NHP2L1 Antibody - #DF14141

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Product: NHP2L1 Antibody
Catalog: DF14141
Description: Rabbit polyclonal antibody to NHP2L1
Application: IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 14kD; 14kD(Calculated).
Uniprot: P55769

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 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
NHP2L1 Antibody detects endogenous levels of total NHP2L1.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

[U4/U6.U5] tri snRNP; 15.5 kD RNA binding protein; High mobility group like nuclear protein 2 homolog 1; hSNU13; NHP2 non histone chromosome protein 2 like 1 (S. cerevisiae); NHP2L 1; NHP2L1; NHPX; OTK27; SNU13;

Immunogens

Immunogen:

A synthesized peptide derived from Human NHP2L1.

Uniprot:

P55769(NH2L1_HUMAN) >>Visit HPA database.

Gene(ID):
SNU13(4809)
Expression:
P55769 NH2L1_HUMAN:

Ubiquitous.

Sequence:
MTEADVNPKAYPLADAHLTKKLLDLVQQSCNYKQLRKGANEATKTLNRGISEFIVMAADAEPLEIILHLPLLCEDKNVPYVFVRSKQALGRACGVSRPVIACSVTIKEGSQLKQQIQSIQQSIERLLV

Research Backgrounds

Function:

Involved in pre-mRNA splicing as component of the spliceosome. Binds to the 5'-stem-loop of U4 snRNA and thereby contributes to spliceosome assembly. The protein undergoes a conformational change upon RNA-binding.

Subcellular Location:

Nucleus. Nucleus>Nucleolus.
Note: Concentrated in the dense fibrillar component of the nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Identified in the spliceosome B complex. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, WDR57, SNRNP40, DDX23, CD2BP2, PPIH, NHP2L1, EFTUD2, SART1 and USP39. Interacts with RAD17 and PRPF31. The complex formed by SNU13 and PRPF31 binds U4 snRNA. The complex formed by SNU13 and PRPF31 binds also U4atac snRNA, a characteristic component of specific, less abundant spliceosomal complexes.

Family&Domains:

Belongs to the eukaryotic ribosomal protein eL8 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

· Genetic Information Processing > Transcription > Spliceosome.

Restrictive clause

 

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