AP1AR Antibody | Affinity Biosciences

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Western blot analysis of extracts from Hela cells(heat-shock treatment), using AP1AR Antibody.

DF15169 at 1/100 staining mouse muscle tissue by IHC-P.

Product:	AP1AR Antibody
Catalog:	DF15169
Description:	Rabbit polyclonal antibody to AP1AR
Application:	WB IHC
Reactivity:	Human
Mol.Wt.:	35kD; 34kD(Calculated).
Uniprot:	Q63HQ0

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

AP1AR Antibody detects endogenous levels of AP1AR.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

2c18; Adapter-related protein complex 1-associated regulatory protein; AP-1 complex-associated regulatory protein; AP1AR; AP1AR_HUMAN; C4orf16; Chromosome 4 open reading frame 16; Gamma-1-adaptin brefeldin A resistance protein; Gamma-BAR; Gamma1-adaptin brefeldin A resistance protein, gamma-BAR; GBAR; PRO0971;

Immunogens

Immunogen:

A synthesized peptide derived from human AP1AR.

Uniprot:

Q63HQ0(AP1AR_HUMAN) >>Visit HPA database.

Gene(ID):

AP1AR(55435)

Sequence:

Q63HQ0 AP1AR_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGNCCWTQCFGLLRKEAGRLQRVGGGGGSKYFRTCSRGEHLTIEFENLVESDEGESPGSSHRPLTEEEIVDLRERHYDSIAEKQKDLDKKIQKELALQEEKLRLEEEALYAAQREAARAAKQRKLLEQERQRIVQQYHPSNNGEYQSSGPEDDFESCLRNMKSQYEVFRSSRLSSDATVLTPNTESSCDLMTKTKSTSGNDDSTSLDLEWEDEEGMNRMLPMRERSKTEEDILRAALKYSNKKTGSNPTSASDDSNGLEWENDFVSAEMDDNGNSEYSGFVNPVLELSDSGIRHSDTDQQTR

Research Backgrounds

Q63HQ0_AP1AR_HUMAN

Function:

Necessary for adaptor protein complex 1 (AP-1)-dependent transport between the trans-Golgi network and endosomes. Regulates the membrane association of AP1G1/gamma1-adaptin, one of the subunits of the AP-1 adaptor complex. The direct interaction with AP1G1/gamma1-adaptin attenuates the release of the AP-1 complex from membranes. Regulates endosomal membrane traffic via association with AP-1 and KIF5B thus linking kinesin-based plus-end-directed microtubular transport to AP-1-dependent membrane traffic. May act as effector of AP-1 in calcium-induced endo-lysosome secretion. Inhibits Arp2/3 complex function; negatively regulates cell spreading, size and motility via intracellular sequestration of the Arp2/3 complex.

PTMs:

Palmitoylated.

Subcellular Location:

Golgi apparatus>trans-Golgi network. Late endosome. Early endosome.
Note: Localizes to the juxta-nuclear Golgi region and to tubular structures throughout the cytoplasm, which are highly mobile and cycle between the juxta-nuclear area and the cell periphery.

Subunit Structure:

Interacts (via coiled-coil domain) with AP1G1 (via GAE domain). Interacts with KIF5B. Associates with the Arp2/3 complex.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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AP1AR Antibody - #DF15169