IRS1 Antibody - #AF6273

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Product: IRS1 Antibody
Catalog: AF6273
Description: Rabbit polyclonal antibody to IRS1
Application: WB IHC IF/ICC
Cited expt.: WB, IHC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 180kDa; 132kD(Calculated).
Uniprot: P35568
RRID: AB_2835128

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(91%), Zebrafish(88%), Bovine(91%), Horse(100%), Sheep(91%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
IRS1 Antibody detects endogenous levels of total IRS1.
RRID:
AB_2835128
Cite Format: Affinity Biosciences Cat# AF6273, RRID:AB_2835128.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

HIRS 1; HIRS1; Insulin receptor substrate 1; IRS 1; IRS-1; IRS1; IRS1_HUMAN; OTTHUMP00000164234;

Immunogens

Immunogen:

A synthesized peptide derived from human IRS1, corresponding to a region within the internal amino acids.

Uniprot:

P35568(IRS1_HUMAN) >>Visit HPA database.

Gene(ID):
IRS1(3667)
Description:
IRS-1 is an adaptor protein that is one of the major substrates of the insulin receptor kinase. Contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain-containing proteins including phosphatidylinositol 3-kinase p85 subunit and GRB-2.
Sequence:
MASPPESDGFSDVRKVGYLRKPKSMHKRFFVLRAASEAGGPARLEYYENEKKWRHKSSAPKRSIPLESCFNINKRADSKNKHLVALYTRDEHFAIAADSEAEQDSWYQALLQLHNRAKGHHDGAAALGAGGGGGSCSGSSGLGEAGEDLSYGDVPPGPAFKEVWQVILKPKGLGQTKNLIGIYRLCLTSKTISFVKLNSEAAAVVLQLMNIRRCGHSENFFFIEVGRSAVTGPGEFWMQVDDSVVAQNMHETILEAMRAMSDEFRPRSKSQSSSNCSNPISVPLRRHHLNNPPPSQVGLTRRSRTESITATSPASMVGGKPGSFRVRASSDGEGTMSRPASVDGSPVSPSTNRTHAHRHRGSARLHPPLNHSRSIPMPASRCSPSATSPVSLSSSSTSGHGSTSDCLFPRRSSASVSGSPSDGGFISSDEYGSSPCDFRSSFRSVTPDSLGHTPPARGEEELSNYICMGGKGPSTLTAPNGHYILSRGGNGHRCTPGTGLGTSPALAGDEAASAADLDNRFRKRTHSAGTSPTITHQKTPSQSSVASIEEYTEMMPAYPPGGGSGGRLPGHRHSAFVPTRSYPEEGLEMHPLERRGGHHRPDSSTLHTDDGYMPMSPGVAPVPSGRKGSGDYMPMSPKSVSAPQQIINPIRRHPQRVDPNGYMMMSPSGGCSPDIGGGPSSSSSSSNAVPSGTSYGKLWTNGVGGHHSHVLPHPKPPVESSGGKLLPCTGDYMNMSPVGDSNTSSPSDCYYGPEDPQHKPVLSYYSLPRSFKHTQRPGEPEEGARHQHLRLSTSSGRLLYAATADDSSSSTSSDSLGGGYCGARLEPSLPHPHHQVLQPHLPRKVDTAAQTNSRLARPTRLSLGDPKASTLPRAREQQQQQQPLLHPPEPKSPGEYVNIEFGSDQSGYLSGPVAFHSSPSVRCPSQLQPAPREEETGTEEYMKMDLGPGRRAAWQESTGVEMGRLGPAPPGAASICRPTRAVPSSRGDYMTMQMSCPRQSYVDTSPAAPVSYADMRTGIAAEEVSLPRATMAAASSSSAASASPTGPQGAAELAAHSSLLGGPQGPGGMSAFTRVNLSPNRNQSAKVIRADPQGCRRRHSSETFSSTPSATRVGNTVPFGAGAAVGGGGGSSSSSEDVKRHSSASFENVWLRPGELGGAPKEPAKLCGAAGGLENGLNYIDLDLVKDFKQCPQECTPEPQPPPPPPPHQPLGSGESSSTRRSSEDLSAYASISFQKQPEDRQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Pig
91
Bovine
91
Sheep
91
Zebrafish
88
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).

PTMs:

Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor (By similarity). Phosphorylation of Tyr-896 is required for GRB2-binding (By similarity). Phosphorylated by ALK. Phosphorylated at Ser-270, Ser-307, Ser-636 and Ser-1101 by RPS6KB1; phosphorylation induces accelerated degradation of IRS1. Phosphorylated on tyrosine residues in response to insulin. In skeletal muscles, dephosphorylated on Tyr-612 by TNS2 under anabolic conditions; dephosphorylation results in the proteasomal degradation of IRS1.

Ubiquitinated by the Cul7-RING(FBXW8) complex in a mTOR-dependent manner, leading to its degradation: the Cul7-RING(FBXW8) complex recognizes and binds IRS1 previously phosphorylated by S6 kinase (RPS6KB1 or RPS6KB2).

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway - multiple species.   (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.

References

1). Arsenic induces hepatic insulin resistance via mtROS-NLRP3 inflammasome pathway. JOURNAL OF HAZARDOUS MATERIALS, 2020 (PubMed: 32544768) [IF=12.2]

Application: WB    Species: rat    Sample: liver

Fig.2 The effect of NaAsO2 on mitophagy, ox-mtDNA and NLRP3 inflammation in rats liver. Male SD rats were treated with 2.5, 5 mg/kg of NaAsO2 for 3 months. Liver coefficient (A). The level of serum ALT and AST were determined by commercial reagent kits (B-C). H&E staining of liver sections after NaAsO2 administration (D). scale bar = 500 μm. NAS in rat liver (E). Cytosolic fractions were analyzed by Western blot analysis. GAPDH was used as an internal control. The relative expression of MPO was shown as the percentage of GAPDH (F-G). The level of ox￾mtDNA was measured with an ELISA kit (H). Mitochondria fractions were analyzed by Western blot analysis. VDAC1, mitochondria marker protein, was used as an Journal Pre-proof internal control. The protein level and densitometric analyses of PINK1, Parkin, LC3B in rats liver tissues (I-L). The protein level and densitometric analyses of NLRP3, IL-1β, IL-18 expressed in rat liver tissues (M-T). Results are mean ± SEM of 5 rats. *P < 0.05 compare with the control group.
2). Insulin promotes the bone formation capability of human dental pulp stem cells through attenuating the IIS/PI3K/AKT/mTOR pathway axis. Stem cell research & therapy, 2024 (PubMed: 39075596) [IF=7.5]

Application: WB    Species: Human    Sample: DPSCs

Fig. 4 10− 6 M insulin inhibits the gene and protein expressions of the IIS-related receptors and substrates in human DPSCs. A 10− 6 M insulin down-regulated the mRNA levels of INSR, IGF1R, and IRS1 in DPSCs at day 3 and day 7. B 10− 6 M insulin inhibited the protein expressions of INSR, IGF1R, and IRS1 in DPSCs at day 7. Representative western blotting (left) and quantification analysis (right). Full-length blots/gels are presented in Supplementary Fig. 2. Data are expressed as the mean ± SD of n = 3.
3). Probiotic Yogurt Alleviates High-Fat Diet-Induced Lipid Accumulation and Insulin Resistance in Mice via the Adiponectin Pathway. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2023 (PubMed: 36695046) [IF=5.7]
4). Anti-diabetes effect of chronic intermittent hypobaric hypoxia through improving liver insulin resistance in diabetic rats. LIFE SCIENCES, 2016 (PubMed: 26883978) [IF=5.2]

Application: WB    Species:    Sample:

5). Silencing of ANGPTL8 Alleviates Insulin Resistance in Trophoblast Cells. Frontiers in Endocrinology, 2021 (PubMed: 34163433) [IF=3.9]

Application: WB    Species: mouse    Sample: placenta

FIGURE 1 | Angiopoietin like-8 (ANGPTL8) was increased in serum and placenta tissues of gestational diabetes mellitus (GDM) mice.(J) Western blot was used to determine the levels of insulin signaling related molecules, p-IRb(Tyr1361), IRb, p-IRS-1(Ser307), p-IRS-1(Tyr896), IRS-1, p-Akt and Akt in placenta tissues.

Application: WB    Species: Mice    Sample: serum and placenta tissue

Figure 1 Angiopoietin like-8 (ANGPTL8) was increased in serum and placenta tissues of gestational diabetes mellitus (GDM) mice. (A) The mice were treated as described in the chart. (B) The body weight of mice in normal fat diet (NFD) and high fat diet (HFD) groups. (C) Oral glucose tolerance test (OGTT) was performed at gestational day (GD)0.5, 11.5 and 16.5. (D, E) Fasting blood glucose and insulin levels were measured at GD18.5. (F) Homeostasis model assessment insulin resistance (HOMA-IR) was calculated as follow: HOMA-IR= blood glucose (mM)×blood insulin (mU/l)/22.5. (G) The contents of triglyceride (TG), total cholesterol (TC), high density lipoprotein (HDL-C) and low density lipoprotein (LDL-C) in serum were detected. (H) HE staining was performed to detect the pathological changes in labyrinth zone of placenta tissues. (I) Periodic acid Schiff (PAS) staining was carried out to detect the glycogen accumulation in labyrinth zone of placenta tissues. (J) Western blot was used to determine the levels of insulin signaling related molecules, p-IRβ(Tyr1361), IRβ, p-IRS-1(Ser307), p-IRS-1(Tyr896), IRS-1, p-Akt and Akt in placenta tissues. (K) The expression levels of glucose transporter 1 (GLUT1) and GLUT4 in placenta tissues. (L) The serum level of ANGPTL8 in mice. (M, N) The mRNA and protein levels of ANGPTL8 in placenta tissues. (the scale bar represents 100 μm; **p < 0.01, ***p < 0.001 vs. NFD).
6). Periplocymarin Induced Colorectal Cancer Cells Apoptosis Via Impairing PI3K/AKT Pathway. Frontiers in Oncology, 2021 (PubMed: 34900704) [IF=3.5]

Application: WB    Species: Human    Sample: HCT 116 RKO cells

Figure 6 Periplocymarin (PPM) modulates gene expression involved in PI3K/AKT signaling pathway. PPM treatment altered the expression levels of IRS1, PI3K, AKT, p-PI3K, and p-AKT in HCT 116 (A) and RKO cells (B). Values are shown as means ± SEM. *P < 0.05, **P < 0.01, vs. untreated control group.

Application: IHC    Species: Mice    Sample: tumors

Figure 7 The effect of periplocymarin (PPM) on colorectal cancer cells in nude mice. (A) Images of tumors derived from mice in each group. (B) Tumor volumes were calculated in each group every 3 days from days 1 to 21. (C) Tumor weights were measured on day 21. (D) Hematoxylin/eosin-stained sections of tumor tissues derived from mice in each treatment group were presented. Magnification: 200×. Scale bar, 150 μm. The expression of Bax, cleaved caspase-3, IRS1, p-PI3K, and p-AKT in tumor tissues of mice from each treatment group was examined by immunohistochemistry. Magnification: 400×. Scale bar, 70 μm. *P < 0.05, **P < 0.01, vs. untreated control group. # P < 0.05, vs. 5-FU treatment group. & P < 0.05, vs. PPM treatment group. n =6 per group.
7). Metabolomics Study of Whole-body Vibration on Lipid Metabolism of Skeletal Muscle in Aging Mice. INTERNATIONAL JOURNAL OF SPORTS MEDICINE, 2020 (PubMed: 33124015) [IF=2.0]
8). The Aqueous Extract of Gynura divaricata (L.) DC. Improves Glucose and Lipid Metabolism and Ameliorates Type 2 Diabetes Mellitus. Evidence-based Complementary and Alternative Medicine, 2018 (PubMed: 29599810)
9). Exploring the anti-diabetic effects and the underlying mechanisms of ethyl acetate extract from Sophora flavescens by integrating network pharmacology and pharmacological evaluation. Traditional Medicine Research, 2022

Application: WB    Species: Rat    Sample:

Figure 7 Western blot analysis of the proteins. (A) Representative bands of IRS-1, p-PI3K, PI3K, p-AKT, AKT and GLUT4 were shown.

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