NME8 Antibody | Affinity Biosciences

Product:	NME8 Antibody
Catalog:	DF14843
Description:	Rabbit polyclonal antibody to NME8
Application:	ELISA(peptide)
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	67kD(Calculated).
Uniprot:	Q8N427

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Blocking Peptides

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

NME8 Antibody detects endogenous levels of NME8.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CILD6; NM23 H8; NME8; Sperm specific thioredoxin 2; Spermatid specific thioredoxin 2; Sptrx 2; SPTRX2; Thioredoxin domain containing protein 3;

Immunogens

Immunogen:

A synthesized peptide derived from human NME8.

Uniprot:

Q8N427(TXND3_HUMAN) >>Visit HPA database.

Gene(ID):

NME8(51314)

Expression:

Q8N427 TXND3_HUMAN:

Testis-specific. Expressed only in primary spermatocytes and round spermatids.

Sequence:

Q8N427 TXND3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MASKKREVQLQTVINNQSLWDEMLQNKGLTVIDVYQAWCGPCRAMQPLFRKLKNELNEDEILHFAVAEADNIVTLQPFRDKCEPVFLFSVNGKIIEKIQGANAPLVNKKVINLIDEERKIAAGEMARPQYPEIPLVDSDSEVSEESPCESVQELYSIAIIKPDAVISKKVLEIKRKITKAGFIIEAEHKTVLTEEQVVNFYSRIADQCDFEEFVSFMTSGLSYILVVSQGSKHNPPSEETEPQTDTEPNERSEDQPEVEAQVTPGMMKNKQDSLQEYLERQHLAQLCDIEEDAANVAKFMDAFFPDFKKMKSMKLEKTLALLRPNLFHERKDDVLRIIKDEDFKILEQRQVVLSEKEAQALCKEYENEDYFNKLIENMTSGPSLALVLLRDNGLQYWKQLLGPRTVEEAIEYFPESLCAQFAMDSLPVNQLYGSDSLETAEREIQHFFPLQSTLGLIKPHATSEQREQILKIVKEAGFDLTQVKKMFLTPEQIEKIYPKVTGKDFYKDLLEMLSVGPSMVMILTKWNAVAEWRRLMGPTDPEEAKLLSPDSIRAQFGISKLKNIVHGASNAYEAKEVVNRLFEDPEEN

Research Backgrounds

Q8N427_TXND3_HUMAN

Function:

Probably required during the final stages of sperm tail maturation in the testis and/or epididymis, where extensive disulfide bonding of fibrous sheath (FS) proteins occurs. May be involved in the reduction of disulfide bonds within the sperm FS components. In vitro, it has neither NDP kinase nor reducing activity on disulfide bonds.

Subcellular Location:

Cytoplasm.

Tissue Specificity:

Testis-specific. Expressed only in primary spermatocytes and round spermatids.

Subunit Structure:

Monomer.

Family&Domains:

Contains 3 inactive NDK domains that each lack the active His residue, suggesting that they have no NDP kinase activity.

In the C-terminal section; belongs to the NDK family.

Restrictive clause

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For Research Use Only.
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NME8 Antibody - #DF14843