ZBTB32
Application:	ELISA(peptide)
Reactivity:	Human, Mouse
Mol.Wt.:	53kD(Calculated).
Uniprot:	Q9Y2Y4

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Secondary Antibodies
Blocking Peptides

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

ZBTB32 Antibody detects endogenous levels of ZBTB32.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FANCC interacting protein; FANCC-interacting protein; Fanconi anemia zinc finger protein; FAXF; FAZF; OTTHUMP00000045790; repressor of GATA; Rog; Testis zinc finger protein; ZBT32_HUMAN; Zbtb32; ZBTB32 protein; Zinc finger and BTB domain containing 32; Zinc finger and BTB domain-containing protein 32; Zinc finger protein 538; ZNF538;

Immunogens

Immunogen:

A synthesized peptide derived from human ZBTB32.

Uniprot:

Q9Y2Y4(ZBT32_HUMAN) >>Visit HPA database.

Gene(ID):

ZBTB32(27033)

Expression:

Q9Y2Y4 ZBT32_HUMAN:

Predominantly expressed in testis. Some isoforms are ubiquitously expressed.

Sequence:

Q9Y2Y4 ZBT32_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSLPPIRLPSPYGSDRLVQLAARLRPALCDTLITVGSQEFPAHSLVLAGVSQQLGRRGQWALGEGISPSTFAQLLNFVYGESVELQPGELRPLQEAARALGVQSLEEACWRARGDRAKKPDPGLKKHQEEPEKPSRNPERELGDPGEKQKPEQVSRTGGREQEMLHKHSPPRGRPEMAGATQEAQQEQTRSKEKRLQAPVGQRGADGKHGVLTWLRENPGGSEESLRKLPGPLPPAGSLQTSVTPRPSWAEAPWLVGGQPALWSILLMPPRYGIPFYHSTPTTGAWQEVWREQRIPLSLNAPKGLWSQNQLASSSPTPGSLPQGPAQLSPGEMEESDQGHTGALATCAGHEDKAGCPPRPHPPPAPPARSRPYACSVCGKRFSLKHQMETHYRVHTGEKPFSCSLCPQRSRDFSAMTKHLRTHGAAPYRCSLCGAGCPSLASMQAHMRGHSPSQLPPGWTIRSTFLYSSSRPSRPSTSPCCPSSSTT

Research Backgrounds

Q9Y2Y4_ZBT32_HUMAN

Function:

DNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor. Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T-cells (By similarity).

Subcellular Location:

Nucleus.
Note: Located in nuclear speckles.

Tissue Specificity:

Predominantly expressed in testis. Some isoforms are ubiquitously expressed.

Subunit Structure:

Homodimer (via PTB domain). Interacts with the N-terminal of FANCC. Interacts with ZBTB16. Interacts with GATA3 (By similarity).

Family&Domains:

The C-terminal zinc finger domain functions as a transcriptional transactivator.

The BTB (POZ) domain possesses repressor activity.

Belongs to the krueppel C2H2-type zinc-finger protein family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

ZBTB32 Antibody - #DF15527