Ribonuclease 7 Antibody - #DF14437

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Product: Ribonuclease 7 Antibody
Catalog: DF14437
Description: Rabbit polyclonal antibody to Ribonuclease 7
Application: IHC IF/ICC
Reactivity: Human
Mol.Wt.: 17kD(Calculated).
Uniprot: Q9H1E1

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
Ribonuclease 7 Antibody detects endogenous levels of total Ribonuclease 7.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EC 3.1.27.-; MGC133220; Ribonuclease 7; ribonuclease, RNase A family, 7; RNAS7_HUMAN; RNase 7; RNASE7; SAP 2; SAP-2; Skin derived antimicrobial protein 2; Skin-derived antimicrobial protein 2;

Immunogens

Immunogen:

A synthesized peptide derived from Human Ribonuclease 7.

Uniprot:

Q9H1E1(RNAS7_HUMAN) >>Visit HPA database.

Gene(ID):
RNASE7(84659)
Expression:
Q9H1E1 RNAS7_HUMAN:

Expressed in collecting ducts in kidney, and in apical uroepithelium in bladder (at protein level) (PubMed:25075772). Expressed in various epithelial tissues including skin, respiratory tract, genito-urinary tract and, at a low level, in the gut (PubMed:12244054). Expressed in liver, kidney, skeletal muscle and heart (PubMed:12527768).

Sequence:
MAPARAGFCPLLLLLLLGLWVAEIPVSAKPKGMTSSQWFKIQHMQPSPQACNSAMKNINKHTKRCKDLNTFLHEPFSSVAATCQTPKIACKNGDKNCHQSHGAVSLTMCKLTSGKHPNCRYKEKRQNKSYVVACKPPQKKDSQQFHLVPVHLDRVL

Research Backgrounds

Function:

Exhibits a potent RNase activity. Has broad-spectrum antimicrobial activity against many pathogenic microorganisms and remarkably potent activity (lethal dose of 90% < 30 nM) against a vancomycin resistant Enterococcus faecium. Causes loss of bacterial membrane integrity. Probably contributes to urinary tract sterility. Bactericidal activity is independent of RNase activity.

Subcellular Location:

Secreted.
Note: Detected in urine.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in collecting ducts in kidney, and in apical uroepithelium in bladder (at protein level). Expressed in various epithelial tissues including skin, respiratory tract, genito-urinary tract and, at a low level, in the gut. Expressed in liver, kidney, skeletal muscle and heart.

Family&Domains:

Belongs to the pancreatic ribonuclease family.

References

1). Increased S. Aureus Colonization and Reduced Antimicrobial Peptide Expression in Erythrodermic Psoriasis. International immunopharmacology, 2024 (PubMed: 38096593) [IF=5.6]

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