PPP2R5B Antibody - #DF14410

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Product: PPP2R5B Antibody
Catalog: DF14410
Description: Rabbit polyclonal antibody to PPP2R5B
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 57kD(Calculated).
Uniprot: Q15173

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
PPP2R5B Antibody detects endogenous levels of total PPP2R5B.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2A5B_HUMAN; B56B; Beta isoform of regulatory subunit B56 protein phosphatase 2A; FLJ35411; PP2A B subunit B' beta isoform; PP2A B subunit B56 beta isoform; PP2A B subunit isoform B' beta; PP2A B subunit isoform B''-beta; PP2A B subunit isoform B56-beta; PP2A B subunit isoform PR61-beta; PP2A B subunit isoform R5-beta; PP2A B subunit PR61 beta isoform; PP2A B subunit R5 beta isoform; PPP2R5B; PR 61B; PR61B; Protein phosphatase 2 regulatory subunit B (B56) beta; Protein phosphatase 2 regulatory subunit B (B56) beta isoform; Protein phosphatase 2 regulatory subunit B' beta; Protein phosphatase 2 regulatory subunit B' beta isoform; Serine/threonine protein phosphatase 2A 56 kDa regulatory subunit beta isoform; Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform;

Immunogens

Immunogen:

A synthesized peptide derived from Human PPP2R5B.

Uniprot:

Q15173(2A5B_HUMAN) >>Visit HPA database.

Gene(ID):
PPP2R5B(5526)
Expression:
Q15173 2A5B_HUMAN:

Highest expression in brain.

Sequence:
METKLPPASTPTSPSSPGLSPVPPPDKVDGFSRRSLRRARPRRSHSSSQFRYQSNQQELTPLPLLKDVPASELHELLSRKLAQCGVMFDFLDCVADLKGKEVKRAALNELVECVGSTRGVLIEPVYPDIIRMISVNIFRTLPPSENPEFDPEEDEPNLEPSWPHLQLVYEFFLRFLESPDFQPSVAKRYVDQKFVLMLLELFDSEDPREREYLKTILHRVYGKFLGLRAYIRKQCNHIFLRFIYEFEHFNGVAELLEILGSIINGFALPLKTEHKQFLVRVLIPLHSVKSLSVFHAQLAYCVVQFLEKDATLTEHVIRGLLKYWPKTCTQKEVMFLGEMEEILDVIEPSQFVKIQEPLFKQVARCVSSPHFQVAERALYFWNNEYILSLIEDNCHTVLPAVFGTLYQVSKEHWNQTIVSLIYNVLKTFMEMNGKLFDELTASYKLEKQQEQQKAQERQELWQGLEELRLRRLQGTQGAKEAPLQRLTPQVAASGGQS

Research Backgrounds

Function:

As the regulatory component of the serine/threonine-protein phosphatase 2A (PP2A) holoenzyme, modulates substrate specificity, subcellular localization, and responsiveness to phosphorylation. The phosphorylated form mediates the interaction between PP2A and AKT1, leading to AKT1 dephosphorylation.

PTMs:

Ubiquitinated by E3 CUL3-KLHL15 complex; this modification leads to proteasomal degradation.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highest expression in brain.

Subunit Structure:

Component of the serine/threonine-protein phosphatase 2A complex (PP2A). This complex consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant scaffold subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with SGO1. Interacts with AKT1. Interacts with CUL3 and KLHL15; this interaction leads to proteasomal degradation.

Family&Domains:

Belongs to the phosphatase 2A regulatory subunit B56 family.

Research Fields

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Genetic Information Processing > Translation > mRNA surveillance pathway.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Nervous system > Dopaminergic synapse.

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