Legumain Antibody | Affinity Biosciences

IF/ICC

1/1

Product:	Legumain Antibody
Catalog:	DF14435
Description:	Rabbit polyclonal antibody to Legumain
Application:	IF/ICC
Reactivity:	Human
Mol.Wt.:	49kD(Calculated).
Uniprot:	Q99538

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Secondary Antibodies
Blocking Peptides

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

Legumain Antibody detects endogenous levels of total Legumain.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AEP; Asparaginyl endopeptidase; cysteine 1; Cysteine protease 1; EC 3.4.22.34; Legumain; LGMN; LGMN_HUMAN; LGMN1; Protease; Protease cysteine 1 (legumain); Protease cysteine 1; PRSC1;

Immunogens

Immunogen:

A synthesized peptide derived from Human Legumain.

Uniprot:

Q99538(LGMN_HUMAN) >>Visit HPA database.

Gene(ID):

LGMN(5641)

Expression:

Q99538 LGMN_HUMAN:

Ubiquitous. Particularly abundant in kidney, heart and placenta.

Sequence:

Q99538 LGMN_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVWKVAVFLSVALGIGAVPIDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQIVVMMYDDIAYSEDNPTPGIVINRPNGTDVYQGVPKDYTGEDVTPQNFLAVLRGDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPNEDLHVKDLNETIHYMYKHKMYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKRSTYLGDWYSVNWMEDSDVEDLTKETLHKQYHLVKSHTNTSHVMQYGNKTISTMKVMQFQGMKRKASSPVPLPPVTHLDLTPSPDVPLTIMKRKLMNTNDLEESRQLTEEIQRHLDARHLIEKSVRKIVSLLAASEAEVEQLLSERAPLTGHSCYPEALLHFRTHCFNWHSPTYEYALRHLYVLVNLCEKPYPLHRIKLSMDHVCLGHY

Research Backgrounds

Q99538_LGMN_HUMAN

Function:

Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation (By similarity). May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.

PTMs:

Glycosylated.

Activated by autocatalytic processing at pH 4.

Subcellular Location:

Lysosome.

Tissue Specificity:

Ubiquitous. Particularly abundant in kidney, heart and placenta.

Subunit Structure:

Homodimer before autocatalytic removal of the propeptide (By similarity). Monomer after autocatalytic processing. May interact with integrins.

Family&Domains:

In the zymogen form, the uncleaved propeptide blocks access to the active site.

Belongs to the peptidase C13 family.

Research Fields

· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)

· Organismal Systems > Immune system > Antigen processing and presentation. (View pathway)

Restrictive clause

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For Research Use Only.
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Legumain Antibody - #DF14435