Tubulin gamma Antibody | Affinity Biosciences

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Western blot analysis on mouse brain cell lysates using Tubulin gamma Antibody.

AF0285 at 1/100 staining human testis tissue sections by IHC-P.

Product:	Tubulin gamma Antibody
Catalog:	AF0285
Description:	Rabbit polyclonal antibody to Tubulin gamma
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Zebrafish
Mol.Wt.:	51kDa; 51kD(Calculated).
Uniprot:	P23258
RRID:	AB_2833453

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Zebrafish(88%)

Clonality:

Polyclonal

Specificity:

Tubulin gamma Antibody detects endogenous levels of total Tubulin gamma.

RRID:

AB_2833453
Cite Format: Affinity Biosciences Cat# AF0285, RRID:AB_2833453.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Gamma 1 tubulin; Gamma 2 tubulin; Gamma Tubulin 1; Gamma Tubulin 2; Gamma tubulin complex component 1; Gamma-2-tubulin; GCP 1; GCP1; MGC131994; TBG2_HUMAN; TUBG; TUBG1; TUBG2; TUBGCP1; Tubulin gamma 1 chain; Tubulin gamma 2 chain; Tubulin gamma complex-associated protein 1; Tubulin gamma-2 chain; tubulin, gamma 1; tubulin, gamma 2; tubulin, gamma polypeptide; Xgam;

Immunogens

Immunogen:

A synthesized peptide derived from human Tubulin gamma, corresponding to a region within N-terminal amino acids.

Uniprot:

P23258(TBG1_HUMAN) >>Visit HPA database.

Gene(ID):

TUBG1(7283)

Description:

TUBG2 Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation (By similarity).

Sequence:

P23258 TBG1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPREIITLQLGQCGNQIGFEFWKQLCAEHGISPEGIVEEFATEGTDRKDVFFYQADDEHYIPRAVLLDLEPRVIHSILNSPYAKLYNPENIYLSEHGGGAGNNWASGFSQGEKIHEDIFDIIDREADGSDSLEGFVLCHSIAGGTGSGLGSYLLERLNDRYPKKLVQTYSVFPNQDEMSDVVVQPYNSLLTLKRLTQNADCVVVLDNTALNRIATDRLHIQNPSFSQINQLVSTIMSASTTTLRYPGYMNNDLIGLIASLIPTPRLHFLMTGYTPLTTDQSVASVRKTTVLDVMRRLLQPKNVMVSTGRDRQTNHCYIAILNIIQGEVDPTQVHKSLQRIRERKLANFIPWGPASIQVALSRKSPYLPSAHRVSGLMMANHTSISSLFERTCRQYDKLRKREAFLEQFRKEDMFKDNFDEMDTSREIVQQLIDEYHAATRPDYISWGTQEQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Zebrafish

Pig

Horse

Bovine

Sheep

Xenopus

Rabbit

Dog

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P23258 As Substrate

P23258

Site	PTM Type	Enzyme	Source
S32	Phosphorylation		Uniprot
K48	Ubiquitination		Uniprot
S80	Phosphorylation	P24941 (CDK2)	Uniprot
K84	Ubiquitination		Uniprot
K113	Ubiquitination		Uniprot
S129	Phosphorylation		Uniprot
S131	Phosphorylation	Q8TDC3-2 (BRSK1)	Uniprot
K164	Ubiquitination		Uniprot
K193	Ubiquitination		Uniprot
S284	Phosphorylation		Uniprot
K287	Ubiquitination		Uniprot
R295	Methylation		Uniprot
K301	Ubiquitination		Uniprot
S306	Phosphorylation		Uniprot
T307	Phosphorylation		Uniprot
K335	Ubiquitination		Uniprot
S336	Phosphorylation		Uniprot
K344	Ubiquitination		Uniprot
K363	Ubiquitination		Uniprot
S364	Phosphorylation		Uniprot
S385	Phosphorylation	Q8TDC3 (BRSK1)	Uniprot
K397	Acetylation		Uniprot
K400	Acetylation		Uniprot
K400	Ubiquitination		Uniprot
K410	Ubiquitination		Uniprot
K415	Ubiquitination		Uniprot
T423	Phosphorylation		Uniprot
S424	Phosphorylation		Uniprot

Research Backgrounds

P23258_TBG1_HUMAN

Function:

Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation.

PTMs:

Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.

Subunit Structure:

Interacts with TUBGCP2 and TUBGCP3. Interacts with B9D2 (By similarity). Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of TUBG1 and CDK5RAP2. Interacts with PIFO. Interacts with SAS6 and NUP62 at the centrosome.

Family&Domains:

Belongs to the tubulin family.

Research Fields

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

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Tubulin gamma Antibody - #AF0285