DKK4 Antibody - #DF14554

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Product: DKK4 Antibody
Catalog: DF14554
Description: Rabbit polyclonal antibody to DKK4
Application: IHC IF/ICC
Reactivity: Human
Mol.Wt.: 25kD(Calculated).
Uniprot: Q9UBT3

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
DKK4 Antibody detects endogenous levels of DKK4.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Dickkopf 4; Dickkopf gene 4; Dickkopf homolog 4 (Xenopus laevis); Dickkopf homolog 4; Dickkopf related protein 4; Dickkopf-4; Dickkopf-related protein 4 short form; Dickkopf4; DKK 4; Dkk-4; DKK4; DKK4_HUMAN; hDkk 4; hDkk-4; hDkk4; MGC129562; MGC129563;

Immunogens

Immunogen:

A synthesized peptide derived from human DKK4.

Uniprot:

Q9UBT3(DKK4_HUMAN) >>Visit HPA database.

Gene(ID):
DKK4(27121)
Expression:
Q9UBT3 DKK4_HUMAN:

Expressed in cerebellum, T-cells, esophagus and lung.

Sequence:
MVAAVLLGLSWLCSPLGALVLDFNNIRSSADLHGARKGSQCLSDTDCNTRKFCLQPRDEKPFCATCRGLRRRCQRDAMCCPGTLCVNDVCTTMEDATPILERQLDEQDGTHAEGTTGHPVQENQPKRKPSIKKSQGRKGQEGESCLRTFDCGPGLCCARHFWTKICKPVLLEGQVCSRRGHKDTAQAPEIFQRCDCGPGLLCRSQLTSNRQHARLRVCQKIEKL

Research Backgrounds

Function:

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity).

PTMs:

Appears to be not glycosylated.

Can be proteolytically processed by a furin-like protease.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in cerebellum, T-cells, esophagus and lung.

Subunit Structure:

Interacts with LRP5 and LRP6.

Family&Domains:

The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.

Belongs to the dickkopf family.

Research Fields

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

Restrictive clause

 

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