MKP1/2 Antibody - #AF6243
(2)
(2)
| Product: | MKP1/2 Antibody |
| Catalog: | AF6243 |
| Description: | Rabbit polyclonal antibody to MKP1/2 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 41kDa; 39kD,43kD(Calculated). |
| Uniprot: | P28562 | Q13115 |
| RRID: | AB_2835107 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF6243, RRID:AB_2835107.
Fold/Unfold
CL 100; CL100; Dual Specificity Phosphatase 1; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; DUS1_HUMAN; DUSP 1; Dusp1; HVH1; MAP kinase phosphatase 1; Mitogen-activated protein kinase phosphatase 1; MKP-1; MKP1; Protein tyrosine phosphatase CL100; Protein-tyrosine phosphatase CL100; PTPN10; Serine/threonine specific protein phosphatase; VH1; Dual specificity phosphatase 4; Dual specificity protein phosphatase 4; Dual specificity protein phosphatase hVH2; DUS4_HUMAN; DUSP 4; Dusp4; HVH 2; HVH2; MAP kinase phosphatase 2; Mitogen activated protein kinase phosphatase 2; Mitogen-activated protein kinase phosphatase 2; MKP 2; MKP-2; MKP2; Serine/threonine specific protein phosphatase; TYP; Typ1; VH 2; VH1 homologous phosphatase 2; VH2;
Immunogens
Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.
- P28562 DUS1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVMEVGTLDAGGLRALLGERAAQCLLLDCRSFFAFNAGHIAGSVNVRFSTIVRRRAKGAMGLEHIVPNAELRGRLLAGAYHAVVLLDERSAALDGAKRDGTLALAAGALCREARAAQVFFLKGGYEAFSASCPELCSKQSTPMGLSLPLSTSVPDSAESGCSSCSTPLYDQGGPVEILPFLYLGSAYHASRKDMLDALGITALINVSANCPNHFEGHYQYKSIPVEDNHKADISSWFNEAIDFIDSIKNAGGRVFVHCQAGISRSATICLAYLMRTNRVKLDEAFEFVKQRRSIISPNFSFMGQLLQFESQVLAPHCSAEAGSPAMAVLDRGTSTTTVFNFPVSIPVHSTNSALSYLQSPITTSPSC
- Q13115 DUS4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVTMEELREMDCSVLKRLMNRDENGGGAGGSGSHGTLGLPSGGKCLLLDCRPFLAHSAGYILGSVNVRCNTIVRRRAKGSVSLEQILPAEEEVRARLRSGLYSAVIVYDERSPRAESLREDSTVSLVVQALRRNAERTDICLLKGGYERFSSEYPEFCSKTKALAAIPPPVPPSATEPLDLGCSSCGTPLHDQGGPVEILPFLYLGSAYHAARRDMLDALGITALLNVSSDCPNHFEGHYQYKCIPVEDNHKADISSWFMEAIEYIDAVKDCRGRVLVHCQAGISRSATICLAYLMMKKRVRLEEAFEFVKQRRSIISPNFSFMGQLLQFESQVLATSCAAEAASPSGPLRERGKTPATPTSQFVFSFPVSVGVHSAPSSLPYLHSPITTSPSC
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P28562/Q13115 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K57 | Acetylation | PR:000007102 (EP300) | Uniprot |
| K57 | Ubiquitination | Uniprot | |
| K97 | Acetylation | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| S131 | Phosphorylation | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| T141 | Phosphorylation | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| K221 | Ubiquitination | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| K280 | Ubiquitination | Uniprot | |
| K289 | Ubiquitination | Uniprot | |
| S296 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
| S323 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
| S334 | Phosphorylation | P54646 (PRKAA2) | Uniprot |
| S359 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| S364 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| V2 | Acetylation | Uniprot | |
| K16 | Ubiquitination | Uniprot | |
| Y60 | Phosphorylation | Uniprot | |
| S64 | Phosphorylation | Uniprot | |
| T71 | Phosphorylation | Uniprot | |
| K78 | Ubiquitination | Uniprot | |
| Y102 | Phosphorylation | Uniprot | |
| Y108 | Phosphorylation | Uniprot | |
| S112 | Phosphorylation | Uniprot | |
| Y154 | Phosphorylation | Uniprot | |
| S159 | Phosphorylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| K311 | Ubiquitination | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| T356 | Phosphorylation | Uniprot | |
| T359 | Phosphorylation | Uniprot | |
| S386 | Phosphorylation | Uniprot | |
| S391 | Phosphorylation | Uniprot |
Research Backgrounds
Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.
Phosphorylation at Ser-359 and Ser-364 by MAPK1/ERK2 and MAPK3/ERK1 reduces its rate of degradation.
Nucleus.
Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.
Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
Regulates mitogenic signal transduction by dephosphorylating both Thr and Tyr residues on MAP kinases ERK1 and ERK2.
Phosphorylation in the C-terminus by ERK1/2 inhibits proteasomal degradation and stabilizes the protein.
Nucleus.
Hollow spherical complex composed of 24 subunits with pseudooctahedral symmetry, has a tetramer as the basic unit.
Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
Research Fields
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Organismal Systems > Nervous system > Serotonergic synapse.
References
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