RDH5 Antibody - #DF14183

(2)  
Product: RDH5 Antibody
Catalog: DF14183
Description: Rabbit polyclonal antibody to RDH5
Application: IHC IF/ICC
Reactivity: Human
Mol.Wt.: 35kD(Calculated).
Uniprot: Q92781

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
RDH5 Antibody detects endogenous levels of total RDH5.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

11 cis RDH; 11 cis retinol dehydrogenase; 9 cis retinol specific dehydrogenase; FLJ39337; FLJ97089; HSD17B9; RDH 5; RDH1; Retinol dehydrogenase 1; Retinol dehydrogenase 5 (11 cis/9 cis); Retinol dehydrogenase 5; SDR9C5; Short chain dehydrogenase/reductase family 9C member 5;

Immunogens

Immunogen:

A synthesized peptide derived from Human RDH5.

Uniprot:

Q92781(RDH5_HUMAN) >>Visit HPA database.

Gene(ID):
RDH5(5959)
Expression:
Q92781 RDH5_HUMAN:

Widely expressed. In the eye, abundant in the retinal pigment epithelium.

Sequence:
MWLPLLLGALLWAVLWLLRDRQSLPASNAFVFITGCDSGFGRLLALQLDQRGFRVLASCLTPSGAEDLQRVASSRLHTTLLDITDPQSVQQAAKWVEMHVKEAGLFGLVNNAGVAGIIGPTPWLTRDDFQRVLNVNTMGPIGVTLALLPLLQQARGRVINITSVLGRLAANGGGYCVSKFGLEAFSDSLRRDVAHFGIRVSIVEPGFFRTPVTNLESLEKTLQACWARLPPATQAHYGGAFLTKYLKMQQRIMNLICDPDLTKVSRCLEHALTARHPRTRYSPGWDAKLLWLPASYLPASLVDAVLTWVLPKPAQAVY

Research Backgrounds

Function:

Catalyzes the oxidation of cis-isomers of retinol, including 11-cis-, 9-cis-, and 13-cis-retinol in an NAD-dependent manner. Has no activity towards all-trans retinal (By similarity). Plays a significant role in 11-cis retinol oxidation in the retinal pigment epithelium cells (RPE). Also recognizes steroids (androsterone, androstanediol) as its substrates.

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein>Lumenal side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. In the eye, abundant in the retinal pigment epithelium.

Subunit Structure:

Homodimer.

Family&Domains:

The last 8 amino acids of the C-terminal tail are important for a proper localization as well as for the in vivo enzymatic activity.

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Research Fields

· Metabolism > Metabolism of cofactors and vitamins > Retinol metabolism.

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