ADD1 Antibody - #AF6276

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Product: ADD1 Antibody
Catalog: AF6276
Description: Rabbit polyclonal antibody to ADD1
Application: WB IHC IF/ICC IP
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Zebrafish, Bovine, Horse, Dog, Xenopus
Mol.Wt.: 80kDa; 81kD(Calculated).
Uniprot: P35611 | P35612
RRID: AB_2835129

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 100ul $280 In stock
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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IP, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Zebrafish(100%), Bovine(100%), Horse(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
ADD1 Antibody detects endogenous levels of total ADD1.
RRID:
AB_2835129
Cite Format: Affinity Biosciences Cat# AF6276, RRID:AB_2835129.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ADD A; ADD1; ADDA; ADDA_HUMAN; Adducin 1 (alpha); Alpha adducin; Alpha-adducin; Erythrocyte adducin alpha subunit; Erythrocyte adducin subunit alpha; MGC3339; MGC44427; OTTHUMP00000151163; OTTHUMP00000151164; OTTHUMP00000151165; OTTHUMP00000218664; OTTHUMP00000218665; ADD1; ADD2; ADD3; ADDA; ADDB; ADDL; Adducin 1 (alpha); Adducin 1; Adducin 2 (beta); Adducin 2; Adducin 3 (gamma); Adducin 3; Adducin like; Alpha adducin; Beta adducin;

Immunogens

Immunogen:
Uniprot:

P35611(ADDA_HUMAN) >>Visit HPA database.

P35612(ADDB_HUMAN) >>Visit HPA database.

Gene(ID):
ADD1(118) | ADD2(119)
Expression:
P35611 ADDA_HUMAN:

Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.

P35612 ADDB_HUMAN:

Expressed mainly in brain, spleen, kidney cortex and medulla, and heart. Also expressed in human umbilical vein endothelial cells, human vascular smooth muscle cells, kidney tubular cells and K-562 cell line.

Description:
ADD2 a cytoskeletal protein that promotes the assembly of the spectrin-actin network. Adducin is a heterodimeric protein that consists of related subunits. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region.
Sequence:
MNGDSRAAVVTSPPPTTAPHKERYFDRVDENNPEYLRERNMAPDLRQDFNMMEQKKRVSMILQSPAFCEELESMIQEQFKKGKNPTGLLALQQIADFMTTNVPNVYPAAPQGGMAALNMSLGMVTPVNDLRGSDSIAYDKGEKLLRCKLAAFYRLADLFGWSQLIYNHITTRVNSEQEHFLIVPFGLLYSEVTASSLVKINLQGDIVDRGSTNLGVNQAGFTLHSAIYAARPDVKCVVHIHTPAGAAVSAMKCGLLPISPEALSLGEVAYHDYHGILVDEEEKVLIQKNLGPKSKVLILRNHGLVSVGESVEEAFYYIHNLVVACEIQVRTLASAGGPDNLVLLNPEKYKAKSRSPGSPVGEGTGSPPKWQIGEQEFEALMRMLDNLGYRTGYPYRYPALREKSKKYSDVEVPASVTGYSFASDGDSGTCSPLRHSFQKQQREKTRWLNSGRGDEASEEGQNGSSPKSKTKWTKEDGHRTSTSAVPNLFVPLNTNPKEVQEMRNKIREQNLQDIKTAGPQSQVLCGVVMDRSLVQGELVTASKAIIEKEYQPHVIVSTTGPNPFTTLTDRELEEYRREVERKQKGSEENLDEAREQKEKSPPDQPAVPHPPPSTPIKLEEDLVPEPTTGDDSDAATFKPTLPDLSPDEPSEALGFPMLEKEEEAHRPPSPTEAPTEASPEPAPDPAPVAEEAAPSAVEEGAAADPGSDGSPGKSPSKKKKKFRTPSFLKKSKKKSDS

MSEETVPEAASPPPPQGQPYFDRFSEDDPEYMRLRNRAADLRQDFNLMEQKKRVTMILQSPSFREELEGLIQEQMKKGNNSSNIWALRQIADFMASTSHAVFPTSSMNVSMMTPINDLHTADSLNLAKGERLMRCKISSVYRLLDLYGWAQLSDTYVTLRVSKEQDHFLISPKGVSCSEVTASSLIKVNILGEVVEKGSSCFPVDTTGFCLHSAIYAARPDVRCIIHLHTPATAAVSAMKWGLLPVSHNALLVGDMAYYDFNGEMEQEADRINLQKCLGPTCKILVLRNHGVVALGDTVEEAFYKIFHLQAACEIQVSALSSAGGVENLILLEQEKHRPHEVGSVQWAGSTFGPMQKSRLGEHEFEALMRMLDNLGYRTGYTYRHPFVQEKTKHKSEVEIPATVTAFVFEEDGAPVPALRQHAQKQQKEKTRWLNTPNTYLRVNVADEVQRSMGSPRPKTTWMKADEVEKSSSGMPIRIENPNQFVPLYTDPQEVLEMRNKIREQNRQDVKSAGPQSQLLASVIAEKSRSPSTESQLMSKGDEDTKDDSEETVPNPFSQLTDQELEEYKKEVERKKLELDGEKETAPEEPGSPAKSAPASPVQSPAKEAETKSPLVSPSKSLEEGTKKTETSKAATTEPETTQPEGVVVNGREEEQTAEEILSKGLSQMTTSADTDVDTSKDKTESVTSGPMSPEGSPSKSPSKKKKKFRTPSFLKKSKKKEKVES

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Xenopus
100
Zebrafish
100
Pig
0
Sheep
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35611/P35612 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S5 Phosphorylation
T11 Phosphorylation
S12 Phosphorylation P06493 (CDK1)
T16 Phosphorylation
K21 Ubiquitination
Y24 Phosphorylation
R27 Methylation
Y35 Phosphorylation
K55 Ubiquitination
S59 Phosphorylation
S64 Phosphorylation
K140 Acetylation
K140 Ubiquitination
K143 Ubiquitination
K148 Ubiquitination
K288 Ubiquitination
T331 Phosphorylation
S334 Phosphorylation
K348 Ubiquitination
S353 Phosphorylation
S355 Phosphorylation P06493 (CDK1)
S358 Phosphorylation
T364 Phosphorylation
S366 Phosphorylation
Y393 Phosphorylation
Y395 Phosphorylation
K406 Ubiquitination
S408 Phosphorylation P17612 (PRKACA)
S423 Phosphorylation
S427 Phosphorylation
T429 Phosphorylation
S431 Phosphorylation
S436 Phosphorylation P17612 (PRKACA)
K439 Ubiquitination
T445 Phosphorylation Q9Y5S2 (CDC42BPB) , Q13464 (ROCK1) , O75116 (ROCK2)
S457 Phosphorylation
S464 Phosphorylation
S465 Phosphorylation
T480 Phosphorylation Q13464 (ROCK1) , O75116 (ROCK2)
S481 Phosphorylation P17612 (PRKACA)
T482 Phosphorylation
S483 Phosphorylation
K515 Ubiquitination
C525 S-Nitrosylation
R531 Methylation
S532 Phosphorylation
Y550 Phosphorylation
T559 Phosphorylation
Y575 Phosphorylation
S586 Phosphorylation
S600 Phosphorylation
S613 Phosphorylation P24941 (CDK2)
T614 Phosphorylation P24941 (CDK2)
S645 Phosphorylation
S669 Phosphorylation
T671 Phosphorylation
S678 Phosphorylation
S707 Phosphorylation
S710 Phosphorylation
S714 Phosphorylation
S716 Phosphorylation P17252 (PRKCA)
T724 Phosphorylation
S726 Phosphorylation Q05513 (PRKCZ) , O14757 (CHEK1) , P17252 (PRKCA) , Q05655 (PRKCD) , P17612 (PRKACA)
S731 Phosphorylation
S735 Phosphorylation
S737 Phosphorylation
Site PTM Type Enzyme
S2 Phosphorylation
T5 Phosphorylation
S11 Phosphorylation
Y31 Phosphorylation
T55 Phosphorylation
S60 Phosphorylation
S62 Phosphorylation
Y377 Phosphorylation
S396 Phosphorylation
Y440 Phosphorylation
S455 Phosphorylation
K464 Acetylation
S472 Phosphorylation
Y489 Phosphorylation P06241 (FYN)
S512 Phosphorylation
S522 Phosphorylation
S528 Phosphorylation
S530 Phosphorylation
S532 Phosphorylation
T533 Phosphorylation
S535 Phosphorylation
K576 Acetylation
K583 Acetylation
T585 Phosphorylation
S592 Phosphorylation
K595 Acetylation
S596 Phosphorylation
S600 Phosphorylation
S604 Phosphorylation Q00535 (CDK5)
T611 Phosphorylation
S613 Phosphorylation P49841 (GSK3B)
S617 Phosphorylation Q00535 (CDK5)
S619 Phosphorylation
S621 Phosphorylation
T626 Phosphorylation
T631 Phosphorylation
S632 Phosphorylation
T675 Phosphorylation
K681 Acetylation
K683 Acetylation
T684 Phosphorylation
S693 Phosphorylation P49841 (GSK3B)
S697 Phosphorylation
S699 Phosphorylation
S701 Phosphorylation Q00535 (CDK5)
S703 Phosphorylation
T711 Phosphorylation
S713 Phosphorylation O14757 (CHEK1) , P17612 (PRKACA) , Q05513 (PRKCZ) , P17252 (PRKCA)
S718 Phosphorylation
S726 Phosphorylation P17252 (PRKCA)

Research Backgrounds

Function:

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cell membrane>Peripheral membrane protein>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.

Subunit Structure:

Heterodimer of an alpha and a beta subunit or an alpha and a gamma subunit.

Family&Domains:

Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.

Belongs to the aldolase class II family. Adducin subfamily.

Function:

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to the erythrocyte membrane receptor SLC2A1/GLUT1 and may therefore provide a link between the spectrin cytoskeleton to the plasma membrane. Binds to calmodulin. Calmodulin binds preferentially to the beta subunit.

PTMs:

The N-terminus is blocked.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cell membrane>Peripheral membrane protein>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed mainly in brain, spleen, kidney cortex and medulla, and heart. Also expressed in human umbilical vein endothelial cells, human vascular smooth muscle cells, kidney tubular cells and K-562 cell line.

Subunit Structure:

Heterodimer of an alpha and a beta subunit. Found in a complex with ADD2, DMTN and SLC2A1. Interacts with SLC2A1.

Family&Domains:

Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.

Belongs to the aldolase class II family. Adducin subfamily.

Restrictive clause

 

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