DNase I Antibody - #DF14243

(2)  
Product: DNase I Antibody
Catalog: DF14243
Description: Rabbit polyclonal antibody to DNase I
Application: ELISA(peptide)
Reactivity: Human, Mouse, Rat
Mol.Wt.: 30~40kD; 31kD(Calculated).
Uniprot: P24855

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
DNase I Antibody detects endogenous levels of total DNase I.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Deoxyribonuclease 1; Deoxyribonuclease I; Deoxyribonuclease-1; Deoxyribonuclease1; DeoxyribonucleaseI; DNAS1_HUMAN; DNASE 1; DNase I; DNase I lysosomal; DNASE1; DNaseI; DNL 1; DNL1; Dornase alfa; DRNI; FLJ38093; Human urine deoxyribonuclease I;

Immunogens

Immunogen:

A synthesized peptide derived from Human DNase I.

Uniprot:

P24855(DNAS1_HUMAN) >>Visit HPA database.

Gene(ID):
DNASE1(1773)
Expression:
P24855 DNAS1_HUMAN:

Principally in tissues of the digestive system. Highest levels found in urine, but also relatively abundant in semen and saliva.

Sequence:
MRGMKLLGALLALAALLQGAVSLKIAAFNIQTFGETKMSNATLVSYIVQILSRYDIALVQEVRDSHLTAVGKLLDNLNQDAPDTYHYVVSEPLGRNSYKERYLFVYRPDQVSAVDSYYYDDGCEPCGNDTFNREPAIVRFFSRFTEVREFAIVPLHAAPGDAVAEIDALYDVYLDVQEKWGLEDVMLMGDFNAGCSYVRPSQWSSIRLWTSPTFQWLIPDSADTTATPTHCAYDRIVVAGMLLRGAVVPDSALPFNFQAAYGLSDQLAQAISDHYPVEVMLK

Research Backgrounds

Function:

Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs. Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA (By similarity). Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization (By similarity). Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs) (By similarity). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (By similarity).

Subcellular Location:

Secreted. Nucleus envelope.
Note: Secretory protein, stored in zymogen granules and found in the nuclear envelope.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Principally in tissues of the digestive system. Highest levels found in urine, but also relatively abundant in semen and saliva.

Family&Domains:

Belongs to the DNase I family.

References

1). Multistage Cooperative Nanodrug Combined with PD‐L1 for Enhancing Antitumor Chemoimmunotherapy. Advanced Healthcare Materials, 2021 (PubMed: 34382363) [IF=10.0]
2). Self-Assembled Immunostimulatory Nanodrug Combined with PD-L1 for Boosting Anti-Tumor Chemoimmunotherapy. , 2022

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