Sumo1 Antibody - #AF0280

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Product: Sumo1 Antibody
Catalog: AF0280
Description: Rabbit polyclonal antibody to Sumo1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Dog, Chicken
Mol.Wt.: 12kDa; 12kD(Calculated).
Uniprot: P63165
RRID: AB_2833449

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
Sumo1 Antibody detects endogenous levels of total Sumo1.
RRID:
AB_2833449
Cite Format: Affinity Biosciences Cat# AF0280, RRID:AB_2833449.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DAP1; GAP modifying protein 1; GAP-modifying protein 1; GMP 1; GMP1; OFC10; PIC 1; PIC1; SENP2; Sentrin 1; Sentrin; Small ubiquitin related modifier 1; Small ubiquitin-like modifier 1; Small ubiquitin-related modifier 1; SMT3; SMT3 homolog 3; SMT3 suppressor of mif two 3 homolog 1; SMT3, yeast, homolog 3; Smt3C; SMT3H3; SUMO-1; SUMO1; SUMO1_HUMAN; Ubiquitin homology domain protein PIC1; Ubiquitin Like 1; Ubiquitin like protein SMT3C; Ubiquitin like protein UBL1; Ubiquitin-homology domain protein PIC1; Ubiquitin-like protein SMT3C; Ubiquitin-like protein UBL1; UBL 1; UBL1;

Immunogens

Immunogen:
Uniprot:

P63165(SUMO1_HUMAN) >>Visit HPA database.

Gene(ID):
SUMO1(7341)
Description:
SUMO1 Ubiquitin-like protein which can be covalently attached to target lysines as a monomer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction.
Sequence:
MSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGHSTV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Chicken
100
Zebrafish
92
Sheep
0
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P63165 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
K7 Sumoylation
K7 Ubiquitination
S9 Phosphorylation
T10 Phosphorylation
K16 Sumoylation
K16 Ubiquitination
K17 Sumoylation
K17 Ubiquitination
K23 Acetylation
K23 Sumoylation
K23 Ubiquitination
K25 Sumoylation
K25 Ubiquitination
S32 Phosphorylation
K37 Acetylation
K37 Methylation
K37 Sumoylation
K37 Ubiquitination
K39 Methylation
K39 Sumoylation
K39 Ubiquitination
K45 Sumoylation
K45 Ubiquitination
K46 Sumoylation
K46 Ubiquitination
K48 Sumoylation
K48 Ubiquitination
R63 Methylation
R70 Methylation
T76 Phosphorylation
K78 Sumoylation
K78 Ubiquitination

Research Backgrounds

Function:

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Covalently attached to the voltage-gated potassium channel KCNB1; this modulates the gating characteristics of KCNB1. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development. Covalently attached to ZFHX3.

PTMs:

Cleavage of precursor form by SENP1 or SENP2 is necessary for function.

Polymeric SUMO1 chains undergo polyubiquitination by RNF4.

Subcellular Location:

Nucleus membrane. Nucleus speckle. Cytoplasm. Nucleus>PML body. Cell membrane. Nucleus.
Note: Recruited by BCL11A into the nuclear body. In the presence of ZFHX3, sequesterd to nuclear body (NB)-like dots in the nucleus some of which overlap or closely associate with PML body.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Covalently attached to KCNB1; UBE2I increases cross-linking with KCNB1 and PIAS1 decreases cross-links with KCNB1. Interacts with SAE2, RANBP2, PIAS1 and PIAS2. Interacts with PRKN. Covalently attached to a number of proteins such as IKFZ1, PML, RANGAP1, HIPK2, SP100, p53, p73-alpha, MDM2, JUN, DNMT3B and TDG. Also interacts with HIF1A, HIPK2, HIPK3, CHD3, EXOSC9, RAD51 and RAD52. Interacts with USP25 (via ts SIM domain); the interaction weakly sumoylates USP25. Interacts with SIMC1, CASP8AP2, RNF111 AND SOBP (via SIM domains). Interacts with BHLHE40/DEC1. Interacts with RWDD3. Interacts with UBE2I/UBC9 and this interaction is enhanced in the presence of RWDD3. Interacts with MTA1.

(Microbial infection) Interacts with Epstein-barr virus BGLF4.

Family&Domains:

Belongs to the ubiquitin family. SUMO subfamily.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

References

1). SUMOylation of Vps34 by SUMO1 promotes phenotypic switching of vascular smooth muscle cells by activating autophagy in pulmonary arterial hypertension. PULMONARY PHARMACOLOGY & THERAPEUTICS, 2019 (PubMed: 30703554) [IF=3.2]

Application: WB    Species: mouse    Sample: lung

Fig. 2 |Association of increased SUMO1 expression with activation of autophagy in PAH.(A) Western blot analysis using lung tissue samples showed significantly increased expression levels of SUMO1, HIF-1α and autophagy marker LC3b, and a significantly decreased expression level of autophagy marker p62 in hypoxic PAH mice than in control mice. (B) The Western blotting data in (A) were quantified and plotted. (n=6/group,*P<0.05, **P<0.01).

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