TOP2B Antibody | Affinity Biosciences

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AF0283 at 1/100 staining human brain tissue sections by IHC-P.

Product:	TOP2B Antibody
Catalog:	AF0283
Description:	Rabbit polyclonal antibody to TOP2B
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Bovine, Sheep, Dog, Chicken
Mol.Wt.:	183kDa; 183kD(Calculated).
Uniprot:	Q02880
RRID:	AB_2833451

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Bovine(93%), Sheep(100%), Dog(100%), Chicken(80%)

Clonality:

Polyclonal

Specificity:

TOP2B Antibody detects endogenous levels of total TOP2B.

RRID:

AB_2833451
Cite Format: Affinity Biosciences Cat# AF0283, RRID:AB_2833451.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Antigen MLAA 44; beta isozyme; DNA topoisomerase 2 beta; DNA topoisomerase 2-beta; DNA topoisomerase II 180 kD; DNA topoisomerase II; DNA topoisomerase II beta; DNA topoisomerase II beta isozyme; Top 2; TOP 2B; TOP IIB; Top2 beta; TOP2B; TOP2B_HUMAN; Top2beta; TOPIIB; Topo II beta; Topoisomerase (DNA) II beta 180kDa; Topoisomerase (DNA) II beta; Topoisomerase IIb; U937 associated antigen;

Immunogens

Immunogen:

Uniprot:

Q02880(TOP2B_HUMAN) >>Visit HPA database.

Gene(ID):

TOP2B(7155)

Description:

TOP2B Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Indirectly ivolved in vitamin D- coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR- mediated transrepression of the CYP27B1 gene. Belongs to the type II topoisomerase family.

Sequence:

Q02880 TOP2B_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAKSGGCGAGAGVGGGNGALTWVTLFDQNNAAKKEESETANKNDSSKKLSVERVYQKKTQLEHILLRPDTYIGSVEPLTQFMWVYDEDVGMNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCRGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVKPFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEEDETQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDAKGREVNDLKRKSPSDLWKEDLAAFVEELDKVESQEREDVLAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIIPEITAMKADASKKLLKKKKGDLDTAAVKVEFDEEFSGAPVEGAGEEALTPSVPINKGPKPKREKKEPGTRVRKTPTSSGKPSAKKVKKRNPWSDDESKSESDLEETEPVVIPRDSLLRRAAAERPKYTFDFSEEEDDDADDDDDDNNDLEELKVKASPITNDGEDEFVPSDGLDKDEYTFSPGKSKATPEKSLHDKKSQDFGNLFSFPSYSQKSEDDSAKFDSNEEDSASVFSPSFGLKQTDKVPSKTVAAKKGKPSSDTVPKPKRAPKQKKVVEAVNSDSDSEFGIPKKTTTPKGKGRGAKKRKASGSENEGDYNPGRKTSKTTSKKPKKTSFDQDSDVDIFPSDFPTEPPSLPRTGRARKEVKYFAESDEEEDDVDFAMFN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Sheep

100

Dog

100

Bovine

Chicken

Xenopus

Pig

Horse

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q02880 As Substrate

Q02880

Site	PTM Type	Enzyme	Source
	Sumoylation		Uniprot
S4	Phosphorylation		Uniprot
K34	Sumoylation		Uniprot
K34	Ubiquitination		Uniprot
S37	Phosphorylation		Uniprot
K42	Ubiquitination		Uniprot
S45	Phosphorylation		Uniprot
S46	Phosphorylation		Uniprot
Y103	Phosphorylation		Uniprot
K117	Ubiquitination		Uniprot
K121	Ubiquitination		Uniprot
K144	Ubiquitination		Uniprot
K152	Sumoylation		Uniprot
K152	Ubiquitination		Uniprot
K155	Ubiquitination		Uniprot
Y157	Phosphorylation		Uniprot
Y172	Phosphorylation		Uniprot
K178	Ubiquitination		Uniprot
K189	Ubiquitination		Uniprot
K205	Ubiquitination		Uniprot
K212	Ubiquitination		Uniprot
K221	Ubiquitination		Uniprot
K228	Sumoylation		Uniprot
K228	Ubiquitination		Uniprot
Y235	Phosphorylation		Uniprot
K246	Ubiquitination		Uniprot
K254	Ubiquitination		Uniprot
Y265	Phosphorylation		Uniprot
K275	Acetylation		Uniprot
K282	Ubiquitination		Uniprot
Y291	Phosphorylation		Uniprot
K297	Ubiquitination		Uniprot
K299	Ubiquitination		Uniprot
K308	Ubiquitination		Uniprot
K342	Ubiquitination		Uniprot
Y349	Phosphorylation		Uniprot
K365	Acetylation		Uniprot
K367	Acetylation		Uniprot
K367	Ubiquitination		Uniprot
K373	Acetylation		Uniprot
K373	Sumoylation		Uniprot
K373	Ubiquitination		Uniprot
K407	Ubiquitination		Uniprot
K412	Methylation		Uniprot
K412	Ubiquitination		Uniprot
K418	Ubiquitination		Uniprot
K421	Methylation		Uniprot
K439	Methylation		Uniprot
K439	Ubiquitination		Uniprot
K452	Acetylation		Uniprot
Y453	Phosphorylation		Uniprot
K461	Sumoylation		Uniprot
K461	Ubiquitination		Uniprot
K471	Ubiquitination		Uniprot
K487	Ubiquitination		Uniprot
S488	Phosphorylation		Uniprot
S492	Phosphorylation		Uniprot
K510	Sumoylation		Uniprot
K510	Ubiquitination		Uniprot
K520	Ubiquitination		Uniprot
K533	Ubiquitination		Uniprot
Y539	Phosphorylation		Uniprot
K540	Ubiquitination		Uniprot
K541	Ubiquitination		Uniprot
K550	Ubiquitination		Uniprot
K556	Ubiquitination		Uniprot
K587	Methylation		Uniprot
K600	Sumoylation		Uniprot
K600	Ubiquitination		Uniprot
K605	Ubiquitination		Uniprot
K620	Ubiquitination		Uniprot
K621	Acetylation		Uniprot
K627	Ubiquitination		Uniprot
K635	Ubiquitination		Uniprot
T639	Phosphorylation		Uniprot
S640	Phosphorylation		Uniprot
K643	Sumoylation		Uniprot
K643	Ubiquitination		Uniprot
K646	Sumoylation		Uniprot
K646	Ubiquitination		Uniprot
K676	Ubiquitination		Uniprot
K683	Sumoylation		Uniprot
K683	Ubiquitination		Uniprot
R693	Methylation		Uniprot
Y707	Phosphorylation		Uniprot
K712	Ubiquitination		Uniprot
K722	Ubiquitination		Uniprot
S730	Phosphorylation		Uniprot
S735	Phosphorylation		Uniprot
S738	Phosphorylation		Uniprot
K744	Ubiquitination		Uniprot
K749	Ubiquitination		Uniprot
K756	Ubiquitination		Uniprot
K848	Ubiquitination		Uniprot
Y851	Phosphorylation		Uniprot
K884	Ubiquitination		Uniprot
Y888	Phosphorylation		Uniprot
Y913	Phosphorylation		Uniprot
Y928	Phosphorylation		Uniprot
K970	Ubiquitination		Uniprot
K979	Acetylation		Uniprot
K979	Ubiquitination		Uniprot
K988	Ubiquitination		Uniprot
K992	Acetylation		Uniprot
K992	Ubiquitination		Uniprot
K1008	Acetylation		Uniprot
K1008	Ubiquitination		Uniprot
K1032	Sumoylation		Uniprot
K1032	Ubiquitination		Uniprot
K1068	Ubiquitination		Uniprot
K1079	Ubiquitination		Uniprot
K1083	Ubiquitination		Uniprot
S1090	Phosphorylation		Uniprot
K1092	Ubiquitination		Uniprot
K1109	Ubiquitination		Uniprot
S1131	Phosphorylation		Uniprot
S1132	Phosphorylation		Uniprot
S1133	Phosphorylation		Uniprot
S1135	Phosphorylation		Uniprot
T1137	Phosphorylation		Uniprot
S1139	Phosphorylation		Uniprot
K1164	Ubiquitination		Uniprot
S1212	Phosphorylation		Uniprot
K1214	Ubiquitination		Uniprot
K1227	Sumoylation		Uniprot
K1227	Ubiquitination		Uniprot
T1233	Phosphorylation		Uniprot
S1236	Phosphorylation		Uniprot
T1247	Phosphorylation		Uniprot
K1250	Sumoylation		Uniprot
K1250	Ubiquitination		Uniprot
K1255	Acetylation		Uniprot
K1259	Acetylation		Uniprot
K1260	Acetylation		Uniprot
K1262	Ubiquitination		Uniprot
T1267	Phosphorylation		Uniprot
K1271	Sumoylation		Uniprot
S1279	Phosphorylation		Uniprot
T1292	Phosphorylation		Uniprot
K1299	Ubiquitination		Uniprot
K1302	Ubiquitination		Uniprot
K1307	Acetylation		Uniprot
T1317	Phosphorylation		Uniprot
K1323	Acetylation		Uniprot
K1323	Ubiquitination		Uniprot
K1327	Acetylation		Uniprot
K1328	Acetylation		Uniprot
S1336	Phosphorylation		Uniprot
S1340	Phosphorylation		Uniprot
S1342	Phosphorylation		Uniprot
S1344	Phosphorylation		Uniprot
T1349	Phosphorylation		Uniprot
S1358	Phosphorylation		Uniprot
R1362	Methylation		Uniprot
R1367	Methylation		Uniprot
Y1370	Phosphorylation		Uniprot
T1371	Phosphorylation		Uniprot
S1375	Phosphorylation		Uniprot
S1400	Phosphorylation		Uniprot
T1403	Phosphorylation		Uniprot
S1413	Phosphorylation		Uniprot
Y1421	Phosphorylation		Uniprot
T1422	Phosphorylation		Uniprot
S1424	Phosphorylation	P06493 (CDK1)	Uniprot
S1428	Phosphorylation		Uniprot
T1431	Phosphorylation		Uniprot
K1434	Acetylation		Uniprot
S1435	Phosphorylation		Uniprot
K1440	Sumoylation		Uniprot
K1440	Ubiquitination		Uniprot
S1441	Phosphorylation		Uniprot
S1449	Phosphorylation		Uniprot
S1452	Phosphorylation		Uniprot
Y1453	Phosphorylation		Uniprot
S1454	Phosphorylation		Uniprot
K1456	Sumoylation		Uniprot
K1456	Ubiquitination		Uniprot
S1457	Phosphorylation		Uniprot
S1461	Phosphorylation		Uniprot
S1466	Phosphorylation		Uniprot
S1471	Phosphorylation		Uniprot
S1473	Phosphorylation		Uniprot
S1476	Phosphorylation		Uniprot
K1482	Ubiquitination		Uniprot
K1486	Acetylation		Uniprot
S1501	Phosphorylation		Uniprot
S1522	Phosphorylation		Uniprot
S1524	Phosphorylation		Uniprot
S1526	Phosphorylation		Uniprot
S1550	Phosphorylation		Uniprot
S1552	Phosphorylation		Uniprot
K1574	Ubiquitination		Uniprot
T1575	Phosphorylation		Uniprot
S1576	Phosphorylation		Uniprot
S1581	Phosphorylation		Uniprot
S1588	Phosphorylation		Uniprot
T1592	Phosphorylation		Uniprot
S1596	Phosphorylation		Uniprot
T1600	Phosphorylation		Uniprot
Y1609	Phosphorylation		Uniprot
S1613	Phosphorylation		Uniprot

Research Backgrounds

Q02880_TOP2B_HUMAN

Function:

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.

Subcellular Location:

Cytoplasm. Nucleus>Nucleolus.

Subunit Structure:

Homodimer. Interacts with KIAA1210 (By similarity).

Family&Domains:

Belongs to the type II topoisomerase family.

Research Fields

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

References

1). Effect of verapamil in the reversal of doxorubicin chemotherapy resistance in advanced gastric cancer. European Review for Medical and Pharmacological Sciences, 2020 (PubMed: 32744702) [IF=3.3]

Application: WB Species: human Sample: GC cells

Figure 2. |Evaluation of candidate gene/protein expression in GC cells. A, RT-qPCR detection of candidate gene expression in GC cells. Independent experiments were performed during the whole in vitro study. B, Western blot analysis of candidate protein expression levels in GC cells.

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TOP2B Antibody - #AF0283