MARCO Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from Rat brain, using MARCO Antibody.

Product:	MARCO Antibody
Catalog:	DF15505
Description:	Rabbit polyclonal antibody to MARCO
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	53kD(Calculated).
Uniprot:	Q9UEW3

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

MARCO Antibody detects endogenous levels of MARCO.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AI323439; Ly112; Macrophage receptor MARCO; Macrophage receptor with collagenous structure; Marco; MARCO_HUMAN; SCARA2; Scavenger receptor class A member 2;

Immunogens

Immunogen:

A synthesized peptide derived from human MARCO.

Uniprot:

Q9UEW3(MARCO_HUMAN) >>Visit HPA database.

Gene(ID):

MARCO(8685)

Expression:

Q9UEW3 MARCO_HUMAN:

Expressed in alveolar macrophages (at protein level). Detected in macrophages from various tissues including thymus, kidney, Kupffer cells of liver, and spleen (PubMed:9468508).

Sequence:

Q9UEW3 MARCO_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRNKKILKEDELLSETQQAAFHQIAMEPFEINVPKPKRRNGVNFSLAVVVIYLILLTAGAGLLVVQVLNLQARLRVLEMYFLNDTLAAEDSPSFSLLQSAHPGEHLAQGASRLQVLQAQLTWVRVSHEHLLQRVDNFTQNPGMFRIKGEQGAPGLQGHKGAMGMPGAPGPPGPPAEKGAKGAMGRDGATGPSGPQGPPGVKGEAGLQGPQGAPGKQGATGTPGPQGEKGSKGDGGLIGPKGETGTKGEKGDLGLPGSKGDRGMKGDAGVMGPPGAQGSKGDFGRPGPPGLAGFPGAKGDQGQPGLQGVPGPPGAVGHPGAKGEPGSAGSPGRAGLPGSPGSPGATGLKGSKGDTGLQGQQGRKGESGVPGPAGVKGEQGSPGLAGPKGAPGQAGQKGDQGVKGSSGEQGVKGEKGERGENSVSVRIVGSSNRGRAEVYYSGTWGTICDDEWQNSDAIVFCRMLGYSKGRALYKVGAGTGQIWLDNVQCRGTESTLWSCTKNSWGHHDCSHEEDAGVECSV

Research Backgrounds

Q9UEW3_MARCO_HUMAN

Function:

Pattern recognition receptor (PRR) which binds Gram-positive and Gram-negative bacteria. Also plays a role in binding of unopsonized particles by alveolar macrophages (By similarity). Binds to the secretoglobin SCGB3A2.

PTMs:

N-glycosylated.

Subcellular Location:

Cell membrane>Single-pass type II membrane protein.

Tissue Specificity:

Expressed in alveolar macrophages (at protein level). Detected in macrophages from various tissues including thymus, kidney, Kupffer cells of liver, and spleen.

Subunit Structure:

Homotrimer; disulfide-linked. Trimers may assemble in larger oligomers thus resulting in the creation of a large surface capable of interacting with very large ligands.

Research Fields

· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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MARCO Antibody - #DF15505