TRAK1 Antibody | Affinity Biosciences

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DF14119 at 1/100 staining rat testis tissue by IHC-P.

Product:	TRAK1 Antibody
Catalog:	DF14119
Description:	Rabbit polyclonal antibody to TRAK1
Application:	IHC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	70kD; 106kD(Calculated).
Uniprot:	Q9UPV9

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

TRAK1 Antibody detects endogenous levels of total TRAK1.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

106 kDa O GlcNAc transferase interacting protein; KIAA1042; OGT(O Glc NAc transferase) interacting protein 106 KDa; OIP106; Trafficking kinesin binding protein 1; Trafficking protein kinesin binding 1; TRAK 1;

Immunogens

Immunogen:

A synthesized peptide derived from Human TRAK1.

Uniprot:

Q9UPV9(TRAK1_HUMAN) >>Visit HPA database.

Gene(ID):

TRAK1(22906)

Expression:

Q9UPV9 TRAK1_HUMAN:

High expression in spinal cord and moderate expression in all other tissues and specific brain regions examined. Expressed in all cell lines examined.

Sequence:

Q9UPV9 TRAK1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MALVFQFGQPVRAQPLPGLCHGKLIRTNACDVCNSTDLPEVEIISLLEEQLPHYKLRADTIYGYDHDDWLHTPLISPDANIDLTTEQIEETLKYFLLCAERVGQMTKTYNDIDAVTRLLEEKERDLELAARIGQSLLKKNKTLTERNELLEEQVEHIREEVSQLRHELSMKDELLQFYTSAAEESEPESVCSTPLKRNESSSSVQNYFHLDSLQKKLKDLEEENVVLRSEASQLKTETITYEEKEQQLVNDCVKELRDANVQIASISEELAKKTEDAARQQEEITHLLSQIVDLQKKAKACAVENEELVQHLGAAKDAQRQLTAELRELEDKYAECMEMLHEAQEELKNLRNKTMPNTTSRRYHSLGLFPMDSLAAEIEGTMRKELQLEEAESPDITHQKRVFETVRNINQVVKQRSLTPSPMNIPGSNQSSAMNSLLSSCVSTPRSSFYGSDIGNVVLDNKTNSIILETEAADLGNDERSKKPGTPGTPGSHDLETALRRLSLRRENYLSERRFFEEEQERKLQELAEKGELRSGSLTPTESIMSLGTHSRFSEFTGFSGMSFSSRSYLPEKLQIVKPLEGSATLHHWQQLAQPHLGGILDPRPGVVTKGFRTLDVDLDEVYCLNDFEEDDTGDHISLPRLATSTPVQHPETSAHHPGKCMSQTNSTFTFTTCRILHPSDELTRVTPSLNSAPTPACGSTSHLKSTPVATPCTPRRLSLAESFTNTRESTTTMSTSLGLVWLLKERGISAAVYDPQSWDRAGRGSLLHSYTPKMAVIPSTPPNSPMQTPTSSPPSFEFKCTSPPYDNFLASKPASSILREVREKNVRSSESQTDVSVSNLNLVDKVRRFGVAKVVNSGRAHVPTLTEEQGPLLCGPPGPAPALVPRGLVPEGLPLRCPTVTSAIGGLQLNSGIRRNRSFPTMVGSSMQMKAPVTLTSGILMGAKLSKQTSLR

Research Backgrounds

Q9UPV9_TRAK1_HUMAN

Function:

Involved in the regulation of endosome-to-lysosome trafficking, including endocytic trafficking of EGF-EGFR complexes and GABA-A receptors. Involved in mitochondrial motility. When O-glycosylated, abolishes mitochondrial motility. Crucial for recruiting OGT to the mitochondrial surface of neuronal processes. TRAK1 and RHOT form an essential protein complex that links KIF5 to mitochondria for light chain-independent, anterograde transport of mitochondria (By similarity).

PTMs:

O-glycosylated. Glycosylated by OGT; glycosylation in response to increased extracellular glucose levels is required for and leads to regulation of mitochondrial motility by OGT.

Subcellular Location:

Cytoplasm. Nucleus. Mitochondrion. Early endosome. Endosome. Mitochondrion membrane. Cytoplasm>Cell cortex.
Note: Predominantly associated with early endosome. The localization to early endosomes depends on its interaction with HGS/HRS (PubMed:18675823). Colocalizes with MGARP at the mitochondria (PubMed:19528298).

Tissue Specificity:

High expression in spinal cord and moderate expression in all other tissues and specific brain regions examined. Expressed in all cell lines examined.

Subunit Structure:

Interacts with RHOT1 and RHOT2. Found in a complex with KIF5B, OGT, RHOT1 and RHOT2. Interacts with HGS. Interacts with GABRA1 (By similarity). Interacts with KIF5C. Interacts with OGT; stable interaction is not required for glycosylation of this protein by OGT. Isoform 1 interacts with OGT.

Family&Domains:

The C-terminal region is required for the early endosomal and mitochondrial localization.

Belongs to the milton family.

Restrictive clause

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TRAK1 Antibody - #DF14119