USF2 Antibody - #AF0291
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| Product: | USF2 Antibody |
| Catalog: | AF0291 |
| Description: | Rabbit polyclonal antibody to USF2 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 40kDa; 37kD(Calculated). |
| Uniprot: | Q15853 |
| RRID: | AB_2833457 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF0291, RRID:AB_2833457.
Fold/Unfold
bHLHb12; Class B basic helix-loop-helix protein 12; FIP; FOS interacting protein; FOS-interacting protein; Major late transcription factor 2; Upstream stimulatory factor 2; Upstream transcription factor 2; USF 2; Usf2; USF2_HUMAN;
Immunogens
- Q15853 USF2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDMLDPGLDPAASATAAAAASHDKGPEAEEGVELQEGGDGPGAEEQTAVAITSVQQAAFGDHNIQYQFRTETNGGQVTYRVVQVTDGQLDGQGDTAGAVSVVSTAAFAGGQQAVTQVGVDGAAQRPGPAAASVPPGPAAPFPLAVIQNPFSNGGSPAAEAVSGEARFAYFPASSVGDTTAVSVQTTDQSLQAGGQFYVMMTPQDVLQTGTQRTIAPRTHPYSPKIDGTRTPRDERRRAQHNEVERRRRDKINNWIVQLSKIIPDCNADNSKTGASKGGILSKACDYIRELRQTNQRMQETFKEAERLQMDNELLRQQIEELKNENALLRAQLQQHNLEMVGEGTRQ
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q15853 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S155 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| Y221 | Phosphorylation | Uniprot | |
| S222 | Phosphorylation | Uniprot | |
| K224 | Ubiquitination | Uniprot | |
| T228 | Phosphorylation | Uniprot | |
| T230 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| K250 | Ubiquitination | Uniprot | |
| K271 | Ubiquitination | Uniprot | |
| T272 | Phosphorylation | Uniprot | |
| S281 | Phosphorylation | Uniprot | |
| T293 | Phosphorylation | Uniprot | |
| K302 | Ubiquitination | Uniprot | |
| K322 | Ubiquitination | Uniprot |
Research Backgrounds
Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.
Nucleus.
Ubiquitous.
Interacts with MAF (By similarity). Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer (USF1/USF2). In vivo, the USF1/USF2A heterodimer represents over 66% of the usf binding activity whereas the USF1 and USF2A homodimers represent less than 10%. The USF1/USF2B heterodimer accounted for almost 15% in some cell.
References
Application: IHC Species: human Sample: CRC tissues
Application: WB Species: human Sample: HT29 cells
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