SENP1 Antibody - #AF0275

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Product: SENP1 Antibody
Catalog: AF0275
Description: Rabbit polyclonal antibody to SENP1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 73kDa; 73kD(Calculated).
Uniprot: Q9P0U3
RRID: AB_2833445

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(91%), Dog(100%)
Clonality:
Polyclonal
Specificity:
SENP1 Antibody detects endogenous levels of total SENP1.
RRID:
AB_2833445
Cite Format: Affinity Biosciences Cat# AF0275, RRID:AB_2833445.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

SENP 1; SENP1; SENP1_HUMAN; Sentrin specific protease 1; Sentrin-specific protease 1; Sentrin/SUMO specific protease 1; Sentrin/SUMO specific protease; Sentrin/SUMO specific protease SENP 1; Sentrin/SUMO specific protease SENP1; Sentrin/SUMO-specific protease SENP1; SUMO1/sentrin specific peptidase 1; SUMO1/sentrin specific protease 1; SuPr 2; SuPr2;

Immunogens

Immunogen:
Uniprot:

Q9P0U3(SENP1_HUMAN) >>Visit HPA database.

Gene(ID):
SENP1(29843)
Expression:
Q9P0U3 SENP1_HUMAN:

Highly expressed in testis. Expressed at lower levels in thymus, pancreas, spleen, liver, ovary and small intestine.

Description:
SENP1 Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. Deconjugates SUMO1 from HIPK2. Deconjugates SUMO1 from HDAC1, which decreases its transcriptional repression activity.
Sequence:
MDDIADRMRMDAGEVTLVNHNSVFKTHLLPQTGFPEDQLSLSDQQILSSRQGHLDRSFTCSTRSAAYNPSYYSDNPSSDSFLGSGDLRTFGQSANGQWRNSTPSSSSSLQKSRNSRSLYLETRKTSSGLSNSFAGKSNHHCHVSAYEKSFPIKPVPSPSWSGSCRRSLLSPKKTQRRHVSTAEETVQEEEREIYRQLLQMVTGKQFTIAKPTTHFPLHLSRCLSSSKNTLKDSLFKNGNSCASQIIGSDTSSSGSASILTNQEQLSHSVYSLSSYTPDVAFGSKDSGTLHHPHHHHSVPHQPDNLAASNTQSEGSDSVILLKVKDSQTPTPSSTFFQAELWIKELTSVYDSRARERLRQIEEQKALALQLQNQRLQEREHSVHDSVELHLRVPLEKEIPVTVVQETQKKGHKLTDSEDEFPEITEEMEKEIKNVFRNGNQDEVLSEAFRLTITRKDIQTLNHLNWLNDEIINFYMNMLMERSKEKGLPSVHAFNTFFFTKLKTAGYQAVKRWTKKVDVFSVDILLVPIHLGVHWCLAVVDFRKKNITYYDSMGGINNEACRILLQYLKQESIDKKRKEFDTNGWQLFSKKSQEIPQQMNGSDCGMFACKYADCITKDRPINFTQQHMPYFRKRMVWEILHRKLL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Horse
92
Rabbit
91
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9P0U3 As Substrate

Site PTM Type Enzyme
S57 Phosphorylation
T59 Phosphorylation
S78 Phosphorylation
S80 Phosphorylation
T102 Phosphorylation
K111 Ubiquitination
S117 Phosphorylation
K124 Methylation
K124 Ubiquitination
S126 Phosphorylation
S127 Phosphorylation
S130 Phosphorylation
S132 Phosphorylation
K136 Methylation
K136 Ubiquitination
S149 Phosphorylation
K153 Ubiquitination
S157 Phosphorylation
S159 Phosphorylation
S161 Phosphorylation
S167 Phosphorylation
S170 Phosphorylation
S180 Phosphorylation
K210 Ubiquitination
S224 Phosphorylation
S226 Phosphorylation
K227 Ubiquitination
K231 Ubiquitination
S233 Phosphorylation
T328 Phosphorylation
T330 Phosphorylation
Y349 Phosphorylation
K364 Acetylation
K364 Ubiquitination
S381 Phosphorylation
K396 Acetylation
K396 Ubiquitination
T414 Phosphorylation
S416 Phosphorylation
K429 Acetylation
K429 Ubiquitination
K432 Ubiquitination
K502 Ubiquitination
K510 Ubiquitination
K577 Ubiquitination
K609 Ubiquitination
K616 Ubiquitination

Research Backgrounds

Function:

Protease that catalyzes two essential functions in the SUMO pathway. The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides, SUMO1, SUMO2 and SUMO3 leading to the mature form of the proteins. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins, by cleaving an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein. Deconjugates SUMO1 from HIPK2. Deconjugates SUMO1 from HDAC1 and BHLHE40/DEC1, which decreases its transcriptional repression activity. Deconjugates SUMO1 from CLOCK, which decreases its transcriptional activation activity. Deconjugates SUMO2 from MTA1. Deconjugates SUMO1 from METTL3. Desumoylates CCAR2 which decreases its interaction with SIRT1. Deconjugates SUMO1 from GPS2.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Shuttles between cytoplasm and nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in testis. Expressed at lower levels in thymus, pancreas, spleen, liver, ovary and small intestine.

Subunit Structure:

Interacts with MTA1. Interacts with CCAR2 (via N-terminus).

Family&Domains:

Belongs to the peptidase C48 family.

References

1). Hypoxia maintains the fenestration of liver sinusoidal endothelial cells and promotes their proliferation through the SENP1/HIF-1α/VEGF signaling axis. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (PubMed: 33445109) [IF=3.1]

Application: WB    Species: mouse    Sample: Liver sinusoidal endothelial cells (LSECs)

Fig. 2. After hypoxic culture of mouse LSECs, the expressions of SENP1,HIF-1a,and VEGF are all up-regulated.(A)(B)(C)real-time PCR showing SENP1 or HIF-1a or VEGF mRNA after a certain period of hypoxia culture.The date are presented as mean ± SEM.**p < 0.01,***p < 0.001,****p < 0.0001,n ¼ 3per group.(D)Western results showing the expression level of SENP1 and HIF-1a after a certain time of hypoxia culture.(E)(F) ratio of SENP1 or HIF-1a vs b-actin in western results.The date are presented as mean ± SEM.*p < 0.05,**p < 0.01,***p < 0.001,n ¼ 3 per group.(G) ELISA assay of VEGF production in LSECs after a certain time of hypoxia culture.The date are presented as mean ± SEM.****p < 0.0001,n ¼ 3 per group.

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