UBE1L Antibody | Affinity Biosciences

WB
IHC
Cites

1/3

Western blot analysis of extracts from Hepg2, using UBE1L Antibody.

AF0288 at 1/100 staining human kidney tissue sections by IHC-P.

Figure 8 CSFV infection antagonizes the activation of HSP90AA1 on JAK/STAT pathway.

Product:	UBE1L Antibody
Catalog:	AF0288
Description:	Rabbit polyclonal antibody to UBE1L
Application:	WB IHC IF/ICC
Reactivity:	Human
Prediction:	Pig, Rabbit
Mol.Wt.:	112kDa; 112kD(Calculated).
Uniprot:	P41226
RRID:	AB_2833456

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(85%), Rabbit(85%)

Clonality:

Polyclonal

Specificity:

UBE1L Antibody detects endogenous levels of total UBE1L.

RRID:

AB_2833456
Cite Format: Affinity Biosciences Cat# AF0288, RRID:AB_2833456.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

D8; MGC12713; UBA1, ubiquitin activating enzyme E1 homolog B; UBA1B; UBA7; UBA7, ubiquitin activating enzyme E1; UBA7_HUMAN; Ube 1L; UBE 2; UBE1L; UBE2; Ubiquitin activating enzyme 2; Ubiquitin activating enzyme E1 homolog; Ubiquitin Activating Enzyme E1 Like; Ubiquitin activating enzyme E1 related protein; Ubiquitin-activating enzyme 7; Ubiquitin-activating enzyme E1 homolog; ubiquitin-activating enzyme-2; ubiquitin-like modifier activating enzyme 7; Ubiquitin-like modifier-activating enzyme 7;

Immunogens

Immunogen:

Uniprot:

P41226(UBA7_HUMAN) >>Visit HPA database.

Gene(ID):

UBA7(7318)

Expression:

P41226 UBA7_HUMAN:

Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.

Description:

UBE1L Activates ubiquitin by first adenylating with ATP its C- terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin- E1 thioester and free AMP. Belongs to the ubiquitin-activating E1 family.

Sequence:

P41226 UBA7_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDALDASKLLDEELYSRQLYVLGSPAMQRIQGARVLVSGLQGLGAEVAKNLVLMGVGSLTLHDPHPTCWSDLAAQFLLSEQDLERSRAEASQELLAQLNRAVQVVVHTGDITEDLLLDFQVVVLTAAKLEEQLKVGTLCHKHGVCFLAADTRGLVGQLFCDFGEDFTVQDPTEAEPLTAAIQHISQGSPGILTLRKGANTHYFRDGDLVTFSGIEGMVELNDCDPRSIHVREDGSLEIGDTTTFSRYLRGGAITEVKRPKTVRHKSLDTALLQPHVVAQSSQEVHHAHCLHQAFCALHKFQHLHGRPPQPWDPVDAETVVGLARDLEPLKRTEEEPLEEPLDEALVRTVALSSAGVLSPMVAMLGAVAAQEVLKAISRKFMPLDQWLYFDALDCLPEDGELLPSPEDCALRGSRYDGQIAVFGAGFQEKLRRQHYLLVGAGAIGCELLKVFALVGLGAGNSGGLTVVDMDHIERSNLSRQFLFRSQDVGRPKAEVAAAAARGLNPDLQVIPLTYPLDPTTEHIYGDNFFSRVDGVAAALDSFQARRYVAARCTHYLKPLLEAGTSGTWGSATVFMPHVTEAYRAPASAAASEDAPYPVCTVRYFPSTAEHTLQWARHEFEELFRLSAETINHHQQAHTSLADMDEPQTLTLLKPVLGVLRVRPQNWQDCVAWALGHWKLCFHYGIKQLLRHFPPNKVLEDGTPFWSGPKQCPQPLEFDTNQDTHLLYVLAAANLYAQMHGLPGSQDWTALRELLKLLPQPDPQQMAPIFASNLELASASAEFGPEQQKELNKALEVWSVGPPLKPLMFEKDDDSNFHVDFVVAAASLRCQNYGIPPVNRAQSKRIVGQIIPAIATTTAAVAGLLGLELYKVVSGPRPRSAFRHSYLHLAENYLIRYMPFAPAIQTFHHLKWTSWDRLKVPAGQPERTLESLLAHLQEQHGLRVRILLHGSALLYAAGWSPEKQAQHLPLRVTELVQQLTGQAPAPGQRVLVLELSCEGDDEDTAFPPLHYEL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

Pig

Horse

Dog

Bovine

Chicken

Sheep

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P41226 As Substrate

P41226

Site	PTM Type	Source
K8	Ubiquitination	Uniprot
Y15	Phosphorylation	Uniprot
S24	Phosphorylation	Uniprot
K134	Ubiquitination	Uniprot
K257	Ubiquitination	Uniprot
S266	Phosphorylation	Uniprot
K330	Ubiquitination	Uniprot
K429	Ubiquitination	Uniprot
S485	Phosphorylation	Uniprot
K492	Ubiquitination	Uniprot
Y514	Phosphorylation	Uniprot
Y524	Phosphorylation	Uniprot
Y555	Phosphorylation	Uniprot
K696	Ubiquitination	Uniprot
S879	Phosphorylation	Uniprot
S884	Phosphorylation	Uniprot
Y892	Phosphorylation	Uniprot
Y896	Phosphorylation	Uniprot

Research Backgrounds

P41226_UBA7_HUMAN

Function:

Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Catalyzes the ISGylation of influenza A virus NS1 protein.

PTMs:

ISGylated.

Tissue Specificity:

Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.

Subunit Structure:

Monomer (By similarity). Binds and is involved in the conjugation of G1P2/ISG15.

Family&Domains:

Belongs to the ubiquitin-activating E1 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

References

1). Heme oxygenase-1 prevents heart against myocardial infarction by attenuating ischemic injury-induced cardiomyocytes senescence. EBioMedicine, 2019 (PubMed: 30527623) [IF=11.1]

2). HSP90AA1 interacts with CSFV NS5A protein and regulates CSFV replication via the JAK/STAT and NF-κB signaling pathway. Frontiers in Immunology, 2022 (PubMed: 36405689) [IF=7.3]

Application: WB Species: pig Sample: PK-15 cell

Figure 8 CSFV infection antagonizes the activation of HSP90AA1 on JAK/STAT pathway. (A, B) Western blot for JAK1, STAT1, p-JAK1, p-STAT1, OAS2, ISG-15 and HSP90AA1 expression in HSP90AA1-overexpression or knock-downed PK-15 and 3D4/2 cells. Cells were infected with CSFV (MOI=1) after transfection p3×Flag-HSP90AA1 or siHSP90AA1. The cells were not infected with CSFV as a control. Cells were harvested at 24hpi and 48hpi served to Western blot. The relative levels of proteins were estimated by histograms representing density reading of the gel bands with Image J, and the ratios were calculated relative to tubulin control. (*p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 calculated using two-way ANOVA, ns, not significant).

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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UBE1L Antibody - #AF0288