CD5 Antibody | Affinity Biosciences

IF/ICC

1/1

Product:	CD5 Antibody
Catalog:	DF14431
Description:	Rabbit polyclonal antibody to CD5
Application:	IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	55kD(Calculated).
Uniprot:	P06127

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Secondary Antibodies
Blocking Peptides

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

CD5 Antibody detects endogenous levels of total CD5.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CD 5; CD5; CD5 antigen (p56 62); CD5 antigen; CD5 molecule; CD5_HUMAN; LEU1; LyA; Lymphocyte antigen T1/Leu-1; OTTHUMP00000236973; T-cell surface glycoprotein CD5; T1;

Immunogens

Immunogen:

A synthesized peptide derived from Human CD5.

Uniprot:

P06127(CD5_HUMAN) >>Visit HPA database.

Gene(ID):

CD5(921)

Sequence:

P06127 CD5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPMGSLQPLATLYLLGMLVASCLGRLSWYDPDFQARLTRSNSKCQGQLEVYLKDGWHMVCSQSWGRSSKQWEDPSQASKVCQRLNCGVPLSLGPFLVTYTPQSSIICYGQLGSFSNCSHSRNDMCHSLGLTCLEPQKTTPPTTRPPPTTTPEPTAPPRLQLVAQSGGQHCAGVVEFYSGSLGGTISYEAQDKTQDLENFLCNNLQCGSFLKHLPETEAGRAQDPGEPREHQPLPIQWKIQNSSCTSLEHCFRKIKPQKSGRVLALLCSGFQPKVQSRLVGGSSICEGTVEVRQGAQWAALCDSSSARSSLRWEEVCREQQCGSVNSYRVLDAGDPTSRGLFCPHQKLSQCHELWERNSYCKKVFVTCQDPNPAGLAAGTVASIILALVLLVVLLVVCGPLAYKKLVKKFRQKKQRQWIGPTGMNQNMSFHRNHTATVRSHAENPTASHVDNEYSQPPRNSHLSAYPALEGALHRSSMQPDNSSDSDYDLHGAQRL

Research Backgrounds

P06127_CD5_HUMAN

Function:

May act as a receptor in regulating T-cell proliferation.

PTMs:

Phosphorylated on tyrosine residues by LYN; this creates binding sites for PTPN6/SHP-1.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Subunit Structure:

Interacts with CD72/LYB-2. Interacts with PTPN6/SHP-1 (By similarity).

Research Fields

· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

CD5 Antibody - #DF14431