CCDC80 Antibody | Affinity Biosciences

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CCDC80 Antibody - DF14680 staining Hela cells by IF/ICC.

Product:	CCDC80 Antibody
Catalog:	DF14680
Description:	Rabbit polyclonal antibody to CCDC80
Application:	IF/ICC
Cited expt.:	IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	108kD(Calculated).
Uniprot:	Q76M96

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

CCDC80 Antibody detects endogenous levels of CCDC80.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

2610001E17Rik; CCD50; CCD80_HUMAN; CCDC80; CL2; Coiled coil domain containing 80; Coiled coil domain containing protein 50; Coiled-coil domain-containing protein 80; Down regulated by oncogenes protein 1; Down-regulated by oncogenes protein 1; DRO1; MGC131805; MGC134851; Nuclear envelope protein okuribin; okuribin; OTTMUSP00000030743; Protein Ymer; SSG1; steroid sensitive gene 1; steroid sensitive protein 1; Up-regulated in BRS-3 deficient mouse homolog; URB;

Immunogens

Immunogen:

A synthesized peptide derived from human CCDC80.

Uniprot:

Q76M96(CCD80_HUMAN) >>Visit HPA database.

Gene(ID):

CCDC80(151887)

Expression:

Q76M96 CCD80_HUMAN:

Expressed in dermal papilla and dermal fibroblasts (at protein level). Expressed in heart, thymus, placenta, pancreas, colon, epithelium, spleen and osteoblasts.

Sequence:

Q76M96 CCD80_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTWRMGPRFTMLLAMWLVCGSEPHPHATIRGSHGGRKVPLVSPDSSRPARFLRHTGRSRGIERSTLEEPNLQPLQRRRSVPVLRLARPTEPPARSDINGAAVRPEQRPAARGSPREMIRDEGSSARSRMLRFPSGSSSPNILASFAGKNRVWVISAPHASEGYYRLMMSLLKDDVYCELAERHIQQIVLFHQAGEEGGKVRRITSEGQILEQPLDPSLIPKLMSFLKLEKGKFGMVLLKKTLQVEERYPYPVRLEAMYEVIDQGPIRRIEKIRQKGFVQKCKASGVEGQVVAEGNDGGGGAGRPSLGSEKKKEDPRRAQVPPTRESRVKVLRKLAATAPALPQPPSTPRATTLPPAPATTVTRSTSRAVTVAARPMTTTAFPTTQRPWTPSPSHRPPTTTEVITARRPSVSENLYPPSRKDQHRERPQTTRRPSKATSLESFTNAPPTTISEPSTRAAGPGRFRDNRMDRREHGHRDPNVVPGPPKPAKEKPPKKKAQDKILSNEYEEKYDLSRPTASQLEDELQVGNVPLKKAKESKKHEKLEKPEKEKKKKMKNENADKLLKSEKQMKKSEKKSKQEKEKSKKKKGGKTEQDGYQKPTNKHFTQSPKKSVADLLGSFEGKRRLLLITAPKAENNMYVQQRDEYLESFCKMATRKISVITIFGPVNNSTMKIDHFQLDNEKPMRVVDDEDLVDQRLISELRKEYGMTYNDFFMVLTDVDLRVKQYYEVPITMKSVFDLIDTFQSRIKDMEKQKKEGIVCKEDKKQSLENFLSRFRWRRRLLVISAPNDEDWAYSQQLSALSGQACNFGLRHITILKLLGVGEEVGGVLELFPINGSSVVEREDVPAHLVKDIRNYFQVSPEYFSMLLVGKDGNVKSWYPSPMWSMVIVYDLIDSMQLRRQEMAIQQSLGMRCPEDEYAGYGYHSYHQGYQDGYQDDYRHHESYHHGYPY

Research Backgrounds

Q76M96_CCD80_HUMAN

Function:

Promotes cell adhesion and matrix assembly.

PTMs:

Phosphorylated.

Subcellular Location:

Secreted>Extracellular space>Extracellular matrix.

Tissue Specificity:

Expressed in dermal papilla and dermal fibroblasts (at protein level). Expressed in heart, thymus, placenta, pancreas, colon, epithelium, spleen and osteoblasts.

Family&Domains:

Belongs to the CCDC80 family.

References

1). Enhanced purification of uterine smooth muscle cells from adenomyosis using a novel dual-enzyme digestion method. New biotechnology, 2024 (PubMed: 39521046) [IF=4.5]

Application: IF/ICC Species: human Sample: P1 cells

Fig. 3. Tissue adhesion method generates progenitor cells that differentiate towards fibroblast-like cells. A. RNA velocity trajectory for all clusters shown by UMAP. B. Proportion of clusters in tissue adhesion and DM4 samples. C. Heatmap comparing highly expressed genes across all clusters. D. Feature plots demonstrating expression of fibroblast-related genes: FN1, COL1A2, COL3A1. E. Immunofluorescence images of CCDC80 and ACTA2 in P1 cells obtained from tissue adhesion and DM4. F. Immunofluorescence images of COL III and ACTA2 in P1 cells cultured continuously for 5 days. G. Statistical analysis of CCDC80/ACTA2 fluorescence expression ratio in P1 cells obtained from tissue adhesion and DM4 (n = 3, ns: non-significant, *P

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CCDC80 Antibody - #DF14680