HSL Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of HSL expression in CalyculinA treated HeLa whole cell lysates,The lane on the left was treated with the antigen-specific peptide.

AF6402 staining Hela cells by IF/ICC.

Product:	HSL Antibody
Catalog:	AF6402
Description:	Rabbit polyclonal antibody to HSL
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	81kDa,83kDa; 117kD(Calculated).
Uniprot:	Q05469
RRID:	AB_2835233

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(90%)

Clonality:

Polyclonal

Specificity:

HSL Antibody detects endogenous levels of total HSL.

RRID:

AB_2835233
Cite Format: Affinity Biosciences Cat# AF6402, RRID:AB_2835233.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Hormone sensitive lipase; Hormone sensitive lipase testicular isoform; Hormone-sensitive lipase; HSL; LHS; Lipase hormone sensitive; LIPE; LIPS_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q05469(LIPS_HUMAN) >>Visit HPA database.

Gene(ID):

LIPE(3991)

Description:

hormone sensitive lipase is a lipolytic enzyme of the 'GDXG' family. Plays a rate limiting step in triglyceride lipolysis. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.

Sequence:

Q05469 LIPS_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEPGSKSVSRSDWQPEPHQRPITPLEPGPEKTPIAQPESKTLQGSNTQQKPASNQRPLTQQETPAQHDAESQKEPRAQQKSASQEEFLAPQKPAPQQSPYIQRVLLTQQEAASQQGPGLGKESITQQEPALRQRHVAQPGPGPGEPPPAQQEAESTPAAQAKPGAKREPSAPTESTSQETPEQSDKQTTPVQGAKSKQGSLTELGFLTKLQELSIQRSALEWKALSEWVTDSESESDVGSSSDTDSPATMGGMVAQGVKLGFKGKSGYKVMSGYSGTSPHEKTSARNHRHYQDTASRLIHNMDLRTMTQSLVTLAEDNIAFFSSQGPGETAQRLSGVFAGVREQALGLEPALGRLLGVAHLFDLDPETPANGYRSLVHTARCCLAHLLHKSRYVASNRRSIFFRTSHNLAELEAYLAALTQLRALVYYAQRLLVTNRPGVLFFEGDEGLTADFLREYVTLHKGCFYGRCLGFQFTPAIRPFLQTISIGLVSFGEHYKRNETGLSVAASSLFTSGRFAIDPELRGAEFERITQNLDVHFWKAFWNITEMEVLSSLANMASATVRVSRLLSLPPEAFEMPLTADPTLTVTISPPLAHTGPGPVLVRLISYDLREGQDSEELSSLIKSNGQRSLELWPRPQQAPRSRSLIVHFHGGGFVAQTSRSHEPYLKSWAQELGAPIISIDYSLAPEAPFPRALEECFFAYCWAIKHCALLGSTGERICLAGDSAGGNLCFTVALRAAAYGVRVPDGIMAAYPATMLQPAASPSRLLSLMDPLLPLSVLSKCVSAYAGAKTEDHSNSDQKALGMMGLVRRDTALLLRDFRLGASSWLNSFLELSGRKSQKMSEPIAEPMRRSVSEAALAQPQGPLGTDSLKNLTLRDLSLRGNSETSSDTPEMSLSAETLSPSTPSDVNFLLPPEDAGEEAEAKNELSPMDRGLGVRAAFPEGFHPRRSSQGATQMPLYSSPIVKNPFMSPLLAPDSMLKSLPPVHIVACALDPMLDDSVMLARRLRNLGQPVTLRVVEDLPHGFLTLAALCRETRQAAELCVERIRLVLTPPAGAGPSGETGAAGVDGGCGGRH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q05469 As Substrate

Q05469

Site	PTM Type	Enzyme	Source
T188	Phosphorylation		Uniprot
S196	Phosphorylation		Uniprot
S552	Phosphorylation		Uniprot
S565	Phosphorylation		Uniprot
K624	Ubiquitination		Uniprot
S630	Phosphorylation		Uniprot
S645	Phosphorylation		Uniprot
S649	Phosphorylation		Uniprot
S650	Phosphorylation		Uniprot
S660	Phosphorylation		Uniprot
Y741	Phosphorylation		Uniprot
T813	Phosphorylation		Uniprot
S825	Phosphorylation		Uniprot
S835	Phosphorylation		Uniprot
S853	Phosphorylation	P17612 (PRKACA) , Q13976 (PRKG1)	Uniprot
S855	Phosphorylation	Q14012 (CAMK1) , P17612 (PRKACA) , Q13131 (PRKAA1) , Q9UQM7 (CAMK2A)	Uniprot
K872	Ubiquitination		Uniprot
S950	Phosphorylation	P17612 (PRKACA)	Uniprot
S951	Phosphorylation	P17612 (PRKACA)	Uniprot
T955	Phosphorylation		Uniprot
T1015	Phosphorylation		Uniprot

Research Backgrounds

Q05469_LIPS_HUMAN

Function:

In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.

PTMs:

Phosphorylation by AMPK may block translocation to lipid droplets.

Subcellular Location:

Cell membrane. Membrane>Caveola. Cytoplasm>Cytosol.
Note: Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation.

Subunit Structure:

Interacts with CAVIN1 in the adipocyte cytoplasm. Interacts with PLIN5 (By similarity).

Family&Domains:

Belongs to the 'GDXG' lipolytic enzyme family.

Research Fields

· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

References

1). Icariside II suppresses the tumorigenesis and development of ovarian cancer by regulating miR‐144‐3p/IGF2R axis. DRUG DEVELOPMENT RESEARCH (PubMed: 35808943) [IF=3.8]

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

HSL Antibody - #AF6402