FAM129B Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from various samples, using FAM129B Antibody.

Product:	FAM129B Antibody
Catalog:	DF14676
Description:	Rabbit polyclonal antibody to FAM129B
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	84kD(Calculated).
Uniprot:	Q96TA1

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

FAM129B Antibody detects endogenous levels of FAM129B.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

bA356B19.6; C9orf88; chromosome 9 open reading frame 88; DKFZP434H0820; FAM129B; family with sequence similarity 129, member B; FLJ13518; FLJ22151; FLJ22298; hypothetical protein LOC64855; Meg 3; Meg-3; Meg3; Niban like protein 1; Niban-like protein 1; NIBL1_HUMAN; OC58; OTTHUMP00000022187; OTTHUMP00000022188; Protein FAM129B;

Immunogens

Immunogen:

A synthesized peptide derived from human FAM129B.

Uniprot:

Q96TA1(NIBA2_HUMAN) >>Visit HPA database.

Gene(ID):

FAM129B(64855)

Sequence:

Q96TA1 NIBA2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGDVLSTHLDDARRQHIAEKTGKILTEFLQFYEDQYGVALFNSMRHEIEGTGLPQAQLLWRKVPLDERIVFSGNLFQHQEDSKKWRNRFSLVPHNYGLVLYENKAAYERQVPPRAVINSAGYKILTSVDQYLELIGNSLPGTTAKSGSAPILKCPTQFPLILWHPYARHYYFCMMTEAEQDKWQAVLQDCIRHCNNGIPEDSKVEGPAFTDAIRMYRQSKELYGTWEMLCGNEVQILSNLVMEELGPELKAELGPRLKGKPQERQRQWIQISDAVYHMVYEQAKARFEEVLSKVQQVQPAMQAVIRTDMDQIITSKEHLASKIRAFILPKAEVCVRNHVQPYIPSILEALMVPTSQGFTEVRDVFFKEVTDMNLNVINEGGIDKLGEYMEKLSRLAYHPLKMQSCYEKMESLRLDGLQQRFDVSSTSVFKQRAQIHMREQMDNAVYTFETLLHQELGKGPTKEELCKSIQRVLERVLKKYDYDSSSVRKRFFREALLQISIPFLLKKLAPTCKSELPRFQELIFEDFARFILVENTYEEVVLQTVMKDILQAVKEAAVQRKHNLYRDSMVMHNSDPNLHLLAEGAPIDWGEEYSNSGGGGSPSPSTPESATLSEKRRRAKQVVSVVQDEEVGLPFEASPESPPPASPDGVTEIRGLLAQGLRPESPPPAGPLLNGAPAGESPQPKAAPEASSPPASPLQHLLPGKAVDLGPPKPSDQETGEQVSSPSSHPALHTTTEDSAGVQTEF

Research Backgrounds

Q96TA1_NIBA2_HUMAN

Function:

May play a role in apoptosis suppression. May promote melanoma cell invasion in vitro.

PTMs:

Phosphorylated at Ser-641, Ser-646, Ser-692 and Ser-696 by the BRAF/MKK/ERK signaling cascade. In melanoma cells, the C-terminal phosphorylation may prevent targeting to the plasma membrane.

As apoptosis proceeds, degraded via an proteasome-independent pathway, probably by caspases.

Subcellular Location:

Cytoplasm>Cytosol. Cell junction>Adherens junction. Membrane>Lipid-anchor.
Note: In exponentially growing cells, exclusively cytoplasmic. Cell membrane localization is observed when cells reach confluency and during telophase. In melanoma cells, targeting to the plasma membrane may be impaired by C-terminal phosphorylation.

Family&Domains:

Belongs to the Niban family.

Restrictive clause

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FAM129B Antibody - #DF14676