GRF-1 Antibody - #AF6484
Product: | GRF-1 Antibody |
Catalog: | AF6484 |
Description: | Rabbit polyclonal antibody to GRF-1 |
Application: | WB IF/ICC |
Reactivity: | Human, Mouse, Rat |
Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
Mol.Wt.: | 155kDa; 171kD(Calculated). |
Uniprot: | Q9NRY4 |
RRID: | AB_2835164 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF6484, RRID:AB_2835164.
Fold/Unfold
ARHGAP35; GAP associated protein p190; Glucocorticoid receptor DNA binding factor 1; Glucocorticoid receptor DNA-binding factor 1; Glucocorticoid receptor repression factor 1; GRF 1; GRF-1; GRF1; GRLF 1; GRLF1; GRLF1_HUMAN; KIAA1722; MGC10745; p190 A; p190-A; P190A; P190A, rat, homolog of; p190ARhoGAP; p190RhoGAP; Rho GAP p190A; Rho GTPase-activating protein 35;
Immunogens
- Q9NRY4 RHG35_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MMMARKQDVRIPTYNISVVGLSGTEKEKGQCGIGKSCLCNRFVRPSADEFHLDHTSVLSTSDFGGRVVNNDHFLYWGEVSRSLEDCVECKMHIVEQTEFIDDQTFQPHRSTALQPYIKRAAATKLASAEKLMYFCTDQLGLEQDFEQKQMPDGKLLVDGFLLGIDVSRGMNRNFDDQLKFVSNLYNQLAKTKKPIVVVLTKCDEGVERYIRDAHTFALSKKNLQVVETSARSNVNVDLAFSTLVQLIDKSRGKTKIIPYFEALKQQSQQIATAKDKYEWLVSRIVKNHNENWLSVSRKMQASPEYQDYVYLEGTQKAKKLFLQHIHRLKHEHIERRRKLYLAALPLAFEALIPNLDEIDHLSCIKAKKLLETKPEFLKWFVVLEETPWDATSHIDNMENERIPFDLMDTVPAEQLYEAHLEKLRNERKRVEMRRAFKENLETSPFITPGKPWEEARSFIMNEDFYQWLEESVYMDIYGKHQKQIIDKAKEEFQELLLEYSELFYELELDAKPSKEKMGVIQDVLGEEQRFKALQKLQAERDALILKHIHFVYHPTKETCPSCPACVDAKIEHLISSRFIRPSDRNQKNSLSDPNIDRINLVILGKDGLARELANEIRALCTNDDKYVIDGKMYELSLRPIEGNVRLPVNSFQTPTFQPHGCLCLYNSKESLSYVVESIEKSRESTLGRRDNHLVHLPLTLILVNKRGDTSGETLHSLIQQGQQIASKLQCVFLDPASAGIGYGRNINEKQISQVLKGLLDSKRNLNLVSSTASIKDLADVDLRIVMCLMCGDPFSADDILFPVLQSQTCKSSHCGSNNSVLLELPIGLHKKRIELSVLSYHSSFSIRKSRLVHGYIVFYSAKRKASLAMLRAFLCEVQDIIPIQLVALTDGAVDVLDNDLSREQLTEGEEIAQEIDGRFTSIPCSQPQHKLEIFHPFFKDVVEKKNIIEATHMYDNAAEACSTTEEVFNSPRAGSPLCNSNLQDSEEDIEPSYSLFREDTSLPSLSKDHSKLSMELEGNDGLSFIMSNFESKLNNKVPPPVKPKPPVHFEITKGDLSYLDQGHRDGQRKSVSSSPWLPQDGFDPSDYAEPMDAVVKPRNEEENIYSVPHDSTQGKIITIRNINKAQSNGSGNGSDSEMDTSSLERGRKVSIVSKPVLYRTRCTRLGRFASYRTSFSVGSDDELGPIRKKEEDQASQGYKGDNAVIPYETDEDPRRRNILRSLRRNTKKPKPKPRPSITKATWESNYFGVPLTTVVTPEKPIPIFIERCIEYIEATGLSTEGIYRVSGNKSEMESLQRQFDQDHNLDLAEKDFTVNTVAGAMKSFFSELPDPLVPYNMQIDLVEAHKINDREQKLHALKEVLKKFPKENHEVFKYVISHLNKVSHNNKVNLMTSENLSICFWPTLMRPDFSTMDALTATRTYQTIIELFIQQCPFFFYNRPITEPPGARPSSPSAVASTVPFLTSTPVTSQPSPPQSPPPTPQSPMQPLLPSQLQAEHTL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NRY4 As Substrate
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S46 | Phosphorylation | Uniprot | |
Y133 | Phosphorylation | Uniprot | |
T136 | Phosphorylation | Uniprot | |
S182 | Phosphorylation | Uniprot | |
K193 | Acetylation | Uniprot | |
Y209 | Phosphorylation | Uniprot | |
S296 | Phosphorylation | Uniprot | |
Y308 | Phosphorylation | Uniprot | |
K368 | Ubiquitination | Uniprot | |
S575 | Phosphorylation | Uniprot | |
S576 | Phosphorylation | Uniprot | |
S589 | Phosphorylation | Uniprot | |
S591 | Phosphorylation | Uniprot | |
S667 | Phosphorylation | Uniprot | |
T685 | Phosphorylation | Uniprot | |
K762 | Ubiquitination | Uniprot | |
S769 | Phosphorylation | Uniprot | |
S770 | Phosphorylation | Uniprot | |
T771 | Phosphorylation | Uniprot | |
S773 | Phosphorylation | Uniprot | |
Y855 | Phosphorylation | Uniprot | |
T906 | Phosphorylation | Uniprot | |
S962 | Phosphorylation | Uniprot | |
T963 | Phosphorylation | Uniprot | |
S970 | Phosphorylation | Uniprot | |
S975 | Phosphorylation | Uniprot | |
S980 | Phosphorylation | Uniprot | |
S985 | Phosphorylation | Uniprot | |
S1001 | Phosphorylation | Uniprot | |
S1004 | Phosphorylation | Uniprot | |
S1006 | Phosphorylation | Uniprot | |
S1010 | Phosphorylation | Uniprot | |
S1023 | Phosphorylation | Uniprot | |
S1057 | Phosphorylation | Uniprot | |
S1070 | Phosphorylation | Uniprot | |
S1072 | Phosphorylation | Uniprot | |
S1073 | Phosphorylation | Uniprot | |
S1074 | Phosphorylation | Uniprot | |
S1085 | Phosphorylation | Uniprot | |
Y1087 | Phosphorylation | P06241 (FYN) | Uniprot |
K1096 | Ubiquitination | Uniprot | |
Y1105 | Phosphorylation | Q13882 (PTK6) , P12931 (SRC) , P42684 (ABL2) , P06241 (FYN) | Uniprot |
S1106 | Phosphorylation | Uniprot | |
S1111 | Phosphorylation | Uniprot | |
T1112 | Phosphorylation | Uniprot | |
K1115 | Ubiquitination | Uniprot | |
S1127 | Phosphorylation | Uniprot | |
S1130 | Phosphorylation | Uniprot | |
S1134 | Phosphorylation | Uniprot | |
S1136 | Phosphorylation | Uniprot | |
T1140 | Phosphorylation | Uniprot | |
S1141 | Phosphorylation | Uniprot | |
S1142 | Phosphorylation | Uniprot | |
S1150 | Phosphorylation | Q13464 (ROCK1) | Uniprot |
S1153 | Phosphorylation | Uniprot | |
Y1158 | Phosphorylation | Uniprot | |
T1173 | Phosphorylation | Q13464 (ROCK1) | Uniprot |
S1174 | Phosphorylation | Q13464 (ROCK1) | Uniprot |
S1176 | Phosphorylation | Uniprot | |
S1179 | Phosphorylation | Uniprot | |
S1195 | Phosphorylation | Uniprot | |
Y1198 | Phosphorylation | Uniprot | |
Y1207 | Phosphorylation | Uniprot | |
T1209 | Phosphorylation | Uniprot | |
S1221 | Phosphorylation | P17252 (PRKCA) | Uniprot |
T1226 | Phosphorylation | Q13464 (ROCK1) , P17252 (PRKCA) | Uniprot |
S1236 | Phosphorylation | Q13464 (ROCK1) , P17252 (PRKCA) | Uniprot |
K1289 | Ubiquitination | Uniprot | |
S1290 | Phosphorylation | Uniprot | |
K1353 | Ubiquitination | Uniprot | |
K1358 | Ubiquitination | Uniprot | |
K1381 | Ubiquitination | Uniprot | |
S1450 | Phosphorylation | Uniprot | |
S1476 | Phosphorylation | Uniprot | |
T1480 | Phosphorylation | Uniprot | |
S1491 | Phosphorylation | Uniprot |
Research Backgrounds
Rho GTPase-activating protein (GAP). Binds several acidic phospholipids which inhibits the Rho GAP activity to promote the Rac GAP activity. This binding is inhibited by phosphorylation by PRKCA. Involved in cell differentiation as well as cell adhesion and migration, plays an important role in retinal tissue morphogenesis, neural tube fusion, midline fusion of the cerebral hemispheres and mammary gland branching morphogenesis (By similarity). Transduces signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP) (By similarity). Transduces SRC-dependent signals from cell-surface adhesion molecules, such as laminin, to promote neurite outgrowth. Regulates axon outgrowth, guidance and fasciculation (By similarity). Modulates Rho GTPase-dependent F-actin polymerization, organization and assembly, is involved in polarized cell migration and in the positive regulation of ciliogenesis and cilia elongation (By similarity). During mammary gland development, is required in both the epithelial and stromal compartments for ductal outgrowth (By similarity). Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'; this function is however unclear and would need additional experimental evidences.
Phosphorylation of Tyr-1105 by PTK6 promotes the association with RASA1, inactivating RHOA while activating RAS. Phosphorylation at Tyr-308 by PDGFRA inhibits binding to GTF2I. Phosphorylated by PRKCA at Ser-1221 and Thr-1226, induces relocalization from the cytoplasm to regions of plasma membrane ruffling and prevents the binding and substrate specificity regulation by phospholipids. In brain, phosphorylated by FYN and SRC (By similarity). During focal adhesion formation, phosphorylated by MAPK1 and MAPK3 at the C-terminal region, probably at Ser-1451, Ser-1476, Thr-1480 and Ser-1483. Phosphorylation by MAPK1 and MAPK3 inhibits GAP function and localizes ARGHAP35 away from newly forming focal adhesions and stress fibers in cells spreading on fibronectin (By similarity). Phosphorylation at Ser-1476 and Thr-1480 by GSK3B requires priming by MAPK and inhibits RhoGAP activity and modulates polarized cell migration (By similarity).
Cytoplasm>Cytoskeleton>Cilium basal body. Cytoplasm. Nucleus. Cell membrane.
Note: In response to integrins and SDC4 and upon phosphorylation by PKC, relocalizes from the cytoplasm to regions of plasma membrane ruffling where it colocalizes with polymerized actin.
Detected in neutrophils (at protein level).
Interacts with RASA1 (By similarity). Interacts with the general transcription factor GTF2I, the interaction sequesters GTF2I in the cytoplasm.
N-terminal part (1-266) has GTPase activity. Required for proper cellular localization.
The pG1 pseudoGTPase domain does not bind GTP.
Research Fields
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Organismal Systems > Immune system > Platelet activation. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.