SLC39A14 Antibody - #DF14224

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Product: SLC39A14 Antibody
Catalog: DF14224
Description: Rabbit polyclonal antibody to SLC39A14
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 54kD; 54kD(Calculated).
Uniprot: Q15043

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
SLC39A14 Antibody detects endogenous levels of total SLC39A14.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cig19; KIAA0062; LIV-1 subfamily of ZIP zinc transporter 4; LZT-Hs4; NET34; S39AE_HUMAN; SLC39A14; Solute carrier family 39 (zinc transporter), member 14; Solute carrier family 39 member 14; Zinc transporter ZIP14; ZIP-14; ZIP14; Zrt Irt like protein 14; Zrt- and Irt-like protein 14;

Immunogens

Immunogen:

A synthesized peptide derived from Human SLC39A14.

Uniprot:

Q15043(S39AE_HUMAN) >>Visit HPA database.

Gene(ID):
SLC39A14(23516)
Expression:
Q15043 S39AE_HUMAN:

Expressed in liver and in brain by large neurons in the globus pallidus, the insular cortex and the dentate nucleus and to a lower extent in the putamen and the caudate nucleus (at protein level) (PubMed:27231142). Isoform 1 is ubiquitously expressed, with increased expression in liver, pancreas, fetal liver, thyroid gland, left and right ventricle, right atrium and fetal heart. Weakly expressed in spleen, thymus, and peripheral blood leukocytes (PubMed:15642354, PubMed:7584044, PubMed:27231142). Isoform 3 is widely expressed but not detected in brain, heart, skeletal muscle and fetal skin (PubMed:27231142). Expressed in osteoblasts and giant osteoclast-like cells, but not in osteocytes found osteoblastoma and giant cell tumors (at protein level) (PubMed:29621230).

Sequence:
MKLLLLHPAFQSCLLLTLLGLWRTTPEAHASSLGAPAISAASFLQDLIHRYGEGDSLTLQQLKALLNHLDVGVGRGNVTQHVQGHRNLSTCFSSGDLFTAHNFSEQSRIGSSELQEFCPTILQQLDSRACTSENQENEENEQTEEGRPSAVEVWGYGLLCVTVISLCSLLGASVVPFMKKTFYKRLLLYFIALAIGTLYSNALFQLIPEAFGFNPLEDYYVSKSAVVFGGFYLFFFTEKILKILLKQKNEHHHGHSHYASESLPSKKDQEEGVMEKLQNGDLDHMIPQHCSSELDGKAPMVDEKVIVGSLSVQDLQASQSACYWLKGVRYSDIGTLAWMITLSDGLHNFIDGLAIGASFTVSVFQGISTSVAILCEEFPHELGDFVILLNAGMSIQQALFFNFLSACCCYLGLAFGILAGSHFSANWIFALAGGMFLYISLADMFPEMNEVCQEDERKGSILIPFIIQNLGLLTGFTIMVVLTMYSGQIQIG

Research Backgrounds

Function:

Broad-scope metal ion transporter with a preference for zinc uptake. Also mediates cellular uptake of nontransferrin-bound iron.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Cytoplasm. Cell projection>Lamellipodium.
Note: Localized to the plasma membrane and also found colocalized with F-actin concentrated on lamellipodiae.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in liver and in brain by large neurons in the globus pallidus, the insular cortex and the dentate nucleus and to a lower extent in the putamen and the caudate nucleus (at protein level). Isoform 1 is ubiquitously expressed, with increased expression in liver, pancreas, fetal liver, thyroid gland, left and right ventricle, right atrium and fetal heart. Weakly expressed in spleen, thymus, and peripheral blood leukocytes. Isoform 3 is widely expressed but not detected in brain, heart, skeletal muscle and fetal skin. Expressed in osteoblasts and giant osteoclast-like cells, but not in osteocytes found osteoblastoma and giant cell tumors (at protein level).

Subunit Structure:

Homotrimer.

Family&Domains:

Belongs to the ZIP transporter (TC 2.A.5) family.

Research Fields

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

References

1). Identification and analysis of a prognostic ferroptosis and iron-metabolism signature for esophageal squamous cell carcinoma. Journal of Cancer, 2023 (PubMed: 35371305) [IF=3.9]

Application: IF/ICC    Species: Human    Sample: ESCC

Figure 4 Immunofluorescence of PRNP (green), SLC39A8 (red), LCN2 (red), ATP6V0A1 (green), SLC3A2 (red), and SLC39A14 (green) in ESCC samples and normal tissues.
2). SLC39A14 may be a characteristic gene affecting the development of Barrett's esophagus. Research Square, 2023

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