NCE2 Antibody - #DF14306

(3)  
Product: NCE2 Antibody
Catalog: DF14306
Description: Rabbit polyclonal antibody to NCE2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 21kD(Calculated).
Uniprot: Q969M7

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
NCE2 Antibody detects endogenous levels of total NCE2.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

NCE2; NEDD8 carrier protein UBE2F; NEDD8 conjugating enzyme; NEDD8 conjugating enzyme UBE2F; NEDD8 protein ligase UBE2F; NEDD8-conjugating enzyme 2; NEDD8-conjugating enzyme UBE2F; ube2f; UBE2F_HUMAN; Ubiquitin conjugating enzyme E2F; Ubiquitin conjugating enzyme E2F (putative); Ubiquitin-conjugating enzyme E2 F;

Immunogens

Immunogen:

A synthesized peptide derived from Human NCE2.

Uniprot:

Q969M7(UBE2F_HUMAN) >>Visit HPA database.

Gene(ID):
UBE2F(140739)
Expression:
Q969M7 UBE2F_HUMAN:

Widely expressed (at protein level).

Sequence:
MLTLASKLKRDDGLKGSRTAATASDSTRRVSVRDKLLVKEVAELEANLPCTCKVHFPDPNKLHCFQLTVTPDEGYYQGGKFQFETEVPDAYNMVPPKVKCLTKIWHPNITETGEICLSLLREHSIDGTGWAPTRTLKDVVWGLNSLFTDLLNFDDPLNIEAAEHHLRDKEDFRNKVDDYIKRYAR

Research Backgrounds

Function:

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.

PTMs:

The acetylation of Met-1 increases affinity for DCUN1D3 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins.

Tissue Specificity:

Widely expressed (at protein level).

Subunit Structure:

Interacts with UBA3 and RBX2.

Family&Domains:

Belongs to the ubiquitin-conjugating enzyme family. UBE2F subfamily.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

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