PEMT Antibody - #DF15389

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Product: PEMT Antibody
Catalog: DF15389
Description: Rabbit polyclonal antibody to PEMT
Application: IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 22kD(Calculated).
Uniprot: Q9UBM1

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
PEMT Antibody detects endogenous levels of PEMT.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

PEAMT; PEMPT; PEMT; PEMT_HUMAN; PEMT2; phosphatidylethanolamine N methyltransferase; Phosphatidylethanolamine N-methyltransferase; PNMT;

Immunogens

Immunogen:

A synthesized peptide derived from human PEMT.

Uniprot:

Q9UBM1(PEMT_HUMAN) >>Visit HPA database.

Gene(ID):
PEMT(10400)
Sequence:
MTRLLGYVDPLDPSFVAAVITITFNPLYWNVVARWEHKTRKLSRAFGSPYLACYSLSVTILLLNFLRSHCFTQAMLSQPRMESLDTPAAYSLGLALLGLGVVLVLSSFFALGFAGTFLGDYFGILKEARVTVFPFNILDNPMYWGSTANYLGWAIMHASPTGLLLTVLVALTYIVALLYEEPFTAEIYRQKASGSHKRS

Research Backgrounds

Function:

Catalyzes the three sequential steps of the methylation pathway of phosphatidylcholine biosynthesis, the SAM-dependent methylation of phosphatidylethanolamine (PE) to phosphatidylmonomethylethanolamine (PMME), PMME to phosphatidyldimethylethanolamine (PDME), and PDME to phosphatidylcholine (PC).

PTMs:

Isoform 2 is N-glycosylated with high-mannose oligosaccharides.

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein. Mitochondrion membrane>Multi-pass membrane protein.
Note: Found in endoplasmic reticulum where most PEMT activity is generated and in mitochondria.

Endoplasmic reticulum membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the class VI-like SAM-binding methyltransferase superfamily. PEMT/PEM2 methyltransferase family.

Research Fields

· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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