HNF4 alpha Antibody | Affinity Biosciences

Product:	HNF4 alpha Antibody
Catalog:	AF6297
Description:	Rabbit polyclonal antibody to HNF4 alpha
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	52kDa; 53kD(Calculated).
Uniprot:	P41235
RRID:	AB_2835147

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(82%), Xenopus(82%)

Clonality:

Polyclonal

Specificity:

HNF4 alpha Antibody detects endogenous levels of total HNF4 alpha.

RRID:

AB_2835147
Cite Format: Affinity Biosciences Cat# AF6297, RRID:AB_2835147.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FLJ39654; FRTS4; Hepatic nuclear factor 4 alpha; Hepatocyte nuclear factor 4 alpha; Hepatocyte nuclear factor 4; Hepatocyte nuclear factor 4-alpha; HNF 4 alpha; HNF 4; HNF-4-alpha; HNF4; HNF4A; HNF4A_HUMAN; HNF4a7; HNF4a8; HNF4a9; Hnf4alpha; HNF4alpha10/11/12; MODY 1; MODY; MODY1; NR2A1; NR2A21; Nuclear receptor subfamily 2 group A member 1; OTTHUMP00000031060; OTTHUMP00000031062; TCF 14; TCF; TCF-14; TCF14; Tcf4; Transcription factor 14, hepatic nuclear factor; Transcription factor 14; Transcription factor HNF 4; Transcription factor HNF-4; Transcription factor HNF4;

Immunogens

Immunogen:

Uniprot:

P41235(HNF4A_HUMAN) >>Visit HPA database.

Gene(ID):

HNF4A(3172)

Description:

The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes.

Sequence:

P41235 HNF4A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRLSKTLVDMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGTNLNAPNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSRQITSPVSGINGDIRAKKIASIADVCESMKEQLLVLVEWAKYIPAFCELPLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQIDDNEYAYLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFIKLFGMAKIDNLLQEMLLGGSPSDAPHAHHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMCEWPRPRGQAATPETPQPSPPGGSGSEPYKLLPGAVATIVKPLSAIPQPTITKQEVI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P41235 As Substrate

P41235

Site	PTM Type	Enzyme	Source
Y23	Phosphorylation	P12931 (SRC)	Uniprot
Y72	Phosphorylation		Uniprot
S75	Phosphorylation		Uniprot
S76	Phosphorylation		Uniprot
S95	Phosphorylation		Uniprot
S99	Phosphorylation		Uniprot
K106	Acetylation		Uniprot
K108	Acetylation		Uniprot
K126	Acetylation		Uniprot
K127	Acetylation		Uniprot
S138	Phosphorylation		Uniprot
T139	Phosphorylation		Uniprot
S142	Phosphorylation	P17612 (PRKACA)	Uniprot
S143	Phosphorylation	P17612 (PRKACA)	Uniprot
Y144	Phosphorylation		Uniprot
S147	Phosphorylation		Uniprot
S148	Phosphorylation		Uniprot
S151	Phosphorylation		Uniprot
T166	Phosphorylation		Uniprot
S167	Phosphorylation	Q16539 (MAPK14) , Q15759 (MAPK11)	Uniprot
S170	Phosphorylation		Uniprot
S190	Phosphorylation		Uniprot
K234	Ubiquitination		Uniprot
S262	Phosphorylation		Uniprot
S265	Phosphorylation		Uniprot
Y286	Phosphorylation	P12931 (SRC)	Uniprot
Y288	Phosphorylation	P12931 (SRC)	Uniprot
S303	Phosphorylation	Q13131 (PRKAA1)	Uniprot
K307	Ubiquitination		Uniprot
K309	Ubiquitination		Uniprot
S313	Phosphorylation	Q13131 (PRKAA1)	Uniprot
S318	Phosphorylation		Uniprot
S378	Phosphorylation		Uniprot
T429	Phosphorylation		Uniprot
T432	Phosphorylation		Uniprot
S436	Phosphorylation		Uniprot
S441	Phosphorylation		Uniprot
S443	Phosphorylation		Uniprot
T455	Phosphorylation		Uniprot
K458	Acetylation		Uniprot
S461	Phosphorylation		Uniprot
T467	Phosphorylation		Uniprot
T469	Phosphorylation		Uniprot

Research Backgrounds

P41235_HNF4A_HUMAN

Function:

Transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitating the recruitment of RNA pol II to the promoters of target genes. Activates the transcription of CYP2C38 (By similarity). Represses the CLOCK-ARNTL/BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells.

PTMs:

Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA.

Acetylation at Lys-458 lowers transcriptional activation by about two-fold.

Subcellular Location:

Nucleus.

Subunit Structure:

Homodimerization is required for HNF4-alpha to bind to its recognition site. Interacts with CLOCK, ARNTL, CRY1, CRY2, PER1 and PER2. Interacts with NR0B2/SHP; the resulting heterodimer is transcriptionnally inactive. Interacts with DDX3X; this interaction disrupts the interaction between HNF4 and NR0B2 that forms inactive heterodimers and enhances the formation of active HNF4 homodimers.

Family&Domains:

The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Belongs to the nuclear hormone receptor family. NR2 subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.

Restrictive clause

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For Research Use Only.
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HNF4 alpha Antibody - #AF6297